位置:首页 > 蛋白库 > GBPD_DICDI
GBPD_DICDI
ID   GBPD_DICDI              Reviewed;        1312 AA.
AC   Q54S40; Q8MVR0;
DT   25-NOV-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   03-AUG-2022, entry version 103.
DE   RecName: Full=Cyclic GMP-binding protein D;
DE   AltName: Full=Guanine nucleotide exchange factor for rap1;
DE   AltName: Full=Ras guanine nucleotide exchange factor U;
DE   AltName: Full=RasGEF domain-containing protein U;
GN   Name=gbpD; Synonyms=gefU, rapgef; ORFNames=DDB_G0282373;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX   PubMed=12011437; DOI=10.1073/pnas.102167299;
RA   Goldberg J.M., Bosgraaf L., Van Haastert P.J.M., Smith J.L.;
RT   "Identification of four candidate cGMP targets in Dictyostelium.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:6749-6754(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [3]
RP   FUNCTION.
RX   PubMed=12198158; DOI=10.1093/emboj/cdf438;
RA   Bosgraaf L., Russcher H., Smith J.L., Wessels D., Soll D.R.,
RA   Van Haastert P.J.M.;
RT   "A novel cGMP signalling pathway mediating myosin phosphorylation and
RT   chemotaxis in Dictyostelium.";
RL   EMBO J. 21:4560-4570(2002).
RN   [4]
RP   DEVELOPMENTAL STAGE.
RX   PubMed=16086850; DOI=10.1186/gb-2005-6-8-r68;
RA   Wilkins A., Szafranski K., Fraser D.J., Bakthavatsalam D., Mueller R.,
RA   Fisher P.R., Gloeckner G., Eichinger L., Noegel A.A., Insall R.H.;
RT   "The Dictyostelium genome encodes numerous RasGEFs with multiple biological
RT   roles.";
RL   Genome Biol. 6:R68.1-R68.12(2005).
RN   [5]
RP   FUNCTION.
RX   PubMed=15827084; DOI=10.1242/jcs.02317;
RA   Bosgraaf L., Waijer A., Engel R., Visser A.J.W.G., Wessels D., Soll D.,
RA   van Haastert P.J.M.;
RT   "RasGEF-containing proteins GbpC and GbpD have differential effects on cell
RT   polarity and chemotaxis in Dictyostelium.";
RL   J. Cell Sci. 118:1899-1910(2005).
CC   -!- FUNCTION: Promotes the exchange of Ras-bound GDP by GTP (By
CC       similarity). Induces the formation of substrate-attached pseudopodia,
CC       that leads to increased adhesion and thereby negatively influencing
CC       cell speed and polarity. {ECO:0000250, ECO:0000269|PubMed:12198158,
CC       ECO:0000269|PubMed:15827084}.
CC   -!- DEVELOPMENTAL STAGE: Increases after starvation is initiated with a
CC       peak between 8-12 hours of starvation. {ECO:0000269|PubMed:12011437,
CC       ECO:0000269|PubMed:16086850}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AF481924; AAM34042.1; -; Genomic_DNA.
DR   EMBL; AAFI02000047; EAL66046.1; -; Genomic_DNA.
DR   RefSeq; XP_640199.1; XM_635107.1.
DR   AlphaFoldDB; Q54S40; -.
DR   STRING; 44689.DDB0215332; -.
DR   PaxDb; Q54S40; -.
DR   EnsemblProtists; EAL66046; EAL66046; DDB_G0282373.
DR   GeneID; 8623739; -.
DR   KEGG; ddi:DDB_G0282373; -.
DR   dictyBase; DDB_G0282373; gbpD.
DR   eggNOG; KOG3417; Eukaryota.
DR   HOGENOM; CLU_260634_0_0_1; -.
DR   InParanoid; Q54S40; -.
DR   OMA; WFNRISS; -.
DR   PRO; PR:Q54S40; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0005085; F:guanyl-nucleotide exchange factor activity; IDA:dictyBase.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0032060; P:bleb assembly; IGI:dictyBase.
DR   GO; GO:0031589; P:cell-substrate adhesion; IMP:dictyBase.
DR   GO; GO:0019934; P:cGMP-mediated signaling; IGI:dictyBase.
DR   GO; GO:0007163; P:establishment or maintenance of cell polarity; IMP:dictyBase.
DR   GO; GO:0031271; P:lateral pseudopodium assembly; IMP:dictyBase.
DR   GO; GO:0043547; P:positive regulation of GTPase activity; IBA:GO_Central.
DR   GO; GO:0007265; P:Ras protein signal transduction; IBA:GO_Central.
DR   GO; GO:0050920; P:regulation of chemotaxis; IMP:dictyBase.
DR   CDD; cd00155; RasGEF; 1.
DR   CDD; cd06224; REM; 1.
DR   Gene3D; 1.10.840.10; -; 1.
DR   Gene3D; 2.30.29.30; -; 1.
DR   Gene3D; 2.60.120.10; -; 2.
DR   InterPro; IPR018490; cNMP-bd-like.
DR   InterPro; IPR000595; cNMP-bd_dom.
DR   InterPro; IPR004182; GRAM.
DR   InterPro; IPR011993; PH-like_dom_sf.
DR   InterPro; IPR008937; Ras-like_GEF.
DR   InterPro; IPR000651; Ras-like_Gua-exchang_fac_N.
DR   InterPro; IPR023578; Ras_GEF_dom_sf.
DR   InterPro; IPR001895; RASGEF_cat_dom.
DR   InterPro; IPR036964; RASGEF_cat_dom_sf.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   PANTHER; PTHR23113; PTHR23113; 1.
DR   Pfam; PF02893; GRAM; 1.
DR   Pfam; PF00617; RasGEF; 1.
DR   Pfam; PF00618; RasGEF_N; 1.
DR   SMART; SM00568; GRAM; 1.
DR   SMART; SM00147; RasGEF; 1.
DR   SMART; SM00229; RasGEFN; 1.
DR   SUPFAM; SSF48366; SSF48366; 1.
DR   SUPFAM; SSF51206; SSF51206; 2.
DR   PROSITE; PS50042; CNMP_BINDING_3; 2.
DR   PROSITE; PS50009; RASGEF_CAT; 1.
DR   PROSITE; PS50212; RASGEF_NTER; 1.
PE   2: Evidence at transcript level;
KW   cGMP; cGMP-binding; Guanine-nucleotide releasing factor;
KW   Nucleotide-binding; Reference proteome; Repeat.
FT   CHAIN           1..1312
FT                   /note="Cyclic GMP-binding protein D"
FT                   /id="PRO_0000353104"
FT   DOMAIN          26..155
FT                   /note="N-terminal Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00135"
FT   DOMAIN          353..582
FT                   /note="Ras-GEF"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00168"
FT   DOMAIN          940..1006
FT                   /note="GRAM"
FT   REGION          206..244
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          260..326
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          586..658
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1059..1108
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          1167..1210
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        206..241
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        586..613
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        1059..1092
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         698..857
FT                   /ligand="a nucleoside 3',5'-cyclic phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58464"
FT                   /ligand_label="1"
FT   BINDING         1105..1218
FT                   /ligand="a nucleoside 3',5'-cyclic phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58464"
FT                   /ligand_label="2"
FT   BINDING         1182..1303
FT                   /ligand="a nucleoside 3',5'-cyclic phosphate"
FT                   /ligand_id="ChEBI:CHEBI:58464"
FT                   /ligand_label="3"
FT   CONFLICT        621
FT                   /note="G -> A (in Ref. 1; AAM34042)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   1312 AA;  143659 MW;  044A4E51DBA2192E CRC64;
     MTDYPFYKPD DETNIIYNIN INKKEGFSAI KSCSLAKLIE KLTSTKQLHT YFSSAFFLTY
     RDFTTPLEII NLLISRYTGP PPDSSKDSLR LFEIEVDIVQ ANVLSIFRTL IGTLIQVDFD
     TPKLSSSIIE FFNSLPESVK NELFLEFFKA KKMARPPGSI PKPVNSNIIS SAASTMRFSY
     SVSPKTQSPN STNNVLSGLL SNNGSNTMNG INGTSNNNVN SNNNNNNGTT NISSPNFDPS
     RSSMKMGKGI MKLLQSNIAN FNNQQNGGGG GGSNNGTSPQ SSPSSTLSST NSLFNQQPSL
     SMLNDDGSVQ NNNNNNNNNN NNNNVNEIST INVQLPPSTP PINGIPGEKT AFLPEAIAKE
     LTIMEWELIT ALSVSDITSK EWNKSVNIQN LTTWFNRISS WVSTKIISKE TPEERAIIIE
     AFINIANFAK ELKNYNCVME ILGSLHGSSI SRLKSSWALL SQKTNDMFQI LNNLMTTDGN
     FKNYRKILTT VLPNEPCIPY LGLFLTDYTY LDESNPSLST DSNLINIDRI FLISTRVQEF
     FQLFTNCNYT FISNMSVRDA ILGEKVWDEN EIFRLSKIRE ETSSLQSLKE SNGIGNSNST
     SGGSSSSLVN KDGSGGGGGS GGGGSVNSKG DGKGDGKDNR DGRGDGNSNC GNGSGISSKD
     KDLLLSSSSS AASHTVRRKN FVTKYRMSFT GNDPLPSVSS TLSEREWKIL TTNSKTIIYP
     RGKTVLSVGE TNTNIYRVIS GRVKVETLKS FNSSDADLGA GGGGGGDNRG EDNSLMAIKA
     RNRLSLNLPA QDDGYYVEEG EIFGQESFLY TDRPMLSNII VDSGECELME IEKSFVLQLF
     ASEHQLSATF YKFIGVIQAQ LLKSIYINFT SNYGNGGSGG SGSNGNGGGS SNGAGGGGLS
     SSVSIGNLNS PNINRIDFKR RSTIFETPSS LDILRDGNDS SFRTKFGLSQ DEVIIKRYQC
     KHNNMNGTLY ITKHNLCFEG KFLGINKNKT IPFDRTDKIL TVDKNIMTVT TKEKVKKFTF
     KSHDDLNEGY GISVQIWANH LNINVKQQLQ LQQQQLQQQQ QQPSQQPSQQ QSQSSQLQQS
     VSASSTTPPV PNSPKVGRGE SFSNIKDLPS KDEWNQILKG TKPLTFKKGE ILICEGVEYQ
     KMFQIVKGEC SVVKSLLPTP LDQNIFNNIN NNNNNNNNNN NNNNNNNNNN NNNNNNGNNN
     NSIFKNNSSI SNTNSNTSID GGVGGGLSPQ TLVDPKNSVI VSKLTHGSIF GEMSFLLPGG
     SATSLVVSSD EVEVYIIESY FLHILLKSKP YLASKFYKYL ACVLETRVRN LT
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024