ID   3NOJ_BUNCA              Reviewed;          63 AA.
AC   P81782;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   30-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Bucandin {ECO:0000303|PubMed:11053837};
OS   Bungarus candidus (Malayan krait).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Bungarinae; Bungarus.
OX   NCBI_TaxID=92438;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, X-RAY CRYSTALLOGRAPHY (0.97 ANGSTROMS),
RP   DISULFIDE BONDS, SUBCELLULAR LOCATION, AND MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=11053837; DOI=10.1107/s0907444900011501;
RA   Kuhn P., Deacon A.M., Comsa D.S., Rajaseger G., Kini R.M., Uson I.I.,
RA   Kolatkar P.R.;
RT   "The atomic resolution structure of bucandin, a novel toxin isolated from
RT   the Malayan krait, determined by direct methods.";
RL   Acta Crystallogr. D 56:1401-1407(2000).
RN   [2]
RP   ERRATUM OF PUBMED:11053837.
RX   PubMed=11092952; DOI=10.1107/s090744490001739x;
RA   Kuhn P., Deacon A.M., Comsa D.S., Rajaseger G., Kini R.M., Uson I.I.,
RA   Kolatkar P.R.;
RL   Acta Crystallogr. D 56:1702-1702(2000).
RN   [3]
RP   STRUCTURE BY NMR, AND DISULFIDE BONDS.
RC   TISSUE=Venom;
RX   PubMed=11736642; DOI=10.1042/0264-6021:3600539;
RA   Torres A.M., Kini R.M., Selvanayagam N., Kuchel P.W.;
RT   "NMR structure of bucandin, a neurotoxin from the venom of the Malayan
RT   krait (Bungarus candidus).";
RL   Biochem. J. 360:539-548(2001).
CC   -!- FUNCTION: This toxin is described as enhancing presynaptic
CC       acetylcholine release, but neither experimental results, nor references
CC       to other sources are available. {ECO:0000303|PubMed:11053837}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11053837}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland. {ECO:0000305}.
CC   -!- MASS SPECTROMETRY: Mass=7275.43; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:11053837};
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Ancestral
CC       subfamily. Orphan group XIX sub-subfamily. {ECO:0000305}.
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DR   PDB; 1F94; X-ray; 0.97 A; A=1-63.
DR   PDB; 1IJC; NMR; -; A=1-63.
DR   PDBsum; 1F94; -.
DR   PDBsum; 1IJC; -.
DR   AlphaFoldDB; P81782; -.
DR   SMR; P81782; -.
DR   TCDB; 8.B.23.2.3; the mambalgin (mambalgin) family.
DR   EvolutionaryTrace; P81782; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing; Disulfide bond; Neurotoxin;
KW   Presynaptic neurotoxin; Secreted; Toxin.
FT   CHAIN           1..63
FT                   /note="Bucandin"
FT                   /evidence="ECO:0000269|PubMed:11053837"
FT                   /id="PRO_0000093534"
FT   DISULFID        3..24
FT                   /evidence="ECO:0000269|PubMed:11053837,
FT                   ECO:0000312|PDB:1F94, ECO:0000312|PDB:1IJC"
FT   DISULFID        6..11
FT                   /evidence="ECO:0000269|PubMed:11053837,
FT                   ECO:0000312|PDB:1F94, ECO:0000312|PDB:1IJC"
FT   DISULFID        17..39
FT                   /evidence="ECO:0000269|PubMed:11053837,
FT                   ECO:0000312|PDB:1F94, ECO:0000312|PDB:1IJC"
FT   DISULFID        43..55
FT                   /evidence="ECO:0000269|PubMed:11053837,
FT                   ECO:0000312|PDB:1F94, ECO:0000312|PDB:1IJC"
FT   DISULFID        56..61
FT                   /evidence="ECO:0000269|PubMed:11053837,
FT                   ECO:0000312|PDB:1F94, ECO:0000312|PDB:1IJC"
FT   STRAND          2..7
FT                   /evidence="ECO:0007829|PDB:1F94"
FT   STRAND          10..16
FT                   /evidence="ECO:0007829|PDB:1F94"
FT   STRAND          23..29
FT                   /evidence="ECO:0007829|PDB:1F94"
FT   TURN            30..32
FT                   /evidence="ECO:0007829|PDB:1F94"
FT   STRAND          35..41
FT                   /evidence="ECO:0007829|PDB:1F94"
FT   STRAND          46..56
FT                   /evidence="ECO:0007829|PDB:1F94"
SQ   SEQUENCE   63 AA;  7285 MW;  D16EB0A0BCBA7AC0 CRC64;
     MECYRCGVSG CHLKITCSAE ETFCYKWLNK ISNERWLGCA KTCTEIDTWN VYNKCCTTNL
     CNT