GBF_DICDI
ID GBF_DICDI Reviewed; 708 AA.
AC P36417; Q54IH5;
DT 01-JUN-1994, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 25-MAY-2022, entry version 97.
DE RecName: Full=G-box-binding factor;
DE Short=GBF;
GN Name=gbfA; ORFNames=DDB_G0288755;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC STRAIN=AX3;
RX PubMed=8125261; DOI=10.1101/gad.8.4.502;
RA Schnitzler G.R., Fischer W.H., Firtel R.A.;
RT "Cloning and characterization of the G-box binding factor, an essential
RT component of the developmental switch between early and late development in
RT Dictyostelium.";
RL Genes Dev. 8:502-514(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: cAMP-responsive transcriptional activator regulating late
CC gene expression. Essential component of the developmental switch
CC between early and late development. Binds to a number of CA/GT-rich
CC gene regulatory elements.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
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DR EMBL; L29075; AAA21021.1; -; mRNA.
DR EMBL; AAFI02000123; EAL63064.1; -; Genomic_DNA.
DR PIR; A53185; A53185.
DR RefSeq; XP_636567.1; XM_631475.1.
DR AlphaFoldDB; P36417; -.
DR STRING; 44689.DDB0191361; -.
DR PaxDb; P36417; -.
DR EnsemblProtists; EAL63064; EAL63064; DDB_G0288755.
DR GeneID; 8626787; -.
DR KEGG; ddi:DDB_G0288755; -.
DR dictyBase; DDB_G0288755; gbfA.
DR eggNOG; ENOG502RSTH; Eukaryota.
DR HOGENOM; CLU_390010_0_0_1; -.
DR InParanoid; P36417; -.
DR OMA; GITKMQQ; -.
DR PRO; PR:P36417; -.
DR Proteomes; UP000002195; Chromosome 5.
DR GO; GO:0005829; C:cytosol; IDA:dictyBase.
DR GO; GO:0005634; C:nucleus; IDA:dictyBase.
DR GO; GO:0003677; F:DNA binding; IDA:dictyBase.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:dictyBase.
DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:dictyBase.
DR GO; GO:0140582; P:adenylate cyclase-activating G protein-coupled cAMP receptor signaling pathway; IMP:dictyBase.
DR GO; GO:0048102; P:autophagic cell death; IMP:dictyBase.
DR GO; GO:0110013; P:positive regulation of aggregation involved in sorocarp development; IMP:dictyBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:dictyBase.
DR GO; GO:1902168; P:response to catechin; IDA:dictyBase.
DR GO; GO:0030587; P:sorocarp development; IEP:dictyBase.
PE 1: Evidence at protein level;
KW Activator; Direct protein sequencing; DNA-binding; Nucleus;
KW Reference proteome; Repeat; Transcription; Transcription regulation.
FT CHAIN 1..708
FT /note="G-box-binding factor"
FT /id="PRO_0000087434"
FT REPEAT 339..368
FT /note="1"
FT REPEAT 481..510
FT /note="2"
FT REGION 1..29
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 123..339
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 511..604
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 123..315
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 513..604
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 597
FT /note="Y -> C (in Ref. 1; AAA21021)"
FT /evidence="ECO:0000305"
FT CONFLICT 613
FT /note="L -> Q (in Ref. 1; AAA21021)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 708 AA; 79312 MW; 3D5B8025C2F540AB CRC64;
MLSTHHHQGN SSSSSSSSSP SQTIGGSDLS NISALPLPLP SIFTTAQNQM NPPILFPPTS
SLLGGSSNTP SFLLPPSSIM SSNVFPSHDG QYPDMPNMVD QYQIHPNQNP HYNYQYQLMF
MQQQAQQNQP PQQNQQQQHH QQQQQQPQHH QQMQQQQHHQ QMQQQQQHHQ QMQQQQHHQQ
MQHHQLQQHQ HQHQQQQQQQ QHQQQHHQQQ QQQQQQHHQQ QQHHQHSQPQ QQHQHNQQQQ
HQHNQQQHQQ QQNQIQMVPQ QPQSLSNSGN NNNNNNNNNN SNNNNNNNNN NNSHQLNNLT
LSQNNTSGSN TPSPSTKGKR KHHETSNSEK KDSSGQTIPK CTRCNEAASW KHDKRRWWCK
ECKKAFTPGI TKMQQVPQQA QLQPLQNHNQ IIPQLWDSQQ NNSSQNTPPT QPQNNMNQIN
HQLLQQQHQQ AQLQAHLNLT ASNQQVPPQL QQQINGGLPN NNNSLITQNT LNSLSTSVSC
PPCPLCRGIS SWKHDKKRYF CKECKKPFTP VGAGLSPSSS PSSPKKKSNI TPITTSSTSS
SSSTPSIINN NNNNNANSSK NNTPKKQLSP PPSVLQSPSS SSISQSPLQL NYQTPTYSPN
PSLPSIGGNL NSLANSIKPD GGILISGLSP PKSSSSLNNL NSFSNTGALL SSNGINLANL
GNPLSQLNKK QKKRSDKDIN DGGVQVLVSC VDTNVNNCSS IIPDGDSW
