GBRA5_RAT
ID GBRA5_RAT Reviewed; 464 AA.
AC P19969;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 176.
DE RecName: Full=Gamma-aminobutyric acid receptor subunit alpha-5;
DE AltName: Full=GABA(A) receptor subunit alpha-5;
DE Flags: Precursor;
GN Name=Gabra5; Synonyms=Gabra-5;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2561977; DOI=10.1016/0896-6273(89)90243-2;
RA Khrestchatisky M., Maclennan A.J., Chiang M.Y., Xu W., Jackson M.B.,
RA Brecha N., Sternini C., Olsen R.W., Tobin A.J.;
RT "A novel alpha subunit in rat brain GABAA receptors.";
RL Neuron 3:745-753(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=2153588; DOI=10.1016/0014-5793(90)80118-3;
RA Malherbe P., Sigel E., Baur R., Persohn E., Richards J.G., Moehler H.;
RT "Functional expression and sites of gene transcription of a novel alpha
RT subunit of the GABAA receptor in rat brain.";
RL FEBS Lett. 260:261-265(1990).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=2157817; DOI=10.1111/j.1471-4159.1990.tb01237.x;
RA Pritchett D.B., Seeburg P.H.;
RT "Gamma-aminobutyric acidA receptor alpha 5-subunit creates novel type II
RT benzodiazepine receptor pharmacology.";
RL J. Neurochem. 54:1802-1804(1990).
CC -!- FUNCTION: Ligand-gated chloride channel subunit which is a component of
CC the heteropentameric receptor for GABA, the major inhibitory
CC neurotransmitter in the brain. May be involved in GABA-A receptor
CC assembly, and GABA-A receptor immobilization and accumulation by
CC gephyrin at the synapse. {ECO:0000250|UniProtKB:P31644}.
CC -!- SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor
CC chains: alpha, beta, gamma, delta, and rho.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane
CC {ECO:0000250|UniProtKB:P31644}; Multi-pass membrane protein. Cell
CC membrane {ECO:0000250|UniProtKB:P31644}; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRA5 sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; X51992; CAA36248.1; -; mRNA.
DR EMBL; L08494; AAC42033.1; -; Genomic_DNA.
DR PIR; B34130; B34130.
DR RefSeq; NP_058991.1; NM_017295.1.
DR RefSeq; XP_017444562.1; XM_017589073.1.
DR RefSeq; XP_017444563.1; XM_017589074.1.
DR RefSeq; XP_017444564.1; XM_017589075.1.
DR RefSeq; XP_017444565.1; XM_017589076.1.
DR AlphaFoldDB; P19969; -.
DR SMR; P19969; -.
DR BioGRID; 248324; 1.
DR ComplexPortal; CPX-411; GABA-A receptor, alpha-5/beta-3/gamma-2.
DR CORUM; P19969; -.
DR IntAct; P19969; 1.
DR MINT; P19969; -.
DR STRING; 10116.ENSRNOP00000014573; -.
DR BindingDB; P19969; -.
DR ChEMBL; CHEMBL300; -.
DR DrugCentral; P19969; -.
DR GuidetoPHARMACOLOGY; 408; -.
DR CarbonylDB; P19969; -.
DR GlyGen; P19969; 4 sites.
DR iPTMnet; P19969; -.
DR PhosphoSitePlus; P19969; -.
DR PaxDb; P19969; -.
DR PRIDE; P19969; -.
DR ABCD; P19969; 1 sequenced antibody.
DR DNASU; 29707; -.
DR Ensembl; ENSRNOT00000083894; ENSRNOP00000072389; ENSRNOG00000010803.
DR GeneID; 29707; -.
DR KEGG; rno:29707; -.
DR UCSC; RGD:61859; rat.
DR CTD; 2558; -.
DR RGD; 61859; Gabra5.
DR eggNOG; KOG3642; Eukaryota.
DR GeneTree; ENSGT00940000156234; -.
DR InParanoid; P19969; -.
DR OMA; DSKMSNM; -.
DR OrthoDB; 1057372at2759; -.
DR PhylomeDB; P19969; -.
DR TreeFam; TF315453; -.
DR Reactome; R-RNO-977443; GABA receptor activation.
DR PRO; PR:P19969; -.
DR Proteomes; UP000002494; Chromosome 1.
DR Bgee; ENSRNOG00000010803; Expressed in Ammon's horn and 4 other tissues.
DR Genevisible; P19969; RN.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0044297; C:cell body; ISO:RGD.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0005829; C:cytosol; IEA:Ensembl.
DR GO; GO:0030425; C:dendrite; IDA:RGD.
DR GO; GO:0032590; C:dendrite membrane; IBA:GO_Central.
DR GO; GO:1902711; C:GABA-A receptor complex; IBA:GO_Central.
DR GO; GO:0098982; C:GABA-ergic synapse; IDA:SynGO.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099055; C:integral component of postsynaptic membrane; IDA:SynGO.
DR GO; GO:0099060; C:integral component of postsynaptic specialization membrane; IDA:SynGO.
DR GO; GO:0099056; C:integral component of presynaptic membrane; IDA:SynGO.
DR GO; GO:0016020; C:membrane; IDA:RGD.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0032809; C:neuronal cell body membrane; IDA:RGD.
DR GO; GO:0005654; C:nucleoplasm; IEA:Ensembl.
DR GO; GO:0098794; C:postsynapse; IBA:GO_Central.
DR GO; GO:0043235; C:receptor complex; IDA:RGD.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0005254; F:chloride channel activity; TAS:RGD.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0050811; F:GABA receptor binding; IPI:RGD.
DR GO; GO:0004890; F:GABA-A receptor activity; IDA:RGD.
DR GO; GO:0022851; F:GABA-gated chloride ion channel activity; IBA:GO_Central.
DR GO; GO:0005237; F:inhibitory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IDA:SynGO.
DR GO; GO:0008306; P:associative learning; ISO:RGD.
DR GO; GO:0001662; P:behavioral fear response; ISO:RGD.
DR GO; GO:0007420; P:brain development; IEP:RGD.
DR GO; GO:0007268; P:chemical synaptic transmission; IMP:RGD.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0090102; P:cochlea development; ISO:RGD.
DR GO; GO:0007214; P:gamma-aminobutyric acid signaling pathway; IBA:GO_Central.
DR GO; GO:0060119; P:inner ear receptor cell development; ISO:RGD.
DR GO; GO:0060384; P:innervation; ISO:RGD.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0043524; P:negative regulation of neuron apoptotic process; ISO:RGD.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0048666; P:neuron development; ISO:RGD.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0043523; P:regulation of neuron apoptotic process; ISO:RGD.
DR GO; GO:0060078; P:regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0007605; P:sensory perception of sound; ISO:RGD.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0051932; P:synaptic transmission, GABAergic; IBA:GO_Central.
DR Gene3D; 1.20.58.390; -; 1.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006028; GABAA/Glycine_rcpt.
DR InterPro; IPR001390; GABAAa_rcpt.
DR InterPro; IPR005435; GABBAa5_rcpt.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR01079; GABAARALPHA.
DR PRINTS; PR01618; GABAARALPHA5.
DR PRINTS; PR00253; GABAARECEPTR.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Chloride; Chloride channel; Disulfide bond; Glycoprotein;
KW Ion channel; Ion transport; Isopeptide bond; Ligand-gated ion channel;
KW Membrane; Postsynaptic cell membrane; Receptor; Reference proteome; Signal;
KW Synapse; Transmembrane; Transmembrane helix; Transport; Ubl conjugation.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..464
FT /note="Gamma-aminobutyric acid receptor subunit alpha-5"
FT /id="PRO_0000000446"
FT TOPO_DOM 26..259
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 260..281
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..307
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..341
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 342..429
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..451
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 382..414
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 45
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 145
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 207
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 236
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 173..187
FT /evidence="ECO:0000250"
FT CROSSLNK 355
FT /note="Glycyl lysine isopeptide (Lys-Gly) (interchain with
FT G-Cter in ubiquitin)"
FT /evidence="ECO:0000250|UniProtKB:P31644"
FT CONFLICT 126
FT /note="S -> R (in Ref. 2; CAA36248)"
FT /evidence="ECO:0000305"
FT CONFLICT 168
FT /note="T -> M (in Ref. 3; AAC42033)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 464 AA; 52337 MW; 3BC657DDBCBA4298 CRC64;
MDNGMLSRFI MTKTLLVFCI SMTLSSHFGF SQMPTSSVQD ETNDNITIFT RILDGLLDGY
DNRLRPGLGE RITQVRTDIY VTSFGPVSDT EMEYTIDVFF RQSWKDERLR FKGPMQRLPL
NNLLASKIWT PDTFFHNGKK SIAHNMTTPN KLLRLEDDGT LLYTMRLTIS AECPMQLEDF
PMDAHACPLK FGSYAYPNSE VVYVWTNGST KSVVVAEDGS RLNQYHLMGQ TVGTENISTS
TGEYTIMTAH FHLKRKIGYF VIQTYLPCIM TVILSQVSFW LNRESVPART VFGVTTVLTM
TTLSISARNS LPKVAYATAM DWFIAVCYAF VFSALIEFAT VNYFTKRGWA WDGKKALEAA
KIKKKERELI LNKSTNAFTT GKLTHPPNIP KEQLPGGTGN AVGTASIRAS EEKTSESKKT
YNSISKIDKM SRIVFPILFG TFNLVYWATY LNREPVIKGA TSPK
