GBLP_CAEEL
ID GBLP_CAEEL Reviewed; 325 AA.
AC Q21215;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 03-AUG-2022, entry version 160.
DE RecName: Full=Guanine nucleotide-binding protein subunit beta-2-like 1;
GN Name=rack-1; ORFNames=K04D7.1;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP PROTEIN SEQUENCE OF 2-14.
RC STRAIN=Bristol N2;
RX PubMed=9150941; DOI=10.1002/elps.1150180337;
RA Bini L., Heid H., Liberatori S., Geier G., Pallini V., Zwilling R.;
RT "Two-dimensional gel electrophoresis of Caenorhabditis elegans homogenates
RT and identification of protein spots by microsequencing.";
RL Electrophoresis 18:557-562(1997).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=19380113; DOI=10.1016/j.chom.2009.03.006;
RA Ziegler K., Kurz C.L., Cypowyj S., Couillault C., Pophillat M., Pujol N.,
RA Ewbank J.J.;
RT "Antifungal innate immunity in C. elegans: PKCdelta links G protein
RT signaling and a conserved p38 MAPK cascade.";
RL Cell Host Microbe 5:341-352(2009).
CC -!- FUNCTION: Required for the expression of antimicrobial peptide nlp-29
CC in response to fungal infection or physical injury.
CC {ECO:0000269|PubMed:19380113}.
CC -!- INTERACTION:
CC Q21215; G5EBY5: sipa-1; NbExp=2; IntAct=EBI-315497, EBI-314833;
CC Q21215; Q95QM5: unc-115; NbExp=2; IntAct=EBI-315497, EBI-4405677;
CC -!- DISRUPTION PHENOTYPE: RNAi-mediated knockdown causes a complete loss of
CC nlp-29 expression following fungal infection by D.coniospora and a
CC partial reduction following physical injury.
CC {ECO:0000269|PubMed:19380113}.
CC -!- SIMILARITY: Belongs to the WD repeat G protein beta family. Ribosomal
CC protein RACK1 subfamily. {ECO:0000305}.
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DR EMBL; Z69664; CAA93514.1; -; Genomic_DNA.
DR PIR; T23309; T23309.
DR RefSeq; NP_501859.1; NM_069458.4.
DR AlphaFoldDB; Q21215; -.
DR SMR; Q21215; -.
DR BioGRID; 42997; 58.
DR DIP; DIP-26516N; -.
DR IntAct; Q21215; 6.
DR MINT; Q21215; -.
DR STRING; 6239.K04D7.1.3; -.
DR iPTMnet; Q21215; -.
DR World-2DPAGE; 0020:Q21215; -.
DR EPD; Q21215; -.
DR PaxDb; Q21215; -.
DR PeptideAtlas; Q21215; -.
DR EnsemblMetazoa; K04D7.1.1; K04D7.1.1; WBGene00010556.
DR EnsemblMetazoa; K04D7.1.2; K04D7.1.2; WBGene00010556.
DR EnsemblMetazoa; K04D7.1.3; K04D7.1.3; WBGene00010556.
DR GeneID; 177895; -.
DR KEGG; cel:CELE_K04D7.1; -.
DR UCSC; K04D7.1.1; c. elegans.
DR CTD; 177895; -.
DR WormBase; K04D7.1; CE06090; WBGene00010556; rack-1.
DR eggNOG; KOG0279; Eukaryota.
DR GeneTree; ENSGT00940000154461; -.
DR HOGENOM; CLU_000288_57_7_1; -.
DR InParanoid; Q21215; -.
DR OMA; CKAMLWD; -.
DR OrthoDB; 805365at2759; -.
DR PhylomeDB; Q21215; -.
DR SignaLink; Q21215; -.
DR PRO; PR:Q21215; -.
DR Proteomes; UP000001940; Chromosome IV.
DR Bgee; WBGene00010556; Expressed in larva and 4 other tissues.
DR GO; GO:0030424; C:axon; IDA:WormBase.
DR GO; GO:0044297; C:cell body; IDA:WormBase.
DR GO; GO:0005813; C:centrosome; IDA:WormBase.
DR GO; GO:0005737; C:cytoplasm; IDA:WormBase.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0030426; C:growth cone; IDA:WormBase.
DR GO; GO:0000776; C:kinetochore; IDA:WormBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:WormBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR GO; GO:0005080; F:protein kinase C binding; IBA:GO_Central.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR GO; GO:0051304; P:chromosome separation; IMP:WormBase.
DR GO; GO:0050832; P:defense response to fungus; IMP:WormBase.
DR GO; GO:0009792; P:embryo development ending in birth or egg hatching; IMP:WormBase.
DR GO; GO:0000281; P:mitotic cytokinesis; IMP:WormBase.
DR GO; GO:0008045; P:motor neuron axon guidance; IMP:WormBase.
DR GO; GO:0040038; P:polar body extrusion after meiotic divisions; IMP:WormBase.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; IBA:GO_Central.
DR GO; GO:0033365; P:protein localization to organelle; IMP:WormBase.
DR GO; GO:0030334; P:regulation of cell migration; IMP:WormBase.
DR GO; GO:0072344; P:rescue of stalled ribosome; IBA:GO_Central.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR045223; Asc1/RACK1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19868; PTHR19868; 1.
DR Pfam; PF00400; WD40; 7.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 5.
DR PROSITE; PS50082; WD_REPEATS_2; 5.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Reference proteome; Repeat; Ribonucleoprotein;
KW Ribosomal protein; WD repeat.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:9150941"
FT CHAIN 2..325
FT /note="Guanine nucleotide-binding protein subunit beta-2-
FT like 1"
FT /id="PRO_0000127739"
FT REPEAT 5..48
FT /note="WD 1"
FT REPEAT 58..99
FT /note="WD 2"
FT REPEAT 100..141
FT /note="WD 3"
FT REPEAT 143..186
FT /note="WD 4"
FT REPEAT 187..227
FT /note="WD 5"
FT REPEAT 228..268
FT /note="WD 6"
FT REPEAT 269..320
FT /note="WD 7"
SQ SEQUENCE 325 AA; 35830 MW; C50669418DBCD0A5 CRC64;
MVQEQMKLTG TLEGHTGWVT QIATYTRNDK TTVLSSSRDK TILVWDVDSV APVDEGPIGR
PVRSLTGHNH FVSDVVISSD GQFALSGSWD KTLRLWDLNQ GVSTRQFISH TKDVLSVAFS
ADNRQIVSGS RDKSIKLWNT LAQCKYTITD DCHTDWVSTV RFSPSNRDPV IVSAGWDKVV
KVWNLGNCRL KTNHIGHTGY VNTVTVSPDG SLCASGGKDG QAMLWDLNEG KHLYTLPGND
VINAMSFSPN RYWLCAAVGS SIKIWDLEDK KEIEELKPEI ASSGSSRGSS PQCISLAWSQ
DGQTLFAGYT DNIIRVYQVS IRASN