GCH1L_METJA
ID GCH1L_METJA Reviewed; 244 AA.
AC Q58337;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 15-MAY-2002, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=GTP cyclohydrolase 1 type 2 homolog;
GN OrderedLocusNames=MJ0927;
OS Methanocaldococcus jannaschii (strain ATCC 43067 / DSM 2661 / JAL-1 / JCM
OS 10045 / NBRC 100440) (Methanococcus jannaschii).
OC Archaea; Euryarchaeota; Methanomada group; Methanococci; Methanococcales;
OC Methanocaldococcaceae; Methanocaldococcus.
OX NCBI_TaxID=243232;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 43067 / DSM 2661 / JAL-1 / JCM 10045 / NBRC 100440;
RX PubMed=8688087; DOI=10.1126/science.273.5278.1058;
RA Bult C.J., White O., Olsen G.J., Zhou L., Fleischmann R.D., Sutton G.G.,
RA Blake J.A., FitzGerald L.M., Clayton R.A., Gocayne J.D., Kerlavage A.R.,
RA Dougherty B.A., Tomb J.-F., Adams M.D., Reich C.I., Overbeek R.,
RA Kirkness E.F., Weinstock K.G., Merrick J.M., Glodek A., Scott J.L.,
RA Geoghagen N.S.M., Weidman J.F., Fuhrmann J.L., Nguyen D., Utterback T.R.,
RA Kelley J.M., Peterson J.D., Sadow P.W., Hanna M.C., Cotton M.D.,
RA Roberts K.M., Hurst M.A., Kaine B.P., Borodovsky M., Klenk H.-P.,
RA Fraser C.M., Smith H.O., Woese C.R., Venter J.C.;
RT "Complete genome sequence of the methanogenic archaeon, Methanococcus
RT jannaschii.";
RL Science 273:1058-1073(1996).
RN [2]
RP CRYSTALLIZATION.
RX PubMed=23295494; DOI=10.1107/s1744309112049408;
RA Kuan S.M., Chen H.C., Huang C.H., Chang C.H., Chen S.C., Yang C.S.,
RA Chen Y.;
RT "Crystallization and preliminary X-ray diffraction analysis of the Nif3-
RT family protein MJ0927 from Methanocaldococcus jannaschii.";
RL Acta Crystallogr. F 69:80-82(2013).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.47 ANGSTROMS), AND DNA-BINDING.
RX PubMed=25243119; DOI=10.1155/2014/171263;
RA Chen S.C., Huang C.H., Yang C.S., Kuan S.M., Lin C.T., Chou S.H., Chen Y.;
RT "Crystal structure of a conserved hypothetical protein MJ0927 from
RT Methanocaldococcus jannaschii reveals a novel quaternary assembly in the
RT Nif3 family.";
RL Biomed. Res. Int. 2014:171263-171263(2014).
CC -!- FUNCTION: DNA-binding protein exhibiting the ability to bind to both
CC single-stranded and double-stranded DNA. {ECO:0000269|PubMed:25243119}.
CC -!- SUBUNIT: Homohexamer; trimer of dimers, that forms a hollow cage-like
CC architecture. {ECO:0000269|PubMed:25243119}.
CC -!- SIMILARITY: Belongs to the GTP cyclohydrolase I type 2/NIF3 family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB98929.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; L77117; AAB98929.1; ALT_INIT; Genomic_DNA.
DR PIR; G64415; G64415.
DR RefSeq; WP_064496667.1; NC_000909.1.
DR PDB; 4IWG; X-ray; 2.47 A; A/B/C=1-244.
DR PDB; 4IWM; X-ray; 2.70 A; A/B/C/D/E/F=1-244.
DR PDBsum; 4IWG; -.
DR PDBsum; 4IWM; -.
DR AlphaFoldDB; Q58337; -.
DR SMR; Q58337; -.
DR MINT; Q58337; -.
DR STRING; 243232.MJ_0927; -.
DR EnsemblBacteria; AAB98929; AAB98929; MJ_0927.
DR GeneID; 1451816; -.
DR KEGG; mja:MJ_0927; -.
DR eggNOG; arCOG04454; Archaea.
DR HOGENOM; CLU_037423_2_0_2; -.
DR InParanoid; Q58337; -.
DR OMA; THHSKIL; -.
DR OrthoDB; 40682at2157; -.
DR PhylomeDB; Q58337; -.
DR Proteomes; UP000000805; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR InterPro; IPR002678; DUF34/NIF3.
DR InterPro; IPR036069; DUF34/NIF3_sf.
DR PANTHER; PTHR13799; PTHR13799; 1.
DR Pfam; PF01784; NIF3; 1.
DR SUPFAM; SSF102705; SSF102705; 1.
DR TIGRFAMs; TIGR00486; YbgI_SA1388; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Metal-binding; Reference proteome.
FT CHAIN 1..244
FT /note="GTP cyclohydrolase 1 type 2 homolog"
FT /id="PRO_0000147348"
FT BINDING 65
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P0AFP6"
FT BINDING 66
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P0AFP6"
FT BINDING 102
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P0AFP6"
FT BINDING 216
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P0AFP6"
FT BINDING 220
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="1"
FT /evidence="ECO:0000250|UniProtKB:P0AFP6"
FT BINDING 220
FT /ligand="a divalent metal cation"
FT /ligand_id="ChEBI:CHEBI:60240"
FT /ligand_label="2"
FT /evidence="ECO:0000250|UniProtKB:P0AFP6"
FT HELIX 3..13
FT /evidence="ECO:0007829|PDB:4IWG"
FT HELIX 16..18
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 26..29
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 39..44
FT /evidence="ECO:0007829|PDB:4IWG"
FT HELIX 48..56
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 60..66
FT /evidence="ECO:0007829|PDB:4IWG"
FT HELIX 78..89
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 93..96
FT /evidence="ECO:0007829|PDB:4IWG"
FT HELIX 99..103
FT /evidence="ECO:0007829|PDB:4IWG"
FT HELIX 108..115
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 119..125
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 131..134
FT /evidence="ECO:0007829|PDB:4IWG"
FT HELIX 139..149
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 155..157
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 167..174
FT /evidence="ECO:0007829|PDB:4IWG"
FT HELIX 178..184
FT /evidence="ECO:0007829|PDB:4IWG"
FT TURN 185..187
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 189..194
FT /evidence="ECO:0007829|PDB:4IWG"
FT HELIX 198..207
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 210..213
FT /evidence="ECO:0007829|PDB:4IWG"
FT HELIX 216..234
FT /evidence="ECO:0007829|PDB:4IWG"
FT STRAND 237..242
FT /evidence="ECO:0007829|PDB:4IWG"
SQ SEQUENCE 244 AA; 27363 MW; BC42E5E29AAE399F CRC64;
MKAKEIIEFI ETFAPKDLAI EGDNIGLQVG DNLDKEIKKL GIALDPSLSV IKKAEKEGVD
FLFTHHPLLK DPIRNFTGVI YKKLKILMEN DIILYSAHTN LDICKNGLND ALAELYNLEN
PKPLYDNGLG RVGIFKGSFE EFLEITKKYI HKNPIVVKSK EVDDNFKLAV LSGYGLSQSS
IKYVAEKADV YLSGDLTHHS KILAEELGLV VVDATHYSTE VFGLKKFKEF LSSNLDLEII
SLDF
