GBB_SCHPO
ID GBB_SCHPO Reviewed; 305 AA.
AC Q10282;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 11-JAN-2001, sequence version 2.
DT 25-MAY-2022, entry version 164.
DE RecName: Full=Guanine nucleotide-binding protein subunit beta;
GN Name=git5; Synonyms=gpb1; ORFNames=pi017, SPBC32H8.07;
OS Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC Schizosaccharomyces.
OX NCBI_TaxID=284812;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=8804400; DOI=10.1007/bf02173201;
RA Kim D.U., Park S.K., Chung K.S., Choi M.U., Yoo H.S.;
RT "The G protein beta subunit Gpb1 of Schizosaccharomyces pombe is a negative
RT regulator of sexual development.";
RL Mol. Gen. Genet. 252:20-32(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=10747045; DOI=10.1093/genetics/154.4.1463;
RA Landry S., Pettit M.T., Apolinario E., Hoffman C.S.;
RT "The fission yeast git5 gene encodes a gbeta subunit required for glucose-
RT triggered adenylate cyclase activation.";
RL Genetics 154:1463-1471(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=10620777;
RX DOI=10.1002/(sici)1097-0061(20000115)16:1<71::aid-yea505>3.0.co;2-5;
RA Machida M., Yamazaki S., Kunihiro S., Tanaka T., Kushida N., Jinno K.,
RA Haikawa Y., Yamazaki J., Yamamoto S., Sekine M., Oguchi A., Nagai Y.,
RA Sakai M., Aoki K., Ogura K., Kudoh Y., Kikuchi H., Zhang M.Q., Yanagida M.;
RT "A 38 kb segment containing the cdc2 gene from the left arm of fission
RT yeast chromosome II: sequence analysis and characterization of the genomic
RT DNA and cDNAs encoded on the segment.";
RL Yeast 16:71-80(2000).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=972 / ATCC 24843;
RX PubMed=11859360; DOI=10.1038/nature724;
RA Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA Nurse P.;
RT "The genome sequence of Schizosaccharomyces pombe.";
RL Nature 415:871-880(2002).
RN [5]
RP FUNCTION.
RX PubMed=16884933; DOI=10.1016/j.fgb.2006.06.005;
RA Goddard A., Ladds G., Forfar R., Davey J.;
RT "Identification of Gnr1p, a negative regulator of G alpha signalling in
RT Schizosaccharomyces pombe, and its complementation by human G beta
RT subunits.";
RL Fungal Genet. Biol. 43:840-851(2006).
RN [6]
RP SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX PubMed=16823372; DOI=10.1038/nbt1222;
RA Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S.,
RA Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S.,
RA Yoshida M.;
RT "ORFeome cloning and global analysis of protein localization in the fission
RT yeast Schizosaccharomyces pombe.";
RL Nat. Biotechnol. 24:841-847(2006).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC as a modulator or transducer in various transmembrane signaling
CC systems. The beta and gamma chains are required for the GTPase
CC activity, for replacement of GDP by GTP, and for G protein-effector
CC interaction. Required for adenylate cyclase activation.
CC {ECO:0000269|PubMed:16884933}.
CC -!- SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:16823372}. Nucleus
CC {ECO:0000269|PubMed:16823372}.
CC -!- SIMILARITY: Belongs to the WD repeat G protein beta family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC37501.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L28061; AAC37501.1; ALT_INIT; Genomic_DNA.
DR EMBL; AF092102; AAD09020.1; -; Genomic_DNA.
DR EMBL; AB004535; BAA21396.1; -; Genomic_DNA.
DR EMBL; CU329671; CAC37497.1; -; Genomic_DNA.
DR PIR; S72457; S72457.
DR PIR; T50474; T50474.
DR RefSeq; NP_595613.1; NM_001021508.2.
DR AlphaFoldDB; Q10282; -.
DR SMR; Q10282; -.
DR BioGRID; 276518; 104.
DR STRING; 4896.SPBC32H8.07.1; -.
DR MaxQB; Q10282; -.
DR PaxDb; Q10282; -.
DR EnsemblFungi; SPBC32H8.07.1; SPBC32H8.07.1:pep; SPBC32H8.07.
DR GeneID; 2539974; -.
DR KEGG; spo:SPBC32H8.07; -.
DR PomBase; SPBC32H8.07; git5.
DR VEuPathDB; FungiDB:SPBC32H8.07; -.
DR eggNOG; KOG0286; Eukaryota.
DR HOGENOM; CLU_000288_57_34_1; -.
DR InParanoid; Q10282; -.
DR OMA; PLDSQWV; -.
DR PhylomeDB; Q10282; -.
DR Reactome; R-SPO-6814122; Cooperation of PDCL (PhLP1) and TRiC/CCT in G-protein beta folding.
DR PRO; PR:Q10282; -.
DR Proteomes; UP000002485; Chromosome II.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; HDA:PomBase.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IDA:PomBase.
DR GO; GO:0005634; C:nucleus; HDA:PomBase.
DR GO; GO:0030159; F:signaling receptor complex adaptor activity; IDA:PomBase.
DR GO; GO:0010619; P:adenylate cyclase-activating glucose-activated G protein-coupled receptor signaling pathway; IMP:PomBase.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IGI:PomBase.
DR GO; GO:0010515; P:negative regulation of induction of conjugation with cellular fusion; IMP:PomBase.
DR GO; GO:0061987; P:negative regulation of transcription from RNA polymerase II promoter by glucose; IMP:PomBase.
DR GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR001632; Gprotein_B.
DR InterPro; IPR016346; Guanine_nucleotide-bd_bsu.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19850; PTHR19850; 1.
DR Pfam; PF00400; WD40; 5.
DR PRINTS; PR00319; GPROTEINB.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 2.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; Sporulation; Transducer;
KW WD repeat.
FT CHAIN 1..305
FT /note="Guanine nucleotide-binding protein subunit beta"
FT /id="PRO_0000127719"
FT REPEAT 19..49
FT /note="WD 1"
FT REPEAT 61..91
FT /note="WD 2"
FT REPEAT 104..133
FT /note="WD 3"
FT REPEAT 145..176
FT /note="WD 4"
FT REPEAT 188..218
FT /note="WD 5"
FT REPEAT 231..260
FT /note="WD 6"
FT REPEAT 272..302
FT /note="WD 7"
SQ SEQUENCE 305 AA; 32828 MW; 7186EB505C68980E CRC64;
MDSGSRVNVN IQGTRVLKNK LGKIPDIDIS TDGKYLLSAS TNDVLLVWDL HTSNKVAFFE
APSVWIMTCA FSPSTKSIAA GGLNNFCVVY DTSVPDADPV ELVGHAGFVS CCKYVDDGHL
LTGSGDKTCM FWDIEQAKAI SVLKGHEMDI VSLDFLPSNP NLFVTGGCDK LAKLWDLRAA
YCCATFPGNT SDINSISFFP SNADFVTGAE DGIARCFDIR ASAEIFQYSS PSSSPINSVL
FSKSGKLLFI AKDKTCEVWD SISSKTITSL TGHENRISSL ALTSDGTMLA TGSWDECVRL
WSSSG
