GBRG3_MOUSE
ID GBRG3_MOUSE Reviewed; 467 AA.
AC P27681; G5E8E8;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 166.
DE RecName: Full=Gamma-aminobutyric acid receptor subunit gamma-3;
DE AltName: Full=GABA(A) receptor subunit gamma-3;
DE Flags: Precursor;
GN Name=Gabrg3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=BALB/C C57; TISSUE=Brain;
RX PubMed=1647983; DOI=10.1016/0014-5793(91)80687-x;
RA Wilson-Shaw D., Robinson M., Alaljev B., Siegel R.E., Sikela J.M.;
RT "A novel gamma subunit of the GABAA receptor identified using the
RT polymerase chain reaction.";
RL FEBS Lett. 284:211-215(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: GABA, the major inhibitory neurotransmitter in the vertebrate
CC brain, mediates neuronal inhibition by binding to the
CC GABA/benzodiazepine receptor and opening an integral chloride channel.
CC -!- SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor
CC chains: alpha, beta, gamma, delta, and rho.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane; Multi-pass membrane
CC protein. Cell membrane; Multi-pass membrane protein.
CC -!- PTM: May be palmitoylated. {ECO:0000250}.
CC -!- MISCELLANEOUS: This subunit carries the benzodiazepine binding site.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRG3 sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; X59300; CAA41988.1; -; mRNA.
DR EMBL; AC102098; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC102129; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC116146; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC134844; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC156610; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC158550; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466599; EDL21863.1; -; Genomic_DNA.
DR CCDS; CCDS21320.1; -.
DR PIR; S16915; S16915.
DR RefSeq; NP_032100.2; NM_008074.2.
DR AlphaFoldDB; P27681; -.
DR SMR; P27681; -.
DR STRING; 10090.ENSMUSP00000067632; -.
DR ChEMBL; CHEMBL2094133; -.
DR DrugCentral; P27681; -.
DR GlyGen; P27681; 2 sites.
DR PhosphoSitePlus; P27681; -.
DR PaxDb; P27681; -.
DR PRIDE; P27681; -.
DR ProteomicsDB; 272941; -.
DR Antibodypedia; 5045; 104 antibodies from 19 providers.
DR DNASU; 14407; -.
DR Ensembl; ENSMUST00000068911; ENSMUSP00000067632; ENSMUSG00000055026.
DR GeneID; 14407; -.
DR KEGG; mmu:14407; -.
DR UCSC; uc009hdz.1; mouse.
DR CTD; 2567; -.
DR MGI; MGI:95624; Gabrg3.
DR VEuPathDB; HostDB:ENSMUSG00000055026; -.
DR eggNOG; KOG3642; Eukaryota.
DR GeneTree; ENSGT00940000155607; -.
DR HOGENOM; CLU_010920_2_0_1; -.
DR InParanoid; P27681; -.
DR OMA; PDSTRWI; -.
DR OrthoDB; 1057372at2759; -.
DR PhylomeDB; P27681; -.
DR TreeFam; TF315453; -.
DR Reactome; R-MMU-977443; GABA receptor activation.
DR BioGRID-ORCS; 14407; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Gabrg3; mouse.
DR PRO; PR:P27681; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; P27681; protein.
DR Bgee; ENSMUSG00000055026; Expressed in epithelium of lens and 68 other tissues.
DR ExpressionAtlas; P27681; baseline and differential.
DR Genevisible; P27681; MM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:0032590; C:dendrite membrane; IBA:GO_Central.
DR GO; GO:1902711; C:GABA-A receptor complex; IBA:GO_Central.
DR GO; GO:0098982; C:GABA-ergic synapse; IDA:SynGO.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0099699; C:integral component of synaptic membrane; IDA:SynGO.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0098794; C:postsynapse; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0005254; F:chloride channel activity; ISO:MGI.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0004890; F:GABA-A receptor activity; ISO:MGI.
DR GO; GO:0022851; F:GABA-gated chloride ion channel activity; IBA:GO_Central.
DR GO; GO:0005237; F:inhibitory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IDA:SynGO.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0007214; P:gamma-aminobutyric acid signaling pathway; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0060078; P:regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0009410; P:response to xenobiotic stimulus; ISO:MGI.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR GO; GO:0051932; P:synaptic transmission, GABAergic; IBA:GO_Central.
DR Gene3D; 1.20.58.390; -; 1.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006028; GABAA/Glycine_rcpt.
DR InterPro; IPR005440; GABBAg3_rcpt.
DR InterPro; IPR005437; GABRG-1/4.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR00253; GABAARECEPTR.
DR PRINTS; PR01620; GABAARGAMMA.
DR PRINTS; PR01623; GABAARGAMMA3.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Chloride; Chloride channel; Disulfide bond; Glycoprotein;
KW Ion channel; Ion transport; Lipoprotein; Membrane; Palmitate;
KW Postsynaptic cell membrane; Reference proteome; Signal; Synapse;
KW Transmembrane; Transmembrane helix; Transport.
FT SIGNAL 1..17
FT /evidence="ECO:0000255"
FT CHAIN 18..467
FT /note="Gamma-aminobutyric acid receptor subunit gamma-3"
FT /id="PRO_0000000482"
FT TOPO_DOM 18..254
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 255..277
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 281..303
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TRANSMEM 315..337
FT /note="Helical"
FT /evidence="ECO:0000305"
FT TOPO_DOM 338..443
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 444..467
FT /note="Helical"
FT /evidence="ECO:0000305"
FT CARBOHYD 110
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 228
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 171..185
FT /evidence="ECO:0000250"
FT CONFLICT 42
FT /note="S -> P (in Ref. 1; CAA41988)"
FT /evidence="ECO:0000305"
FT CONFLICT 47
FT /note="V -> A (in Ref. 1; CAA41988)"
FT /evidence="ECO:0000305"
FT CONFLICT 115
FT /note="I -> T (in Ref. 1; CAA41988)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 467 AA; 54334 MW; E13529EABFB623DD CRC64;
MAAKLLLLLC LFSGLHARSR RVEEDENEDS PSNQKWVLAP KSQDTDVTLI LNKLLREYDK
KLRPDIGIKP TVIDVDIYVN SIGPVSSINM EYQIDIFFAQ TWTDSRLRFN STMKILTLNS
NMVGLIWIPD TIFRNSKTAE AHWITTPNQL LRIWNDGKIL YTLRLTINAE CQLQLHNFPM
DAHACPLTFS SYGYPKEEMI YRWRKNSVEA ADQKSWRLYQ FDFMGLRNTT EIVTTSAGDY
VVMTIYFELS RRMGYFTIQT YIPCILTVVL SWVSFWIKKD ATPARTTLGI TTVLTMTTLS
TIARKSLPRV SYVTAMDLFV TVCFLFVFAA LMEYATLNYY SSCRKPTIRK KKTSLLHPDS
TRWIPDRISL QAPSNYSLLD MRPPPPVMIT LNNSMYWQEF EDTCVYECLD GKDCQSFFCC
YEECKSGSWR RGRIHIDVSE LDSYSRVFFP TSFLLFNLVY WVGYLYL
