GBG12_MOUSE
ID GBG12_MOUSE Reviewed; 72 AA.
AC Q9DAS9; Q544P3;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 161.
DE RecName: Full=Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12;
DE Flags: Precursor;
GN Name=Gng12;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RC TISSUE=Cecum, Colon, Lung, Mammary gland, Placenta, and
RC Sympathetic ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N-3; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=19144319; DOI=10.1016/j.immuni.2008.11.006;
RA Trost M., English L., Lemieux S., Courcelles M., Desjardins M.,
RA Thibault P.;
RT "The phagosomal proteome in interferon-gamma-activated macrophages.";
RL Immunity 30:143-154(2009).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; TYR-42 AND SER-49, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Liver, Lung, Pancreas, Spleen,
RC and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Guanine nucleotide-binding proteins (G proteins) are involved
CC as a modulator or transducer in various transmembrane signaling
CC systems. The beta and gamma chains are required for the GTPase
CC activity, for replacement of GDP by GTP, and for G protein-effector
CC interaction.
CC -!- SUBUNIT: G proteins are composed of 3 units, alpha, beta and gamma.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor
CC {ECO:0000305}; Cytoplasmic side {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the G protein gamma family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK005561; BAB24121.1; -; mRNA.
DR EMBL; AK033422; BAC28280.1; -; mRNA.
DR EMBL; AK148765; BAE28660.1; -; mRNA.
DR EMBL; AK159872; BAE35445.1; -; mRNA.
DR EMBL; AK162342; BAE36863.1; -; mRNA.
DR EMBL; AK166035; BAE38536.1; -; mRNA.
DR EMBL; AK166299; BAE38688.1; -; mRNA.
DR EMBL; BC040685; AAH40685.1; -; mRNA.
DR CCDS; CCDS39502.1; -.
DR RefSeq; NP_001171027.1; NM_001177556.1.
DR RefSeq; NP_001171028.1; NM_001177557.1.
DR RefSeq; NP_001171029.1; NM_001177558.1.
DR RefSeq; NP_001171030.1; NM_001177559.1.
DR RefSeq; NP_001171031.1; NM_001177560.1.
DR RefSeq; NP_079554.1; NM_025278.5.
DR RefSeq; XP_006505605.1; XM_006505542.3.
DR AlphaFoldDB; Q9DAS9; -.
DR SMR; Q9DAS9; -.
DR BioGRID; 199985; 17.
DR IntAct; Q9DAS9; 4.
DR STRING; 10090.ENSMUSP00000109863; -.
DR iPTMnet; Q9DAS9; -.
DR PhosphoSitePlus; Q9DAS9; -.
DR EPD; Q9DAS9; -.
DR jPOST; Q9DAS9; -.
DR MaxQB; Q9DAS9; -.
DR PaxDb; Q9DAS9; -.
DR PeptideAtlas; Q9DAS9; -.
DR PRIDE; Q9DAS9; -.
DR ProteomicsDB; 273418; -.
DR TopDownProteomics; Q9DAS9; -.
DR Antibodypedia; 33413; 66 antibodies from 21 providers.
DR DNASU; 14701; -.
DR Ensembl; ENSMUST00000043148; ENSMUSP00000046557; ENSMUSG00000036402.
DR Ensembl; ENSMUST00000114222; ENSMUSP00000109860; ENSMUSG00000036402.
DR Ensembl; ENSMUST00000114224; ENSMUSP00000109862; ENSMUSG00000036402.
DR Ensembl; ENSMUST00000114225; ENSMUSP00000109863; ENSMUSG00000036402.
DR Ensembl; ENSMUST00000114226; ENSMUSP00000109864; ENSMUSG00000036402.
DR Ensembl; ENSMUST00000114227; ENSMUSP00000109865; ENSMUSG00000036402.
DR Ensembl; ENSMUST00000114228; ENSMUSP00000109866; ENSMUSG00000036402.
DR GeneID; 14701; -.
DR KEGG; mmu:14701; -.
DR UCSC; uc009cew.2; mouse.
DR CTD; 55970; -.
DR MGI; MGI:1336171; Gng12.
DR VEuPathDB; HostDB:ENSMUSG00000036402; -.
DR eggNOG; KOG4119; Eukaryota.
DR GeneTree; ENSGT01050000244858; -.
DR HOGENOM; CLU_168377_3_1_1; -.
DR InParanoid; Q9DAS9; -.
DR OMA; MDIMASN; -.
DR OrthoDB; 1581168at2759; -.
DR PhylomeDB; Q9DAS9; -.
DR TreeFam; TF319909; -.
DR BioGRID-ORCS; 14701; 5 hits in 72 CRISPR screens.
DR ChiTaRS; Gng12; mouse.
DR PRO; PR:Q9DAS9; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q9DAS9; protein.
DR Bgee; ENSMUSG00000036402; Expressed in gastrula and 269 other tissues.
DR ExpressionAtlas; Q9DAS9; baseline and differential.
DR Genevisible; Q9DAS9; MM.
DR GO; GO:0005884; C:actin filament; ISO:MGI.
DR GO; GO:0005834; C:heterotrimeric G-protein complex; IBA:GO_Central.
DR GO; GO:0031681; F:G-protein beta-subunit binding; IBA:GO_Central.
DR GO; GO:0030165; F:PDZ domain binding; ISO:MGI.
DR GO; GO:0042301; F:phosphate ion binding; ISO:MGI.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IBA:GO_Central.
DR CDD; cd00068; GGL; 1.
DR Gene3D; 4.10.260.10; -; 1.
DR InterPro; IPR015898; G-protein_gamma-like_dom.
DR InterPro; IPR036284; GGL_sf.
DR InterPro; IPR001770; Gprotein-gamma.
DR PANTHER; PTHR13809; PTHR13809; 1.
DR Pfam; PF00631; G-gamma; 1.
DR PRINTS; PR00321; GPROTEING.
DR SMART; SM00224; GGL; 1.
DR SUPFAM; SSF48670; SSF48670; 1.
DR PROSITE; PS50058; G_PROTEIN_GAMMA; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell membrane; Lipoprotein; Membrane; Methylation;
KW Phosphoprotein; Prenylation; Reference proteome; Transducer.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q28024"
FT CHAIN 2..69
FT /note="Guanine nucleotide-binding protein G(I)/G(S)/G(O)
FT subunit gamma-12"
FT /id="PRO_0000012669"
FT PROPEP 70..72
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000012670"
FT MOD_RES 2
FT /note="N-acetylserine"
FT /evidence="ECO:0000250|UniProtKB:Q28024"
FT MOD_RES 10
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 26
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:19144319"
FT MOD_RES 42
FT /note="Phosphotyrosine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 49
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 69
FT /note="Cysteine methyl ester"
FT /evidence="ECO:0000250"
FT LIPID 69
FT /note="S-geranylgeranyl cysteine"
FT /evidence="ECO:0000250"
SQ SEQUENCE 72 AA; 7997 MW; A4D56F3BD697E7B4 CRC64;
MSSKTASTNS IAQARRTVQQ LRLEASIERI KVSKASADLM SYCEEHARSD PLLMGIPTSE
NPFKDKKTCI IL
