GBLP_CANAL
ID GBLP_CANAL Reviewed; 317 AA.
AC P83774; A0A1D8PQU0; Q3MPL4; Q59S46;
DT 04-JAN-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 2.
DT 03-AUG-2022, entry version 84.
DE RecName: Full=Guanine nucleotide-binding protein subunit beta-like protein;
DE AltName: Full=Cytoplasmic antigenic protein 1;
GN Name=ASC1; OrderedLocusNames=CAALFM_C701250WA;
GN ORFNames=CaJ7.0140, CaO19.6906;
OS Candida albicans (strain SC5314 / ATCC MYA-2876) (Yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Debaryomycetaceae; Candida/Lodderomyces clade; Candida.
OX NCBI_TaxID=237561;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15937140; DOI=10.1534/genetics.104.034652;
RA Chibana H., Oka N., Nakayama H., Aoyama T., Magee B.B., Magee P.T.,
RA Mikami Y.;
RT "Sequence finishing and gene mapping for Candida albicans chromosome 7 and
RT syntenic analysis against the Saccharomyces cerevisiae genome.";
RL Genetics 170:1525-1537(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=15123810; DOI=10.1073/pnas.0401648101;
RA Jones T., Federspiel N.A., Chibana H., Dungan J., Kalman S., Magee B.B.,
RA Newport G., Thorstenson Y.R., Agabian N., Magee P.T., Davis R.W.,
RA Scherer S.;
RT "The diploid genome sequence of Candida albicans.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:7329-7334(2004).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=17419877; DOI=10.1186/gb-2007-8-4-r52;
RA van het Hoog M., Rast T.J., Martchenko M., Grindle S., Dignard D.,
RA Hogues H., Cuomo C., Berriman M., Scherer S., Magee B.B., Whiteway M.,
RA Chibana H., Nantel A., Magee P.T.;
RT "Assembly of the Candida albicans genome into sixteen supercontigs aligned
RT on the eight chromosomes.";
RL Genome Biol. 8:RESEARCH52.1-RESEARCH52.12(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC STRAIN=SC5314 / ATCC MYA-2876;
RX PubMed=24025428; DOI=10.1186/gb-2013-14-9-r97;
RA Muzzey D., Schwartz K., Weissman J.S., Sherlock G.;
RT "Assembly of a phased diploid Candida albicans genome facilitates allele-
RT specific measurements and provides a simple model for repeat and indel
RT structure.";
RL Genome Biol. 14:RESEARCH97.1-RESEARCH97.14(2013).
RN [5]
RP PROTEIN SEQUENCE OF 47-59; 92-103; 159-173 AND 235-246, SUBCELLULAR
RP LOCATION, AND ANTIGENICITY.
RC STRAIN=SC5314 / ATCC MYA-2876; TISSUE=Protoplast;
RX PubMed=15378761; DOI=10.1002/pmic.200400903;
RA Pitarch A., Abian J., Carrascal M., Sanchez M., Nombela C., Gil C.;
RT "Proteomics-based identification of novel Candida albicans antigens for
RT diagnosis of systemic candidiasis in patients with underlying hematological
RT malignancies.";
RL Proteomics 4:3084-3106(2004).
RN [6]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=20929924; DOI=10.1093/abbs/gmq093;
RA Liu X., Nie X., Ding Y., Chen J.;
RT "Asc1, a WD-repeat protein, is required for hyphal development and
RT virulence in Candida albicans.";
RL Acta Biochim. Biophys. Sin. 42:793-800(2010).
CC -!- FUNCTION: Located at the head of the 40S ribosomal subunit in the
CC vicinity of the mRNA exit channel, it serves as a scaffold protein that
CC can recruit other proteins to the ribosome. Involved in the negative
CC regulation of translation of a specific subset of proteins (By
CC similarity). Plays a role in morphogenesis and pathogenesis.
CC {ECO:0000250, ECO:0000269|PubMed:20929924}.
CC -!- SUBUNIT: Component of the small (40S) ribosomal subunit. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:15378761}.
CC -!- DISRUPTION PHENOTYPE: Causes defects in hyphal development under hypha-
CC inducing conditions and attenuates virulence in a mouse model of
CC systemic infection. {ECO:0000269|PubMed:20929924}.
CC -!- MISCELLANEOUS: Has antigenic properties. Elicits a specific immune
CC response in systemic candidiasis human patients undergoing malignant
CC hematological disorders.
CC -!- SIMILARITY: Belongs to the WD repeat G protein beta family. Ribosomal
CC protein RACK1 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAE44646.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AP006852; BAE44646.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP017629; AOW30494.1; -; Genomic_DNA.
DR RefSeq; XP_019331027.1; XM_019475482.1.
DR AlphaFoldDB; P83774; -.
DR SMR; P83774; -.
DR BioGRID; 1229000; 4.
DR STRING; 237561.P83774; -.
DR COMPLUYEAST-2DPAGE; P83774; -.
DR PRIDE; P83774; -.
DR GeneID; 3645907; -.
DR KEGG; cal:CAALFM_C701250WA; -.
DR CGD; CAL0000180554; ASC1.
DR VEuPathDB; FungiDB:C7_01250W_A; -.
DR eggNOG; KOG0279; Eukaryota.
DR HOGENOM; CLU_000288_57_7_1; -.
DR InParanoid; P83774; -.
DR OMA; CKAMLWD; -.
DR OrthoDB; 805365at2759; -.
DR PHI-base; PHI:3514; -.
DR Proteomes; UP000000559; Chromosome 7.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0022627; C:cytosolic small ribosomal subunit; IEA:EnsemblFungi.
DR GO; GO:1903561; C:extracellular vesicle; IDA:CGD.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001965; F:G-protein alpha-subunit binding; IEA:EnsemblFungi.
DR GO; GO:0005092; F:GDP-dissociation inhibitor activity; IGI:CGD.
DR GO; GO:0005080; F:protein kinase C binding; IBA:GO_Central.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR GO; GO:0007155; P:cell adhesion; IMP:CGD.
DR GO; GO:0036244; P:cellular response to neutral pH; IMP:CGD.
DR GO; GO:0009267; P:cellular response to starvation; IMP:CGD.
DR GO; GO:0030447; P:filamentous growth; IMP:CGD.
DR GO; GO:0036180; P:filamentous growth of a population of unicellular organisms in response to biotic stimulus; IMP:CGD.
DR GO; GO:0036178; P:filamentous growth of a population of unicellular organisms in response to neutral pH; IMP:CGD.
DR GO; GO:0036170; P:filamentous growth of a population of unicellular organisms in response to starvation; IMP:CGD.
DR GO; GO:0007186; P:G protein-coupled receptor signaling pathway; IGI:CGD.
DR GO; GO:0140469; P:GCN2-mediated signaling; IEA:EnsemblFungi.
DR GO; GO:0001403; P:invasive growth in response to glucose limitation; IMP:CGD.
DR GO; GO:0061157; P:mRNA destabilization; IEA:EnsemblFungi.
DR GO; GO:1903138; P:negative regulation of cell wall integrity MAPK cascade; IEA:EnsemblFungi.
DR GO; GO:1902660; P:negative regulation of glucose mediated signaling pathway; IEA:EnsemblFungi.
DR GO; GO:1903753; P:negative regulation of p38MAPK cascade; IEA:EnsemblFungi.
DR GO; GO:2001125; P:negative regulation of translational frameshifting; IEA:EnsemblFungi.
DR GO; GO:0010508; P:positive regulation of autophagy; IEA:EnsemblFungi.
DR GO; GO:0031139; P:positive regulation of conjugation with cellular fusion; IEA:EnsemblFungi.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; IBA:GO_Central.
DR GO; GO:0008104; P:protein localization; IEA:EnsemblFungi.
DR GO; GO:2000765; P:regulation of cytoplasmic translation; IEA:EnsemblFungi.
DR GO; GO:0032995; P:regulation of fungal-type cell wall biogenesis; IEA:EnsemblFungi.
DR GO; GO:0072344; P:rescue of stalled ribosome; IBA:GO_Central.
DR GO; GO:1990116; P:ribosome-associated ubiquitin-dependent protein catabolic process; IEA:EnsemblFungi.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR045223; Asc1/RACK1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19868; PTHR19868; 1.
DR Pfam; PF00400; WD40; 6.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 4.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Reference proteome; Repeat;
KW Ribonucleoprotein; Ribosomal protein; WD repeat.
FT CHAIN 1..317
FT /note="Guanine nucleotide-binding protein subunit beta-like
FT protein"
FT /id="PRO_0000089300"
FT REPEAT 15..55
FT /note="WD 1"
FT REPEAT 64..103
FT /note="WD 2"
FT REPEAT 106..146
FT /note="WD 3"
FT REPEAT 148..188
FT /note="WD 4"
FT REPEAT 191..230
FT /note="WD 5"
FT REPEAT 232..272
FT /note="WD 6"
FT REPEAT 281..317
FT /note="WD 7"
SQ SEQUENCE 317 AA; 34555 MW; C31FFE7FFAB153B8 CRC64;
MADQEVLVLR GTLEGHNGWV TSLATTPAHP DLLLSGSRDK TLIKWKLTGG EDNQYGIPKK
SFKGHSHIVQ DVTISADGAY ALSASWDRTL RLWDLETGET TQRFVGHKGD VLSVSIAKNL
RQIVSASRDK TVKVWNTIGE CMATLTGHND WVSAVRISPS DQSSTVISAS WDKTVKSWDL
ADYSVNADFI GHTGYISCIT LSPDGSLCAS AGKDGVIILW DLNKNKTLYT LEAKAEVHAL
AFSPNRYWLA AATTSGIKIF KLQERSLLDE LKPEFAVGAT AKDPEAISLA WSADGQNLFA
GYTDNVIRVW QVMTPSA
