GBLP_DROME
ID GBLP_DROME Reviewed; 318 AA.
AC O18640; Q9VLW5;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Guanine nucleotide-binding protein subunit beta-like protein;
DE AltName: Full=Receptor of activated protein kinase C homolog;
GN Name=Rack1; ORFNames=CG7111;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=Oregon-R;
RX PubMed=9296523; DOI=10.1016/s0167-4889(97)00079-7;
RA Vani K., Yang G., Mohler J.;
RT "Isolation and cloning of a Drosophila homolog to the mammalian RACK1 gene,
RT implicated in PKC-mediated signalling.";
RL Biochim. Biophys. Acta 1358:67-71(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP STRUCTURE BY ELECTRON MICROSCOPY (6.0 ANGSTROMS) IN COMPLEX WITH THE 80S
RP RIBOSOME, AND SUBUNIT.
RX PubMed=23636399; DOI=10.1038/nature12104;
RA Anger A.M., Armache J.P., Berninghausen O., Habeck M., Subklewe M.,
RA Wilson D.N., Beckmann R.;
RT "Structures of the human and Drosophila 80S ribosome.";
RL Nature 497:80-85(2013).
CC -!- FUNCTION: Involved in the recruitment, assembly and/or regulation of a
CC variety of signaling molecules. Interacts with a wide variety of
CC proteins and plays a role in many cellular processes (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Interacts with the 80S ribosome.
CC {ECO:0000269|PubMed:23636399}.
CC -!- TISSUE SPECIFICITY: Highest expression in the mesodermal and endodermal
CC lineages. {ECO:0000269|PubMed:9296523}.
CC -!- SIMILARITY: Belongs to the WD repeat G protein beta family. Ribosomal
CC protein RACK1 subfamily. {ECO:0000305}.
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DR EMBL; U96491; AAB72148.1; -; mRNA.
DR EMBL; AE014134; AAF52566.1; -; Genomic_DNA.
DR EMBL; AY071661; AAL49283.1; -; mRNA.
DR RefSeq; NP_001260218.1; NM_001273289.1.
DR RefSeq; NP_001285723.1; NM_001298794.1.
DR RefSeq; NP_477269.1; NM_057921.4.
DR PDB; 4V6W; EM; 6.00 A; Ag=1-318.
DR PDB; 6XU6; EM; 3.50 A; Ag=1-318.
DR PDB; 6XU7; EM; 4.90 A; Ag=1-318.
DR PDB; 6XU8; EM; 3.00 A; Ag=1-318.
DR PDBsum; 4V6W; -.
DR PDBsum; 6XU6; -.
DR PDBsum; 6XU7; -.
DR PDBsum; 6XU8; -.
DR AlphaFoldDB; O18640; -.
DR SMR; O18640; -.
DR BioGRID; 60209; 134.
DR DIP; DIP-17169N; -.
DR IntAct; O18640; 29.
DR STRING; 7227.FBpp0303680; -.
DR PaxDb; O18640; -.
DR PRIDE; O18640; -.
DR EnsemblMetazoa; FBtr0079565; FBpp0079187; FBgn0020618.
DR EnsemblMetazoa; FBtr0331238; FBpp0303680; FBgn0020618.
DR EnsemblMetazoa; FBtr0345448; FBpp0311572; FBgn0020618.
DR GeneID; 34070; -.
DR KEGG; dme:Dmel_CG7111; -.
DR CTD; 10399; -.
DR FlyBase; FBgn0020618; Rack1.
DR VEuPathDB; VectorBase:FBgn0020618; -.
DR eggNOG; KOG0279; Eukaryota.
DR GeneTree; ENSGT00940000154461; -.
DR HOGENOM; CLU_000288_57_7_1; -.
DR InParanoid; O18640; -.
DR OMA; CKAMLWD; -.
DR OrthoDB; 805365at2759; -.
DR PhylomeDB; O18640; -.
DR SignaLink; O18640; -.
DR BioGRID-ORCS; 34070; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Rack1; fly.
DR GenomeRNAi; 34070; -.
DR PRO; PR:O18640; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0020618; Expressed in wing disc and 24 other tissues.
DR ExpressionAtlas; O18640; baseline and differential.
DR Genevisible; O18640; DM.
DR GO; GO:0005776; C:autophagosome; IDA:FlyBase.
DR GO; GO:0005737; C:cytoplasm; IDA:FlyBase.
DR GO; GO:0005829; C:cytosol; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0015935; C:small ribosomal subunit; IEA:InterPro.
DR GO; GO:0005080; F:protein kinase C binding; IBA:GO_Central.
DR GO; GO:0030674; F:protein-macromolecule adaptor activity; IPI:FlyBase.
DR GO; GO:0043022; F:ribosome binding; IBA:GO_Central.
DR GO; GO:0009267; P:cellular response to starvation; IMP:FlyBase.
DR GO; GO:0042335; P:cuticle development; IMP:FlyBase.
DR GO; GO:0075522; P:IRES-dependent viral translational initiation; IMP:FlyBase.
DR GO; GO:0007626; P:locomotory behavior; IMP:FlyBase.
DR GO; GO:0046716; P:muscle cell cellular homeostasis; IGI:FlyBase.
DR GO; GO:0001933; P:negative regulation of protein phosphorylation; IMP:UniProtKB.
DR GO; GO:0045879; P:negative regulation of smoothened signaling pathway; IMP:FlyBase.
DR GO; GO:0048477; P:oogenesis; IMP:FlyBase.
DR GO; GO:0018991; P:oviposition; IMP:FlyBase.
DR GO; GO:0044829; P:positive regulation by host of viral genome replication; IMP:FlyBase.
DR GO; GO:0030838; P:positive regulation of actin filament polymerization; IMP:UniProtKB.
DR GO; GO:0045725; P:positive regulation of glycogen biosynthetic process; IMP:FlyBase.
DR GO; GO:1903003; P:positive regulation of protein deubiquitination; IDA:FlyBase.
DR GO; GO:0001934; P:positive regulation of protein phosphorylation; IBA:GO_Central.
DR GO; GO:0031398; P:positive regulation of protein ubiquitination; IGI:FlyBase.
DR GO; GO:0016243; P:regulation of autophagosome size; IMP:FlyBase.
DR GO; GO:0072344; P:rescue of stalled ribosome; IBA:GO_Central.
DR GO; GO:0035220; P:wing disc development; IMP:FlyBase.
DR Gene3D; 2.130.10.10; -; 1.
DR InterPro; IPR045223; Asc1/RACK1.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR001680; WD40_repeat.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR PANTHER; PTHR19868; PTHR19868; 1.
DR Pfam; PF00400; WD40; 7.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 7.
DR SUPFAM; SSF50978; SSF50978; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 4.
DR PROSITE; PS50082; WD_REPEATS_2; 6.
DR PROSITE; PS50294; WD_REPEATS_REGION; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Repeat; Ribonucleoprotein;
KW Ribosomal protein; WD repeat.
FT CHAIN 1..318
FT /note="Guanine nucleotide-binding protein subunit beta-like
FT protein"
FT /id="PRO_0000127740"
FT REPEAT 13..44
FT /note="WD 1"
FT REPEAT 62..92
FT /note="WD 2"
FT REPEAT 104..134
FT /note="WD 3"
FT REPEAT 147..179
FT /note="WD 4"
FT REPEAT 191..221
FT /note="WD 5"
FT REPEAT 232..261
FT /note="WD 6"
FT REPEAT 282..312
FT /note="WD 7"
FT CONFLICT 104
FT /note="G -> R (in Ref. 1; AAB72148)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 318 AA; 35618 MW; 202930BE51AEE822 CRC64;
MSETLQLRGT LIGHNGWVTQ IATNPKDPDT IISASRDKTL IVWKLTRDED TNYGYPQKRL
YGHSHFISDV VLSSDGNYAL SGSWDQTLRL WDLAAGKTTR RFEGHTKDVL SVAFSADNRQ
IVSGSRDKTI KLWNTLAECK FTIQEDGHTD WVSCVRFSPN HSNPIIVSCG WDRTVKVWNL
ANCKLKNNHH GHNGYLNTVT VSPDGSLCTS GGKDSKALLW DLNDGKNLYT LEHNDIINAL
CFSPNRYWLC VAYGPSIKIW DLACKKTVEE LRPEVVSPTS KADQPQCLSL AWSTDGQTLF
AGYSDNTIRV WQVSVSAH
