GCDC_ACIFV
ID GCDC_ACIFV Reviewed; 145 AA.
AC Q9ZAA7; D2RM87;
DT 16-JAN-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1999, sequence version 1.
DT 03-AUG-2022, entry version 110.
DE RecName: Full=Glutaconyl-CoA decarboxylase subunit gamma;
DE EC=7.2.4.5;
DE AltName: Full=Biotin carrier;
GN Name=gcdC; OrderedLocusNames=Acfer_1835;
OS Acidaminococcus fermentans (strain ATCC 25085 / DSM 20731 / CCUG 9996 / CIP
OS 106432 / VR4).
OC Bacteria; Firmicutes; Negativicutes; Acidaminococcales; Acidaminococcaceae;
OC Acidaminococcus.
OX NCBI_TaxID=591001;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-34.
RX PubMed=10027965; DOI=10.1046/j.1365-2958.1999.01189.x;
RA Braune A., Bendrat K., Rospert S., Buckel W.;
RT "The sodium ion translocating glutaconyl-CoA decarboxylase from
RT Acidaminococcus fermentans: cloning and function of the genes forming a
RT second operon.";
RL Mol. Microbiol. 31:473-487(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25085 / DSM 20731 / CCUG 9996 / CIP 106432 / VR4;
RX PubMed=21304687; DOI=10.4056/sigs.1002553;
RA Chang Y.J., Pukall R., Saunders E., Lapidus A., Copeland A., Nolan M.,
RA Glavina Del Rio T., Lucas S., Chen F., Tice H., Cheng J.F., Han C.,
RA Detter J.C., Bruce D., Goodwin L., Pitluck S., Mikhailova N., Liolios K.,
RA Pati A., Ivanova N., Mavromatis K., Chen A., Palaniappan K., Land M.,
RA Hauser L., Jeffries C.D., Brettin T., Rohde M., Goker M., Bristow J.,
RA Eisen J.A., Markowitz V., Hugenholtz P., Kyrpides N.C., Klenk H.P.;
RT "Complete genome sequence of Acidaminococcus fermentans type strain
RT (VR4).";
RL Stand. Genomic Sci. 3:1-14(2010).
CC -!- FUNCTION: Biotin carrier subunit of the primary sodium pump glutaconyl-
CC CoA decarboxylase (GCD).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2E)-glutaconyl-CoA + H(+) + Na(+)(in) = (2E)-butenoyl-CoA +
CC CO2 + Na(+)(out); Xref=Rhea:RHEA:23972, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:29101, ChEBI:CHEBI:57332,
CC ChEBI:CHEBI:57353; EC=7.2.4.5;
CC -!- COFACTOR:
CC Name=biotin; Xref=ChEBI:CHEBI:57586;
CC -!- PATHWAY: Amino-acid degradation; L-glutamate degradation via
CC hydroxyglutarate pathway; crotonoyl-CoA from L-glutamate: step 5/5.
CC -!- SUBUNIT: Heterooctamer consisting of two alpha, two beta, two gamma and
CC two delta subunits.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF030576; AAC69172.1; -; Genomic_DNA.
DR EMBL; CP001859; ADB48189.1; -; Genomic_DNA.
DR RefSeq; WP_012939172.1; NC_013740.1.
DR AlphaFoldDB; Q9ZAA7; -.
DR SMR; Q9ZAA7; -.
DR STRING; 591001.Acfer_1835; -.
DR TCDB; 3.B.1.1.3; the na(+)-transporting carboxylic acid decarboxylase (nat-dc) family.
DR EnsemblBacteria; ADB48189; ADB48189; Acfer_1835.
DR KEGG; afn:Acfer_1835; -.
DR eggNOG; COG4770; Bacteria.
DR HOGENOM; CLU_016733_5_2_9; -.
DR OMA; AIHVGPG; -.
DR OrthoDB; 1938042at2; -.
DR BioCyc; MetaCyc:MON-1056; -.
DR BRENDA; 7.2.4.5; 85.
DR SABIO-RK; Q9ZAA7; -.
DR UniPathway; UPA00533; UER00688.
DR Proteomes; UP000001902; Chromosome.
DR GO; GO:0018801; F:glutaconyl-CoA decarboxylase activity; IEA:UniProtKB-EC.
DR GO; GO:0019552; P:glutamate catabolic process via 2-hydroxyglutarate; IEA:UniProtKB-UniPathway.
DR GO; GO:0006814; P:sodium ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR001882; Biotin_BS.
DR InterPro; IPR000089; Biotin_lipoyl.
DR InterPro; IPR011053; Single_hybrid_motif.
DR Pfam; PF00364; Biotin_lipoyl; 1.
DR SUPFAM; SSF51230; SSF51230; 1.
DR PROSITE; PS00188; BIOTIN; 1.
DR PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
PE 1: Evidence at protein level;
KW Biotin; Decarboxylase; Direct protein sequencing; Ion transport; Lyase;
KW Reference proteome; Sodium; Sodium transport; Translocase; Transport.
FT CHAIN 1..145
FT /note="Glutaconyl-CoA decarboxylase subunit gamma"
FT /id="PRO_0000146839"
FT DOMAIN 77..145
FT /note="Biotinyl-binding"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01066"
FT REGION 52..82
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 112
FT /note="N6-biotinyllysine"
FT /evidence="ECO:0000250, ECO:0000255|PROSITE-
FT ProRule:PRU01066"
SQ SEQUENCE 145 AA; 13908 MW; 4546006D4F2F4C6B CRC64;
MRKFNVNVNG TVYTVEVEEV GGAVTAAPAA PAAPAAAPAA APVAAAPAAA PAPAPAAAPA
AAPAPAAKPA AAAPAGSVTV SAPMPGKILS VNVKPGDKVE AGDVLLILEA MKMQNEIMAP
EDGTVSEVRV NAGDTVATGD VMVIL
