GCD1_ARATH
ID GCD1_ARATH Reviewed; 420 AA.
AC Q9LVA9; F4K6F8;
DT 17-JUN-2020, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Protein GAMETE CELL DEFECTIVE 1, mitochondrial {ECO:0000303|PubMed:23085019};
DE Short=AtGCD1 {ECO:0000303|PubMed:23085019};
DE Flags: Precursor;
GN Name=GCD1 {ECO:0000303|PubMed:23085019};
GN OrderedLocusNames=At5g62270 {ECO:0000312|Araport:AT5G62270};
GN ORFNames=MMI9.10 {ECO:0000312|EMBL:BAA97191.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10718197; DOI=10.1093/dnares/7.1.31;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence
RT features of the regions of 3,076,755 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:31-63(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP FUNCTION, DISRUPTION PHENOTYPE, SUBCELLULAR LOCATION, TISSUE SPECIFICITY,
RP AND DEVELOPMENTAL STAGE.
RX PubMed=23085019; DOI=10.1016/j.devcel.2012.09.011;
RA Wu J.-J., Peng X.-B., Li W.-W., He R., Xin H.-P., Sun M.-X.;
RT "Mitochondrial GCD1 dysfunction reveals reciprocal cell-to-cell signaling
RT during the maturation of Arabidopsis female gametes.";
RL Dev. Cell 23:1043-1058(2012).
CC -!- FUNCTION: Essential for fertility (male and female gametophyte
CC functions and development) (PubMed:23085019). Required for the
CC integrity of female gametic mitochondria (PubMed:23085019). Modulates
CC male gametophyte functions, including pollen tube growth and style
CC penetration (PubMed:23085019). Involved in mitochondrial-driven cell-
CC to-cell communication in embryo sacs during female gametes maturation
CC (including embryogenesis initiation and endosperm development),
CC especially for reciprocal signaling between central and egg cells which
CC regulates reciprocal development (PubMed:23085019).
CC {ECO:0000269|PubMed:23085019}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000269|PubMed:23085019}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9LVA9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9LVA9-2; Sequence=VSP_060591, VSP_060592;
CC -!- TISSUE SPECIFICITY: Expressed at high levels in reproductive organs
CC and, at lower levels, ubiquitously. {ECO:0000269|PubMed:23085019}.
CC -!- DEVELOPMENTAL STAGE: In flowers, accumulates in the female gametophytes
CC at different stages, as well as in early embryos and endosperms.
CC {ECO:0000269|PubMed:23085019}.
CC -!- DISRUPTION PHENOTYPE: Irregular structure and reduced number of cristae
CC in female gametic mitochondria (PubMed:23085019). Completely disrupted
CC male gametophyte functioning (shortened pollen tubes leading to altered
CC style penetration) and impaired female gametes final maturation of the
CC egg and central cells (persistent antipodal cells in embryo sacs, as
CC well as small and short egg cells), not required for double
CC fertilization, but essential for embryogenesis initiation and endosperm
CC development, thus leading to the presence of aborted seeds in siliques
CC (PubMed:23085019). {ECO:0000269|PubMed:23085019}.
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DR EMBL; AB019235; BAA97191.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97588.1; -; Genomic_DNA.
DR EMBL; CP002688; AED97589.1; -; Genomic_DNA.
DR RefSeq; NP_001190596.1; NM_001203667.2. [Q9LVA9-1]
DR RefSeq; NP_201033.2; NM_125621.4. [Q9LVA9-2]
DR PDB; 6XYW; EM; 3.86 A; BD=1-420.
DR PDBsum; 6XYW; -.
DR AlphaFoldDB; Q9LVA9; -.
DR SMR; Q9LVA9; -.
DR IntAct; Q9LVA9; 3.
DR STRING; 3702.AT5G62270.2; -.
DR PaxDb; Q9LVA9; -.
DR PRIDE; Q9LVA9; -.
DR ProteomicsDB; 220120; -.
DR EnsemblPlants; AT5G62270.1; AT5G62270.1; AT5G62270. [Q9LVA9-2]
DR EnsemblPlants; AT5G62270.2; AT5G62270.2; AT5G62270. [Q9LVA9-1]
DR GeneID; 836348; -.
DR Gramene; AT5G62270.1; AT5G62270.1; AT5G62270. [Q9LVA9-2]
DR Gramene; AT5G62270.2; AT5G62270.2; AT5G62270. [Q9LVA9-1]
DR KEGG; ath:AT5G62270; -.
DR Araport; AT5G62270; -.
DR TAIR; locus:2167998; AT5G62270.
DR eggNOG; ENOG502QPTE; Eukaryota.
DR HOGENOM; CLU_058322_0_0_1; -.
DR InParanoid; Q9LVA9; -.
DR OMA; ANGWKER; -.
DR OrthoDB; 906019at2759; -.
DR PhylomeDB; Q9LVA9; -.
DR PRO; PR:Q9LVA9; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9LVA9; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0007154; P:cell communication; IMP:UniProtKB.
DR GO; GO:0009793; P:embryo development ending in seed dormancy; IMP:UniProtKB.
DR GO; GO:0010342; P:endosperm cellularization; IEA:EnsemblPlants.
DR GO; GO:0009960; P:endosperm development; IEA:EnsemblPlants.
DR GO; GO:0048229; P:gametophyte development; IMP:TAIR.
DR GO; GO:0007006; P:mitochondrial membrane organization; IMP:TAIR.
DR GO; GO:0051647; P:nucleus localization; IEA:EnsemblPlants.
DR GO; GO:0009555; P:pollen development; IEA:EnsemblPlants.
DR GO; GO:0009846; P:pollen germination; IEA:EnsemblPlants.
DR GO; GO:0048868; P:pollen tube development; IMP:UniProtKB.
DR GO; GO:0010468; P:regulation of gene expression; IEA:EnsemblPlants.
DR GO; GO:0043067; P:regulation of programmed cell death; IEA:EnsemblPlants.
DR GO; GO:0010581; P:regulation of starch biosynthetic process; IEA:EnsemblPlants.
DR GO; GO:0007033; P:vacuole organization; IEA:EnsemblPlants.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Developmental protein; Mitochondrion;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..11
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 12..420
FT /note="Protein GAMETE CELL DEFECTIVE 1, mitochondrial"
FT /id="PRO_0000450244"
FT REGION 99..120
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 390..420
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 100..117
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 395..412
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 382..383
FT /note="SI -> KI (in isoform 2)"
FT /id="VSP_060591"
FT VAR_SEQ 384..420
FT /note="Missing (in isoform 2)"
FT /id="VSP_060592"
SQ SEQUENCE 420 AA; 48314 MW; D9CC5F521637EDD4 CRC64;
MYNLSRIIYR FSSVSLNPST RASGFLLENA SSKILQSATN RAFSAKSGSD GVGGDDNGWN
ISTGGSFGGT GSADLDWDNK SMWSTGLTKE HFDGVSVGRQ KNAANPSSDN TPSDSGDVMS
KLGPKEVALV NEMNEYDDLL KEIEQDNRQG RAFVDGIKQR MMEISVLLKQ VKEPGARGSY
LKDSEKTEMY RLHKENPEVY TIERLAKDYR IMRQRVHAIL FLKEDEEEEE RKLGRPLDDS
VDRLLDEYPE FFISHDREFH VASLNYKPDF KVMPEGWDGT IKDMDEVHYE ISKKEDDMLY
EEFVRRFEFN KMKWRGEVMC HKYSRRRSSE GWKITVEKLG AKGKRGAGGG WKFMSLPDGS
SRPLNEMEKV YVKRETPLRR RSIISTEVMR YSPANQKRRS KRKQKRKERR IACLKAGKQT
