GBRP_MOUSE
ID GBRP_MOUSE Reviewed; 440 AA.
AC Q8QZW7; Q3TUT6;
DT 01-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 143.
DE RecName: Full=Gamma-aminobutyric acid receptor subunit pi;
DE AltName: Full=GABA(A) receptor subunit pi;
DE Flags: Precursor;
GN Name=Gabrp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Skin;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Brown J.;
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N, and FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: GABA, the major inhibitory neurotransmitter in the vertebrate
CC brain, mediates neuronal inhibition by binding to the
CC GABA/benzodiazepine receptor and opening an integral chloride channel.
CC In the uterus, the function of the receptor appears to be related to
CC tissue contractility. The binding of this pI subunit with other GABA(A)
CC receptor subunits alters the sensitivity of recombinant receptors to
CC modulatory agents such as pregnanolone (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Generally pentameric. There are five types of GABA(A) receptor
CC chains: alpha, beta, gamma, delta, and epsilon. A sixth class of
CC subunit: Rho form homomeric GABA receptors that do not appear to
CC coexist with GABA(A) receptor subunits but with GABA(C) receptor
CC subunits. Subunit Pi can also bind this complex (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Postsynaptic cell membrane {ECO:0000250}; Multi-
CC pass membrane protein {ECO:0000250}. Cell membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the ligand-gated ion channel (TC 1.A.9) family.
CC Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRP sub-
CC subfamily. {ECO:0000305}.
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DR EMBL; AK028946; BAC26209.1; -; mRNA.
DR EMBL; AK160577; BAE35885.1; -; mRNA.
DR EMBL; AL669814; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC023693; AAH23693.1; -; mRNA.
DR EMBL; BC025550; AAH25550.1; -; mRNA.
DR EMBL; BC027245; AAH27245.1; -; mRNA.
DR EMBL; BC031196; AAH31196.1; -; mRNA.
DR CCDS; CCDS24535.1; -.
DR RefSeq; NP_666129.1; NM_146017.3.
DR AlphaFoldDB; Q8QZW7; -.
DR SMR; Q8QZW7; -.
DR STRING; 10090.ENSMUSP00000020366; -.
DR ChEMBL; CHEMBL2094133; -.
DR DrugCentral; Q8QZW7; -.
DR GlyGen; Q8QZW7; 5 sites.
DR PhosphoSitePlus; Q8QZW7; -.
DR PaxDb; Q8QZW7; -.
DR PRIDE; Q8QZW7; -.
DR ProteomicsDB; 273426; -.
DR Antibodypedia; 4442; 99 antibodies from 20 providers.
DR DNASU; 216643; -.
DR Ensembl; ENSMUST00000020366; ENSMUSP00000020366; ENSMUSG00000020159.
DR GeneID; 216643; -.
DR KEGG; mmu:216643; -.
DR UCSC; uc007ikm.1; mouse.
DR CTD; 2568; -.
DR MGI; MGI:2387597; Gabrp.
DR VEuPathDB; HostDB:ENSMUSG00000020159; -.
DR eggNOG; KOG3643; Eukaryota.
DR GeneTree; ENSGT00940000160813; -.
DR HOGENOM; CLU_010920_0_1_1; -.
DR InParanoid; Q8QZW7; -.
DR OMA; TTVTCNM; -.
DR OrthoDB; 480926at2759; -.
DR PhylomeDB; Q8QZW7; -.
DR TreeFam; TF315453; -.
DR BioGRID-ORCS; 216643; 2 hits in 73 CRISPR screens.
DR ChiTaRS; Gabrp; mouse.
DR PRO; PR:Q8QZW7; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q8QZW7; protein.
DR Bgee; ENSMUSG00000020159; Expressed in trachea and 79 other tissues.
DR ExpressionAtlas; Q8QZW7; baseline and differential.
DR Genevisible; Q8QZW7; MM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0034707; C:chloride channel complex; IEA:UniProtKB-KW.
DR GO; GO:1902711; C:GABA-A receptor complex; IBA:GO_Central.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043005; C:neuron projection; IBA:GO_Central.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0045202; C:synapse; IBA:GO_Central.
DR GO; GO:0005254; F:chloride channel activity; IEA:UniProtKB-KW.
DR GO; GO:0005231; F:excitatory extracellular ligand-gated ion channel activity; IBA:GO_Central.
DR GO; GO:0004890; F:GABA-A receptor activity; ISO:MGI.
DR GO; GO:0030594; F:neurotransmitter receptor activity; IBA:GO_Central.
DR GO; GO:1904315; F:transmitter-gated ion channel activity involved in regulation of postsynaptic membrane potential; IBA:GO_Central.
DR GO; GO:0007268; P:chemical synaptic transmission; IBA:GO_Central.
DR GO; GO:1902476; P:chloride transmembrane transport; IBA:GO_Central.
DR GO; GO:0034220; P:ion transmembrane transport; IBA:GO_Central.
DR GO; GO:0050877; P:nervous system process; IBA:GO_Central.
DR GO; GO:0042391; P:regulation of membrane potential; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR Gene3D; 1.20.58.390; -; 1.
DR Gene3D; 2.70.170.10; -; 1.
DR InterPro; IPR006028; GABAA/Glycine_rcpt.
DR InterPro; IPR008100; GABAAp_rcpt.
DR InterPro; IPR006202; Neur_chan_lig-bd.
DR InterPro; IPR036734; Neur_chan_lig-bd_sf.
DR InterPro; IPR006201; Neur_channel.
DR InterPro; IPR036719; Neuro-gated_channel_TM_sf.
DR InterPro; IPR038050; Neuro_actylchol_rec.
DR InterPro; IPR006029; Neurotrans-gated_channel_TM.
DR InterPro; IPR018000; Neurotransmitter_ion_chnl_CS.
DR PANTHER; PTHR18945; PTHR18945; 1.
DR PANTHER; PTHR18945:SF33; PTHR18945:SF33; 1.
DR Pfam; PF02931; Neur_chan_LBD; 1.
DR Pfam; PF02932; Neur_chan_memb; 1.
DR PRINTS; PR00253; GABAARECEPTR.
DR PRINTS; PR01724; GABAARPI.
DR PRINTS; PR00252; NRIONCHANNEL.
DR SUPFAM; SSF63712; SSF63712; 1.
DR SUPFAM; SSF90112; SSF90112; 1.
DR TIGRFAMs; TIGR00860; LIC; 1.
DR PROSITE; PS00236; NEUROTR_ION_CHANNEL; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Chloride; Chloride channel; Disulfide bond; Glycoprotein;
KW Ion channel; Ion transport; Membrane; Postsynaptic cell membrane;
KW Reference proteome; Signal; Synapse; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..21
FT /evidence="ECO:0000255"
FT CHAIN 22..440
FT /note="Gamma-aminobutyric acid receptor subunit pi"
FT /id="PRO_0000000493"
FT TOPO_DOM 22..242
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 305..327
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 328..416
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 417..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CARBOHYD 43
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 102
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 145
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 196
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 228
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 160..174
FT /evidence="ECO:0000250"
SQ SEQUENCE 440 AA; 50436 MW; DF8D2AAB3300D445 CRC64;
MSYSLYLAFL CLSLLTQRTC IQGNQVNVEV SRSDKLSLPG FENLTAGYNK FLRPNFGGDP
VRIALTLDIA SISSISESNM DYTATIYLRQ RWTDPRLVFE GNKSFTLDAR LVEFLWVPDT
YIVESKKSFL HEVTVGNRLI RLFSNGTVLY ALRITTTVTC NMDLSKYPMD TQTCKLQLES
WGYDGNDVEF SWLRGNDSVR GLENLRLAQY TIQQYFTLVT VSQQETGNYT RLVLQFELRR
NVLYFILETY VPSTFLVVLS WVSFWISLDS VPARTCIGVT TVLSMTTLMI GSRTSLPNTN
CFIKAIDVYL GICFSFVFGA LLEYAVAHYS SLQQMAVKDR GPAKDSEEVN ITNIINSSIS
SFKRKISFAS IEISGDNVNY SDLTMKASDK FKFVFREKIS RIIDYFTIQN PSNVDRYSKL
LFPLIFMLAN VFYWAYYMYF
