GBRA4_RAT
ID GBRA4_RAT Reviewed; 552 AA.
AC P28471;
DT 01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-1992, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Gamma-aminobutyric acid receptor subunit alpha-4;
DE AltName: Full=GABA(A) receptor subunit alpha-4;
DE Flags: Precursor;
GN Name=Gabra4;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=1655526; DOI=10.1016/0014-5793(91)81076-k;
RA Wisden W., Herb A., Wieland H., Keinaenen K., Lueddens H., Seeburg P.H.;
RT "Cloning, pharmacological characteristics and expression pattern of the rat
RT GABAA receptor alpha 4 subunit.";
RL FEBS Lett. 289:227-230(1991).
RN [2]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-144, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=24090084; DOI=10.1021/pr400783j;
RA Parker B.L., Thaysen-Andersen M., Solis N., Scott N.E., Larsen M.R.,
RA Graham M.E., Packer N.H., Cordwell S.J.;
RT "Site-specific glycan-peptide analysis for determination of N-glycoproteome
RT heterogeneity.";
RL J. Proteome Res. 12:5791-5800(2013).
CC -!- FUNCTION: GABA, the major inhibitory neurotransmitter in the vertebrate
CC brain, mediates neuronal inhibition by binding to the
CC GABA/benzodiazepine receptor and opening an integral chloride channel.
CC -!- SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor
CC chains: alpha, beta, gamma, delta, and rho.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC protein. Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA4 sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; L08493; AAC42032.1; -; Genomic_DNA.
DR EMBL; S55933; AAB19902.1; -; mRNA.
DR PIR; S17551; S17551.
DR RefSeq; NP_542154.3; NM_080587.3.
DR AlphaFoldDB; P28471; -.
DR SMR; P28471; -.
DR ComplexPortal; CPX-280; GABA-A receptor, alpha-4/beta-3/delta.
DR ComplexPortal; CPX-408; GABA-A receptor, alpha-4/beta-2/delta.
DR CORUM; P28471; -.
DR IntAct; P28471; 2.
DR STRING; 10116.ENSRNOP00000003191; -.
DR BindingDB; P28471; -.
DR ChEMBL; CHEMBL292; -.
DR DrugCentral; P28471; -.
DR GuidetoPHARMACOLOGY; 407; -.
DR GlyGen; P28471; 3 sites, 2 N-linked glycans (1 site).
DR iPTMnet; P28471; -.
DR PhosphoSitePlus; P28471; -.
DR PaxDb; P28471; -.
DR ABCD; P28471; 1 sequenced antibody.
DR Ensembl; ENSRNOT00000003191; ENSRNOP00000003191; ENSRNOG00000002336.
DR GeneID; 140675; -.
DR KEGG; rno:140675; -.
DR UCSC; RGD:621670; rat.
DR CTD; 2557; -.
DR RGD; 621670; Gabra4.
DR eggNOG; KOG3642; Eukaryota.
DR GeneTree; ENSGT00940000159024; -.
DR HOGENOM; CLU_010920_1_6_1; -.
DR InParanoid; P28471; -.
DR OMA; DANLNMR; -.
DR OrthoDB; 1057372at2759; -.
DR Reactome; R-RNO-977443; GABA receptor activation.
DR PRO; PR:P28471; -.
DR Proteomes; UP000002494; Chromosome 14.
DR Bgee; ENSRNOG00000002336; Expressed in frontal cortex and 3 other tissues.
DR Genevisible; P28471; RN.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0032590; C:dendrite membrane; IBA:GO_Central.
DR GO; GO:1902711; C:GABA-A receptor complex; IPI:ComplexPortal.
DR GO; GO:0098982; C:GABA-ergic synapse; IDA:SynGO.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099060; C:integral component of postsynaptic specialization membrane; IDA:SynGO.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0098794; C:postsynapse; IBA:GO_Central.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0004890; F:GABA-A receptor activity; TAS:RGD.
DR GO; GO:0022851; F:GABA-gated chloride ion channel activity; IBA:GO_Central.
DR GO; GO:0005237; F:inhibitory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IDA:SynGO.
DR GO; GO:0007417; P:central nervous system development; ISO:RGD.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0007214; P:gamma-aminobutyric acid signaling pathway; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0060078; P:regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0051932; P:synaptic transmission, GABAergic; IBA:GO_Central.
DR Gene3D; 1.20.58.390; -; 1.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006028; GABAA/Glycine_rcpt.
DR InterPro; IPR001390; GABAAa_rcpt.
DR InterPro; IPR005434; GABBAa4_rcpt.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR01079; GABAARALPHA.
DR PRINTS; PR01617; GABAARALPHA4.
DR PRINTS; PR00253; GABAARECEPTR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Chloride; Chloride channel; Disulfide bond; Glycoprotein;
KW Ion channel; Ion transport; Ligand-gated ion channel; Membrane;
KW Postsynaptic cell membrane; Receptor; Reference proteome; Signal; Synapse;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..35
FT /evidence="ECO:0000255"
FT CHAIN 36..552
FT /note="Gamma-aminobutyric acid receptor subunit alpha-4"
FT /id="PRO_0000000443"
FT TOPO_DOM 36..258
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 259..280
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 285..306
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 318..340
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 341..521
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 522..541
FT /note="Helical"
FT /evidence="ECO:0000305"
FT REGION 353..436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 448..470
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 486..513
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 377..436
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 449..465
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 47
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 144
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0007744|PubMed:24090084"
FT CARBOHYD 157
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 172..186
FT /evidence="ECO:0000250"
SQ SEQUENCE 552 AA; 60951 MW; 3823335C3554115D CRC64;
MVSVQKVPAI VLCSGVSLAL LHVLCLATCL NESPGQNSKD EKLCPENFTR ILDSLLDGYD
NRLRPGFGGP VTEVKTDIYV TSFGPVSDVE MEYTMDVFFR QTWIDKRLKY DGPIEILRLN
NMMVTKVWTP DTFFRNGKKS VSHNMTAPNK LFRIMRNGTI LYTMRLTISA ECPMRLVDFP
MDGHACPLKF GSYAYPKSEM IYTWTKGPEK SVEVPKESSS LVQYDLIGQT VSSETIKSIT
GEYIVMTVYF HLRRKMGYFM IQTYIPCIMT VILSQVSFWI NKESVPARTV FGITTVLTMT
TLSISARHSL PKVSYATAMD WFIAVCFAFV FSALIEFAAV NYFTNIQMQK AKKKISKPPP
EVPAAPVLKE KHTETSLQNT HANLNMRKRT NALVHSESDV NSRTEVGNHS SKTTAAQESS
ETTPKAHLAS SPNPFSRANA AETISAAARG LSSAASPSPH GTLQPAPLRS ASARPAFGAR
LGRIKTTVNT TGVPGNVSAT PPPSAPPPSG SGTSKIDKYA RILFPVTFGA FNMVYWVVYL
SKDTMEKSES LM
