ID   3NOJ_DENAN              Reviewed;          63 AA.
AC   C0HJT4;
DT   08-JUN-2016, integrated into UniProtKB/Swiss-Prot.
DT   08-JUN-2016, sequence version 1.
DT   25-MAY-2022, entry version 14.
DE   RecName: Full=Toxin Tx7335 {ECO:0000303|PubMed:27044983};
OS   Dendroaspis angusticeps (Eastern green mamba) (Naja angusticeps).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Dendroaspis.
OX   NCBI_TaxID=8618;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY.
RC   TISSUE=Venom;
RX   PubMed=27044983; DOI=10.1038/srep23904;
RA   Rivera-Torres I.O., Jin T.B., Cadene M., Chait B.T., Poget S.F.;
RT   "Discovery and characterisation of a novel toxin from Dendroaspis
RT   angusticeps, named Tx7335, that activates the potassium channel KcsA.";
RL   Sci. Rep. 6:23904-23904(2016).
CC   -!- FUNCTION: Activates bacterial pH-gated potassium channel KcsA by
CC       binding to its extracellular domain, probably at a site different from
CC       channel inhibitors. Increases both mean open time and open probability
CC       of KscA. {ECO:0000269|PubMed:27044983}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:27044983}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:27044983}.
CC   -!- PTM: Contains 4 disulfide bonds. {ECO:0000269|PubMed:27044983}.
CC   -!- MASS SPECTROMETRY: Mass=7333.5; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:27044983};
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Ancestral
CC       subfamily. Orphan group XIX sub-subfamily. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   AlphaFoldDB; C0HJT4; -.
DR   SMR; C0HJT4; -.
DR   TCDB; 8.B.23.2.1; the mambalgin (mambalgin) family.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Secreted; Toxin.
FT   PEPTIDE         1..63
FT                   /note="Toxin Tx7335"
FT                   /evidence="ECO:0000269|PubMed:27044983"
FT                   /id="PRO_0000436390"
FT   DISULFID        3..24
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        17..39
FT                   /evidence="ECO:0000269|PubMed:27044983"
FT   DISULFID        25..55
FT                   /evidence="ECO:0000305|PubMed:27044983"
FT   DISULFID        56..61
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
SQ   SEQUENCE   63 AA;  7344 MW;  AD32A630E69F4FF6 CRC64;
     LECHRRGSFI SDGKITCSAK KTFCCKMYEK IFGIYWYGCA KTYTEKNTWN VYSKCCTTNL
     CNT