ID   GBPA_VIBCH              Reviewed;         485 AA.
AC   Q9KLD5;
DT   04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   03-AUG-2022, entry version 91.
DE   RecName: Full=GlcNAc-binding protein A;
DE   Flags: Precursor;
GN   Name=gbpA; OrderedLocusNames=VC_A0811;
OS   Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=243277;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX   PubMed=10952301; DOI=10.1038/35020000;
RA   Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA   Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA   Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA   Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA   Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA   Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT   "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT   cholerae.";
RL   Nature 406:477-483(2000).
RN   [2]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=Classical CG842 / Serotype O1;
RX   PubMed=16341015; DOI=10.1038/nature04249;
RA   Kirn T.J., Jude B.A., Taylor R.K.;
RT   "A colonization factor links Vibrio cholerae environmental survival and
RT   human infection.";
RL   Nature 438:863-866(2005).
CC   -!- FUNCTION: Probably interacts with GlcNAc residues. May promote
CC       attachment to both epithelial cell surfaces and chitin. This function
CC       enhances bacterial colonization in the gastrointestinal tract and may
CC       also be important in the environment by augment colonization of
CC       chitinous structures, leading to improved survival. Promotes bacterial
CC       attachment to, and colonization of, zooplankton in the aquatic
CC       ecosystem. {ECO:0000269|PubMed:16341015}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:16341015}.
CC       Note=Secreted via the type II secretion pathway EPS (extracellular
CC       protein secretion).
CC   -!- SIMILARITY: Belongs to the GbpA family. {ECO:0000305}.
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DR   EMBL; AE003853; AAF96709.1; -; Genomic_DNA.
DR   PIR; A82414; A82414.
DR   RefSeq; NP_233197.1; NC_002506.1.
DR   RefSeq; WP_000744635.1; NZ_LT906615.1.
DR   PDB; 2XWX; X-ray; 1.80 A; A/B=24-414.
DR   PDBsum; 2XWX; -.
DR   AlphaFoldDB; Q9KLD5; -.
DR   SMR; Q9KLD5; -.
DR   STRING; 243277.VC_A0811; -.
DR   CAZy; AA10; Auxiliary Activities 10.
DR   CAZy; CBM73; Carbohydrate-Binding Module Family 73.
DR   PRIDE; Q9KLD5; -.
DR   DNASU; 2611865; -.
DR   EnsemblBacteria; AAF96709; AAF96709; VC_A0811.
DR   GeneID; 57742190; -.
DR   KEGG; vch:VC_A0811; -.
DR   PATRIC; fig|243277.26.peg.3431; -.
DR   eggNOG; COG3397; Bacteria.
DR   HOGENOM; CLU_039396_2_0_6; -.
DR   OMA; WTFTANH; -.
DR   BioCyc; VCHO:VCA0811-MON; -.
DR   BRENDA; 1.14.99.54; 15003.
DR   Proteomes; UP000000584; Chromosome 2.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0008061; F:chitin binding; IEA:UniProtKB-UniRule.
DR   HAMAP; MF_01905; GbpA; 1.
DR   InterPro; IPR004302; Cellulose/chitin-bd_N.
DR   InterPro; IPR041029; GbpA_2.
DR   InterPro; IPR020879; GlcNAc-bd_A.
DR   InterPro; IPR014756; Ig_E-set.
DR   Pfam; PF18416; GbpA_2; 1.
DR   Pfam; PF03067; LPMO_10; 1.
DR   SUPFAM; SSF81296; SSF81296; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Chitin-binding; Reference proteome; Secreted; Signal.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..485
FT                   /note="GlcNAc-binding protein A"
FT                   /id="PRO_0000229723"
FT   DOMAIN          24..201
FT                   /note="Chitin-binding type-4"
FT   DOMAIN          437..478
FT                   /note="Chitin-binding type-3"
FT   STRAND          25..28
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          30..32
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   HELIX           40..43
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   TURN            47..49
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   HELIX           57..61
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   HELIX           63..65
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          67..70
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   TURN            72..74
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          75..77
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   TURN            82..86
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   HELIX           88..94
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          104..106
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          108..119
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          123..131
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   HELIX           143..145
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          151..159
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          163..171
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          176..201
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          211..217
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          228..235
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   HELIX           241..243
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          246..248
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   HELIX           252..255
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   HELIX           257..271
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          273..281
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          284..286
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          292..297
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          304..310
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          319..323
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          327..330
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          337..355
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          361..369
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          374..380
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          386..397
FT                   /evidence="ECO:0007829|PDB:2XWX"
FT   STRAND          402..413
FT                   /evidence="ECO:0007829|PDB:2XWX"
SQ   SEQUENCE   485 AA;  53628 MW;  975D21307B02BCE4 CRC64;
     MKKQPKMTAI ALILSGISGL AYGHGYVSAV ENGVAEGRVT LCKFAANGTG EKNTHCGAIQ
     YEPQSVEGPD GFPVTGPRDG KIASAESALA AALDEQTADR WVKRPIQAGP QTFEWTFTAN
     HVTKDWKYYI TKPNWNPNQP LSRDAFDLNP FCVVEGNMVQ PPKRVSHECI VPEREGYQVI
     LAVWDVGDTA ASFYNVIDVK FDGNGPVLPD WNPAGQIIPS MDLSIGDTVY TRVFDNDGEN
     PAYRTELKID SETLTKANQW SYALATKINQ TQKQQRAGQL NGDQFVPVYG TNPIYLKEGS
     GLKSVEIGYQ IEAPQPEYSL TVSGLAKEYE IGEQPIQLDL TLEAQGEMSA ELTVYNHHQK
     PLASWSQAMT DGELKSITLE LSEAKAGHHM LVSRIKDRDG NLQDQQTLDF MLVEPQTPPT
     PGDYDFVFPN GLKEYVAGTK VLASDGAIYQ CKPWPYSGYC QQWTSNATQY QPGTGSHWEM
     AWDKR