GCM2_DROME
ID GCM2_DROME Reviewed; 606 AA.
AC Q9VLA2; Q4V704; Q8N012; Q8WST6; Q9NCQ2;
DT 25-JUL-2003, integrated into UniProtKB/Swiss-Prot.
DT 11-JUN-2014, sequence version 4.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Transcription factor glial cells missing 2;
DE AltName: Full=Protein glide-2;
GN Name=gcm2; ORFNames=CG3858;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=11532931; DOI=10.1093/emboj/20.17.4664;
RA Kammerer M., Giangrande A.;
RT "Glide2, a second glial promoting factor in Drosophila melanogaster.";
RL EMBO J. 20:4664-4673(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Hosoya T., Iwasaki Y., Osato M., Higashi-Takizawa C., Akiyama-Oda Y.,
RA Ikenaka K., Hiromi Y., Hotta Y.;
RT "Binary cell-fate decisions in Drosophila neurogenesis and hematopoiesis
RT regulated by Gcm-motif transcription factors.";
RL Submitted (FEB-2001) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 5-606, FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12167411; DOI=10.1006/dbio.2002.0740;
RA Alfonso T.B., Jones B.W.;
RT "gcm2 promotes glial cell differentiation and is required with glial cells
RT missing for macrophage development in Drosophila.";
RL Dev. Biol. 248:369-383(2002).
CC -!- FUNCTION: Transcription factor with a minor role promoting glial cell
CC differentiation and a more significant role in hematocyte
CC differentiation. Gcm2, together with gcm, is required for the
CC proliferation of plasmatocyte precursors, the expression of Croquemort
CC protein, and the ability of plasmatocytes to convert into macrophages.
CC {ECO:0000269|PubMed:11532931, ECO:0000269|PubMed:12167411}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00245}.
CC -!- TISSUE SPECIFICITY: Expressed in glial lineages within embryonic
CC procephalic mesoderm. Expression is highest in hemocyte primordia and
CC longitudinal and nerve root ganglia. {ECO:0000269|PubMed:11532931,
CC ECO:0000269|PubMed:12167411}.
CC -!- DEVELOPMENTAL STAGE: First appears in embryos, levels decrease in
CC larvae and peak again in 1 day old pupae. No expression is found in
CC adults. {ECO:0000269|PubMed:11532931}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAM22408.1; Type=Miscellaneous discrepancy; Note=Intron retention.; Evidence={ECO:0000305};
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DR EMBL; AF184664; AAF74349.1; -; mRNA.
DR EMBL; AB056467; BAB83120.1; -; mRNA.
DR EMBL; AE014134; AAF52793.2; -; Genomic_DNA.
DR EMBL; BT022152; AAY51547.1; -; mRNA.
DR EMBL; AF461416; AAM22408.1; ALT_SEQ; mRNA.
DR RefSeq; NP_609302.1; NM_135458.5.
DR AlphaFoldDB; Q9VLA2; -.
DR SMR; Q9VLA2; -.
DR BioGRID; 60379; 31.
DR IntAct; Q9VLA2; 13.
DR STRING; 7227.FBpp0089223; -.
DR PaxDb; Q9VLA2; -.
DR DNASU; 34280; -.
DR EnsemblMetazoa; FBtr0079837; FBpp0079435; FBgn0019809.
DR GeneID; 34280; -.
DR KEGG; dme:Dmel_CG3858; -.
DR CTD; 9247; -.
DR FlyBase; FBgn0019809; gcm2.
DR VEuPathDB; VectorBase:FBgn0019809; -.
DR eggNOG; ENOG502QU2X; Eukaryota.
DR GeneTree; ENSGT00390000006777; -.
DR HOGENOM; CLU_031237_0_0_1; -.
DR InParanoid; Q9VLA2; -.
DR OMA; HCTLPNG; -.
DR OrthoDB; 722438at2759; -.
DR BioGRID-ORCS; 34280; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 34280; -.
DR PRO; PR:Q9VLA2; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0019809; Expressed in head mesoderm (Drosophila) and 22 other tissues.
DR Genevisible; Q9VLA2; DM.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0048813; P:dendrite morphogenesis; IMP:FlyBase.
DR GO; GO:0035165; P:embryonic crystal cell differentiation; IMP:FlyBase.
DR GO; GO:0021782; P:glial cell development; IMP:FlyBase.
DR GO; GO:0010001; P:glial cell differentiation; IMP:UniProtKB.
DR GO; GO:0042063; P:gliogenesis; IBA:GO_Central.
DR GO; GO:0042386; P:hemocyte differentiation; IEP:UniProtKB.
DR GO; GO:0042690; P:negative regulation of crystal cell differentiation; IMP:FlyBase.
DR GO; GO:0030182; P:neuron differentiation; IMP:FlyBase.
DR GO; GO:0042387; P:plasmatocyte differentiation; IEP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 2.20.25.670; -; 1.
DR Gene3D; 3.30.70.3530; -; 1.
DR InterPro; IPR039791; GCM.
DR InterPro; IPR036115; GCM_dom_sf.
DR InterPro; IPR043020; GCM_large.
DR InterPro; IPR043021; GCM_small.
DR InterPro; IPR003902; Tscrpt_reg_GCM.
DR PANTHER; PTHR12414; PTHR12414; 1.
DR Pfam; PF03615; GCM; 1.
DR SUPFAM; SSF90073; SSF90073; 1.
DR PROSITE; PS50807; GCM; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; DNA-binding; Nucleus; Reference proteome;
KW Transcription; Transcription regulation.
FT CHAIN 1..606
FT /note="Transcription factor glial cells missing 2"
FT /id="PRO_0000126653"
FT DNA_BIND 65..224
FT /note="GCM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00245"
FT REGION 20..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 475..501
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 561..606
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 20..63
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..501
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 566..581
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 32
FT /note="S -> N (in Ref. 2; BAB83120)"
FT /evidence="ECO:0000305"
FT CONFLICT 37
FT /note="S -> P (in Ref. 2; BAB83120 and 6; AAM22408)"
FT /evidence="ECO:0000305"
FT CONFLICT 41
FT /note="G -> C (in Ref. 2; BAB83120)"
FT /evidence="ECO:0000305"
FT CONFLICT 50
FT /note="G -> V (in Ref. 6; AAM22408)"
FT /evidence="ECO:0000305"
FT CONFLICT 60
FT /note="T -> A (in Ref. 2; BAB83120)"
FT /evidence="ECO:0000305"
FT CONFLICT 288
FT /note="A -> T (in Ref. 6; AAM22408)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="S -> N (in Ref. 2; BAB83120)"
FT /evidence="ECO:0000305"
FT CONFLICT 433
FT /note="G -> S (in Ref. 6; AAM22408)"
FT /evidence="ECO:0000305"
FT CONFLICT 489
FT /note="Q -> E (in Ref. 6; AAM22408)"
FT /evidence="ECO:0000305"
FT CONFLICT 543
FT /note="S -> N (in Ref. 2; BAB83120)"
FT /evidence="ECO:0000305"
FT CONFLICT 564
FT /note="T -> A (in Ref. 2; BAB83120 and 6; AAM22408)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 606 AA; 65674 MW; C5DF4B3C4ADE8478 CRC64;
MVVINGYSFK TQQLLTQQPD HSQLTQFVQP QSQSTHSVHP GPSPGQQQAG GSMTMPSSST
GKGKREWDIN DAIVPHVPDQ EFDEFNEWSD GHVRHIYSLH NEEAKKHISG WAMRNTNNHN
VNILKKSCLG VLVCSQHCTL PNGSKINLRP AICDKARRKQ EGKACPNKSC RGGRLEIKPC
RGHCGYPVTH FWRHSGNAIF FQAKGVHDHL RPDPKNSSVS KRAFGRVPLA GKSANGSVAK
KSVIAGLVKQ AKQQHSLISK VLKRPAVSNP LAHTALDIYQ YNACGKCAGY SHCTCSYLDD
STTARSHQLS QSSNYGTNSW PLSGSESSAP CETAANVFTV NHQHITYNYP IYHATPAAAT
AAPSKSPSLP YACSISELAA YQQSSSGNSF AMGVPVHGHT QCQAVAYDSS PQLATPEPEF
INYSQIKHLG GGGGQEEISC KAEPGPTIKY NATVETQPYV EDNYDYYYSP KAEYEMQQHH
HQQQQSHQQF GGNQTAGHHY YESSSGYNGV SYFDTGTTTA PGNTATGNGL EVGYGGYYDH
YTSYEQQMAV AGGFATAGGS TAPTVAAPPG HPPPPPPPPT LTYHHHHHHH LHHPAAATGL
APSVTH
