GCL_DROME
ID GCL_DROME Reviewed; 569 AA.
AC Q01820; Q9V4X5;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=Protein germ cell-less;
GN Name=gcl; ORFNames=CG8411;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=1380406; DOI=10.1016/0092-8674(92)90427-e;
RA Jongens T.A., Hay B., Jan L.Y., Jan Y.N.;
RT "The germ cell-less gene product: a posteriorly localized component
RT necessary for germ cell development in Drosophila.";
RL Cell 70:569-584(1992).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Required for the specification of pole cells and germ cell
CC formation. Mothers with reduced glc function give rise to sterile adult
CC progeny that lack germ cells.
CC -!- SUBCELLULAR LOCATION: Cytoplasm. Note=Pole plasm.
CC -!- DEVELOPMENTAL STAGE: Oogenesis and embryogenesis.
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DR EMBL; M97933; AAA28566.1; -; Genomic_DNA.
DR EMBL; AE013599; AAF59048.1; -; Genomic_DNA.
DR EMBL; AY061319; AAL28867.1; -; mRNA.
DR PIR; A43317; A43317.
DR RefSeq; NP_724708.1; NM_165622.2.
DR AlphaFoldDB; Q01820; -.
DR SMR; Q01820; -.
DR BioGRID; 61712; 12.
DR DIP; DIP-21769N; -.
DR IntAct; Q01820; 3.
DR STRING; 7227.FBpp0087789; -.
DR PaxDb; Q01820; -.
DR DNASU; 35864; -.
DR EnsemblMetazoa; FBtr0088710; FBpp0087789; FBgn0005695.
DR GeneID; 35864; -.
DR KEGG; dme:Dmel_CG8411; -.
DR CTD; 35864; -.
DR FlyBase; FBgn0005695; gcl.
DR VEuPathDB; VectorBase:FBgn0005695; -.
DR eggNOG; KOG4682; Eukaryota.
DR GeneTree; ENSGT00940000168453; -.
DR InParanoid; Q01820; -.
DR PhylomeDB; Q01820; -.
DR SignaLink; Q01820; -.
DR BioGRID-ORCS; 35864; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35864; -.
DR PRO; PR:Q01820; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0005695; Expressed in egg cell and 45 other tissues.
DR ExpressionAtlas; Q01820; baseline and differential.
DR Genevisible; Q01820; DM.
DR GO; GO:0005938; C:cell cortex; IDA:FlyBase.
DR GO; GO:0031463; C:Cul3-RING ubiquitin ligase complex; IPI:FlyBase.
DR GO; GO:0031234; C:extrinsic component of cytoplasmic side of plasma membrane; IDA:FlyBase.
DR GO; GO:0005635; C:nuclear envelope; IDA:FlyBase.
DR GO; GO:0034399; C:nuclear periphery; IDA:FlyBase.
DR GO; GO:0005643; C:nuclear pore; IDA:FlyBase.
DR GO; GO:0005634; C:nucleus; TAS:FlyBase.
DR GO; GO:1990756; F:ubiquitin ligase-substrate adaptor activity; IDA:FlyBase.
DR GO; GO:0007281; P:germ cell development; IGI:FlyBase.
DR GO; GO:0120177; P:negative regulation of torso signaling pathway; IMP:FlyBase.
DR GO; GO:0016480; P:negative regulation of transcription by RNA polymerase III; IMP:FlyBase.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; TAS:FlyBase.
DR GO; GO:0007278; P:pole cell fate determination; IDA:FlyBase.
DR GO; GO:0007279; P:pole cell formation; TAS:FlyBase.
DR GO; GO:0043161; P:proteasome-mediated ubiquitin-dependent protein catabolic process; IDA:FlyBase.
DR GO; GO:0000209; P:protein polyubiquitination; IDA:FlyBase.
DR Gene3D; 3.30.710.10; -; 1.
DR InterPro; IPR000210; BTB/POZ_dom.
DR InterPro; IPR043380; Gcl-like.
DR InterPro; IPR011333; SKP1/BTB/POZ_sf.
DR PANTHER; PTHR23231; PTHR23231; 1.
DR Pfam; PF00651; BTB; 1.
DR SMART; SM00225; BTB; 1.
DR SUPFAM; SSF54695; SSF54695; 1.
DR PROSITE; PS50097; BTB; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Developmental protein; Reference proteome.
FT CHAIN 1..569
FT /note="Protein germ cell-less"
FT /id="PRO_0000087442"
FT DOMAIN 66..136
FT /note="BTB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00037"
FT REGION 17..43
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 517..553
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 569 AA; 65152 MW; A91A670C4B5F83A4 CRC64;
MGQIVGSMHM NVAEVFSNRR KRKRSTDSSL GKDDPAQLDT TQPKKKKLLT TTQYIYKALF
KEEKNSDVAV MALDKVWHLH KVYLSQSPYF YTMFNGTWRE AQQNFIQITI LDDRITVASL
DAVFGSMYSD EIEIESADVI SVLATATLFH LDGIIDKCAE VMVDNISPET AIQYYEAACQ
YGVVGVKKST FQWFQINLLS IYSKQPNLLR HISIELMSAL TASPDLYVMQ TEFSLYTLLR
TWMFLRLHPD YDPEDPVQRA EALKTQELLV NAGVETHAPS GDVVQWTYFT SRSEERSFLA
TPEGQPYVKV FQKLRTQYLT NHYMDLKIIY NDNIIPKEWL YRHIHNHWDA LLRIDHGQED
CSPQQLDDEQ FFENCMRCGR MLLEPGYQKW RWTGFNFGMD LILIMDSRRL NIRRHHRHEH
ERVLSLQTKR KFMVRTTVTS INAQRQAVFT QTSEICSLSL EKNEEVPLMV LDPKLVHPLL
ISINMLVVMP PNQSFKEIVP LSEEATTSLS IPISEIGANS DRPLSPSSAD DSAVFIGDSE
PSTPSSPAPR PRIAWSASET GAICGQLAC
