ID   3NOJ_WALAE              Reviewed;          63 AA.
AC   C0HKZ8;
DT   27-SEP-2017, integrated into UniProtKB/Swiss-Prot.
DT   18-JUL-2018, sequence version 2.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=Actiflagelin {ECO:0000303|PubMed:29410678};
OS   Walterinnesia aegyptia (Desert black snake).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Lepidosauria; Squamata; Bifurcata; Unidentata; Episquamata; Toxicofera;
OC   Serpentes; Colubroidea; Elapidae; Elapinae; Walterinnesia.
OX   NCBI_TaxID=64182;
RN   [1]
RP   PROTEIN SEQUENCE, FUNCTION, SUBCELLULAR LOCATION, MASS SPECTROMETRY,
RP   PRESENCE OF DISULFIDE BONDS, AND PROBABLE AMIDATION AT PRO-63.
RC   TISSUE=Venom;
RX   PubMed=29410678; DOI=10.1186/s40409-018-0140-4;
RA   Abd El-Aziz T.M., Al Khoury S., Jaquillard L., Triquigneaux M.,
RA   Martinez G., Bourgoin-Voillard S., Seve M., Arnoult C., Beroud R.,
RA   De Waard M.;
RT   "Actiflagelin, a new sperm activator isolated from Walterinnesia aegyptia
RT   venom using phenotypic screening.";
RL   J. Venom. Anim. Toxins Incl. Trop. Dis. 24:2-2(2018).
CC   -!- FUNCTION: Unknown. In vitro, this toxin has been shown to activate
CC       sperm motility when tested on OF1 male mice.
CC       {ECO:0000269|PubMed:29410678}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29410678}.
CC   -!- TISSUE SPECIFICITY: Expressed by the venom gland.
CC       {ECO:0000305|PubMed:29410678}.
CC   -!- PTM: Contains 5 disulfide bonds. {ECO:0000269|PubMed:29410678}.
CC   -!- MASS SPECTROMETRY: Mass=7329.8; Method=MALDI;
CC       Evidence={ECO:0000269|PubMed:29410678};
CC   -!- MASS SPECTROMETRY: Mass=7329.71; Mass_error=0.05; Method=Electrospray;
CC       Evidence={ECO:0000269|PubMed:29410678};
CC   -!- SIMILARITY: Belongs to the snake three-finger toxin family. Ancestral
CC       subfamily. Orphan group XIX sub-subfamily. {ECO:0000305}.
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DR   AlphaFoldDB; C0HKZ8; -.
DR   SMR; C0HKZ8; -.
DR   GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR   GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.10.60.10; -; 1.
DR   InterPro; IPR045860; Snake_toxin-like_sf.
DR   SUPFAM; SSF57302; SSF57302; 1.
PE   1: Evidence at protein level;
KW   Amidation; Direct protein sequencing; Disulfide bond; Neurotoxin;
KW   Presynaptic neurotoxin; Secreted; Toxin.
FT   CHAIN           1..63
FT                   /note="Actiflagelin"
FT                   /evidence="ECO:0000269|PubMed:29410678"
FT                   /id="PRO_0000441734"
FT   MOD_RES         63
FT                   /note="Proline amide"
FT                   /evidence="ECO:0000305|PubMed:29410678"
FT   DISULFID        3..24
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        6..11
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        17..39
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        43..55
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
FT   DISULFID        56..61
FT                   /evidence="ECO:0000250|UniProtKB:P81782"
SQ   SEQUENCE   63 AA;  7340 MW;  DACD2147DC792FC0 CRC64;
     MECYKCGVSG CHLKITCSAE EKFCYKWRDK ISNERWLGCA KTCTEENTWR VYNSCCTTNL
     CNP