GCN1_DICDI
ID GCN1_DICDI Reviewed; 2667 AA.
AC Q54WR2;
DT 29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 86.
DE RecName: Full=eIF-2-alpha kinase activator GCN1 {ECO:0000250|UniProtKB:E9PVA8};
DE AltName: Full=GCN1-like protein 1 {ECO:0000250|UniProtKB:E9PVA8};
DE AltName: Full=Translational activator gcn1 {ECO:0000250|UniProtKB:E9PVA8};
GN Name=gcn1 {ECO:0000250|UniProtKB:E9PVA8}; Synonyms=gcn1l1;
GN ORFNames=DDB_G0279487;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Acts as a positive activator of the gcn2 protein kinase
CC activity in response to amino acid starvation. Forms a complex with
CC eif2ak4/gcn2 on translating ribosomes; during this process, gcn1 seems
CC to act as a chaperone to facilitate delivery of uncharged tRNAs that
CC enter the A site of ribosomes to the tRNA-binding domain of
CC eif2ak4/gcn2, and hence stimulating eif2ak4/gcn2 kinase activity.
CC Participates in the repression of global protein synthesis and in gene-
CC specific mRNA translation activation, such as the transcriptional
CC activator atf4, by promoting the eif2ak4/gcn2-mediated phosphorylation
CC of eukaryotic translation initiation factor 2 (eIF-2-alpha/eif2s1) on
CC 'Ser-53', and hence allowing atf4-mediated reprogramming of amino acid
CC biosynthetic gene expression to alleviate nutrient depletion.
CC {ECO:0000250|UniProtKB:E9PVA8, ECO:0000250|UniProtKB:P33892}.
CC -!- SUBUNIT: Interacts with eif2ak4/gcn2; this interaction stimulates the
CC eif2ak4/gcn2 kinase activity and is impaired by impact upon a variety
CC of stress conditions, such as amino acid depletion, UV-C irradiation,
CC proteasome inhibitor treatment and glucose deprivation. Interacts with
CC impact; this prevents the interaction of gcn1 with eif2ak4/gcn2 and
CC inhibits eif2ak4/gcn2 kinase activity. {ECO:0000250|UniProtKB:E9PVA8}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:E9PVA8}.
CC -!- SIMILARITY: Belongs to the GCN1 family. {ECO:0000305}.
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DR EMBL; AAFI02000031; EAL67682.1; -; Genomic_DNA.
DR RefSeq; XP_641655.1; XM_636563.1.
DR AlphaFoldDB; Q54WR2; -.
DR STRING; 44689.DDB0234065; -.
DR PaxDb; Q54WR2; -.
DR EnsemblProtists; EAL67682; EAL67682; DDB_G0279487.
DR GeneID; 8622062; -.
DR KEGG; ddi:DDB_G0279487; -.
DR dictyBase; DDB_G0279487; -.
DR eggNOG; KOG1242; Eukaryota.
DR HOGENOM; CLU_000504_2_0_1; -.
DR InParanoid; Q54WR2; -.
DR OMA; ASMKIFL; -.
DR PhylomeDB; Q54WR2; -.
DR PRO; PR:Q54WR2; -.
DR Proteomes; UP000002195; Chromosome 3.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0019901; F:protein kinase binding; IEA:InterPro.
DR GO; GO:0019887; F:protein kinase regulator activity; IBA:GO_Central.
DR GO; GO:0043022; F:ribosome binding; ISS:UniProtKB.
DR GO; GO:0034198; P:cellular response to amino acid starvation; ISS:UniProtKB.
DR GO; GO:0072755; P:cellular response to benomyl; ISS:UniProtKB.
DR GO; GO:0070301; P:cellular response to hydrogen peroxide; ISS:UniProtKB.
DR GO; GO:1990451; P:cellular stress response to acidic pH; ISS:UniProtKB.
DR GO; GO:0033674; P:positive regulation of kinase activity; IEA:InterPro.
DR GO; GO:0071264; P:positive regulation of translational initiation in response to starvation; ISS:UniProtKB.
DR GO; GO:0006417; P:regulation of translation; IBA:GO_Central.
DR GO; GO:0006448; P:regulation of translational elongation; ISS:dictyBase.
DR Gene3D; 1.25.10.10; -; 6.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR033173; Gcn1.
DR InterPro; IPR000357; HEAT.
DR InterPro; IPR021133; HEAT_type_2.
DR InterPro; IPR034085; TOG.
DR PANTHER; PTHR23346:SF7; PTHR23346:SF7; 1.
DR Pfam; PF02985; HEAT; 1.
DR SMART; SM01349; TOG; 2.
DR SUPFAM; SSF48371; SSF48371; 3.
DR PROSITE; PS50077; HEAT_REPEAT; 3.
PE 3: Inferred from homology;
KW Activator; Cytoplasm; Reference proteome; Repeat; Stress response;
KW Translation regulation.
FT CHAIN 1..2667
FT /note="eIF-2-alpha kinase activator GCN1"
FT /id="PRO_0000330848"
FT REPEAT 19..56
FT /note="HEAT 1"
FT /evidence="ECO:0000255"
FT REPEAT 95..132
FT /note="HEAT 2"
FT /evidence="ECO:0000255"
FT REPEAT 159..198
FT /note="HEAT 3"
FT /evidence="ECO:0000255"
FT REPEAT 293..330
FT /note="HEAT 4"
FT /evidence="ECO:0000255"
FT REPEAT 336..375
FT /note="HEAT 5"
FT /evidence="ECO:0000255"
FT REPEAT 398..439
FT /note="HEAT 6"
FT /evidence="ECO:0000255"
FT REPEAT 466..503
FT /note="HEAT 7"
FT /evidence="ECO:0000255"
FT REPEAT 794..832
FT /note="HEAT 8"
FT /evidence="ECO:0000255"
FT REPEAT 929..967
FT /note="HEAT 9"
FT /evidence="ECO:0000255"
FT REPEAT 985..1024
FT /note="HEAT 10"
FT /evidence="ECO:0000255"
FT REPEAT 1085..1122
FT /note="HEAT 11"
FT /evidence="ECO:0000255"
FT REPEAT 1199..1237
FT /note="HEAT 12"
FT /evidence="ECO:0000255"
FT REPEAT 1290..1330
FT /note="HEAT 13"
FT /evidence="ECO:0000255"
FT REPEAT 1333..1370
FT /note="HEAT 14"
FT /evidence="ECO:0000255"
FT REPEAT 1371..1407
FT /note="HEAT 15"
FT /evidence="ECO:0000255"
FT REPEAT 1412..1450
FT /note="HEAT 16"
FT /evidence="ECO:0000255"
FT REPEAT 1454..1491
FT /note="HEAT 17"
FT /evidence="ECO:0000255"
FT REPEAT 1492..1529
FT /note="HEAT 18"
FT /evidence="ECO:0000255"
FT REPEAT 1533..1570
FT /note="HEAT 19"
FT /evidence="ECO:0000255"
FT REPEAT 1572..1608
FT /note="HEAT 20"
FT /evidence="ECO:0000255"
FT REPEAT 1610..1647
FT /note="HEAT 21"
FT /evidence="ECO:0000255"
FT REPEAT 1652..1689
FT /note="HEAT 22"
FT /evidence="ECO:0000255"
FT REPEAT 1691..1728
FT /note="HEAT 23"
FT /evidence="ECO:0000255"
FT REPEAT 1729..1766
FT /note="HEAT 24"
FT /evidence="ECO:0000255"
FT REPEAT 1770..1807
FT /note="HEAT 25"
FT /evidence="ECO:0000255"
FT REPEAT 1809..1845
FT /note="HEAT 26"
FT /evidence="ECO:0000255"
FT REPEAT 1882..1919
FT /note="HEAT 27"
FT /evidence="ECO:0000255"
FT REPEAT 1923..1960
FT /note="HEAT 28"
FT /evidence="ECO:0000255"
FT REPEAT 1962..1998
FT /note="HEAT 29"
FT /evidence="ECO:0000255"
FT REPEAT 2002..2039
FT /note="HEAT 30"
FT /evidence="ECO:0000255"
FT REPEAT 2040..2078
FT /note="HEAT 31"
FT /evidence="ECO:0000255"
FT REPEAT 2080..2110
FT /note="HEAT 32"
FT /evidence="ECO:0000255"
FT REPEAT 2114..2152
FT /note="HEAT 33"
FT /evidence="ECO:0000255"
FT REPEAT 2154..2190
FT /note="HEAT 34"
FT /evidence="ECO:0000255"
FT REPEAT 2193..2230
FT /note="HEAT 35"
FT /evidence="ECO:0000255"
FT REPEAT 2264..2301
FT /note="HEAT 36"
FT /evidence="ECO:0000255"
FT REPEAT 2326..2348
FT /note="HEAT 37; degenerate"
FT /evidence="ECO:0000255"
FT REPEAT 2349..2385
FT /note="HEAT 38; degenerate"
FT /evidence="ECO:0000255"
FT REPEAT 2387..2421
FT /note="HEAT 39"
FT /evidence="ECO:0000255"
FT REPEAT 2425..2462
FT /note="HEAT 40"
FT /evidence="ECO:0000255"
FT REPEAT 2508..2545
FT /note="HEAT 41"
FT /evidence="ECO:0000255"
FT REPEAT 2546..2583
FT /note="HEAT 42"
FT /evidence="ECO:0000255"
FT REGION 842..879
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1853..1875
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2667 AA; 298586 MW; A6EF48C268396F4D CRC64;
MNEENPSSSI ILDDQQQQDS FYSEIKELEK SIQSNKLSER KNTLTRINSI GKHELSTQEN
DATTTTTTTT VDLEEKLKVL IYLYFTSYSI GPDSQWIFSL VQSLKQLFKD ISSATNTSLV
TDELKINIVK FTLREIGRLA KLLPTNKRSK YVPQNTLMSF SLVLLNYFID QIQSNSDLTT
LLFAAQELLY RELTLQHMKN NQPIFNQILN KNKILTFYQN IVTKDSSNKS FFIVYFLLRN
FTDQSNKQKE QDNTFKINDL LNIYNKTVIG SSQQKIEEHK FFKRLFNQLT NEDLQSIILP
PLSRHIKRDQ DQVFKILIFI LENLSSDFNV IDLSSLLKSM LLPMLLPVIQ STISIEENRK
LLKKTFTLII ERSKDTKLIS SMITDDLLKT LSVAGNPSQK LIIISIISSI ISTKNFIERL
SLTTEKLQLS KQILQSISIY LEKELNKDNR NKGFKLLGKV MKMVEELPEQ TIKIITNSLK
NDDDIIKGQV ILSLSKSLGP EANGTNKKVI QIINGFTETI NTILKNVKNA KTCDPSTTTA
SLHYMLSLIT TTGVPKNDIF TKYSTDKTTI SNLYASTSFL HTDGFIQRTS KKDHAIDLLL
TLFLRVKSFP SIKLNDKSPL YSSVLNCLLH SQWSVSKHSA IKIRSILSRN DSVDIDYPLL
SNQLLIEFST ILFDDSLIIT PQIINSNVES TTTTTTTISN KKNYLIAFRS ILSKNIKSEL
YPMLSLICYH PFINYNWKRV SSLIQNDVNT TLSSNAIEIS KYIFEKGLNQ KKNKSYQQAF
QQAINGLMNY NVPLLNEELV KLMVKALSYE PVLAITQQQW SIYHTLPTEL FVEKQEQLVE
SRNDRKVKPK TAEEQRDEES RKRIEEKKKI QSGELEKQEK ERQKQLAAQA VIRKDVQDVI
DRLHLAMDTC QTMAKSSSNP QFVGEFMSPI IVALLQLMKH EITNHQFTQV FEKLICCVPS
RFKLDRSFAR HYIYIINNIY YRPTLSEIQI LGFIQKILTH IRESIAKEAL SGFAFNYFWP
IIKNGLETTI SFTIQEISME IIQKHTAQGQ AYPRGSMISS LIIVVSTNSR LEAQARNTIF
QLIEGVETSD IGELMEGIIS KHVQVRSICL QAIEKIPSIY SPSFVWEDKY IGSLWFARFD
NHDANTSALA EKIWLATNQP TQLPEDFMKL LSDSTFNVNS ETRKINALAI KEAATCHTHM
IPEIVDNLFE IYEQNYPDEI RETPITSKFR ISVATALSGL GNAIVEPEVL KSLFTKIIER
GLFDPKEEVV QEFVSTGMSI INQQGVQFSG ELLATFEAFL ARPDNGTGEE DSIRANVVVY
MGALAKHMDA SNPKVSIVID KLVDALSIPS ESVQVGISKC IAQLIPSFKK QGDRLIPMLL
EKLKNSSGNY ADRRGAAFGL AGSVKGLGIG SLKNYSILDT LQSYIEDKKH PTSRQGALFA
FECLCNTIGR VFEPYIIHIL PKLLVCFGDN VSEVRDATAD TAKAIMSQLS GHGVKIVLPA
LLKALDDRSW RTKEGSIELL GAMAFCAPKQ LSSCLPTIVP KLTYVLNDTH TKVQEAAKEA
LSHIGSVIRN PEIQIHVPLL LQTYDDPEIH SKELLENLLS TNYVHTIDPA SLSLLLPILE
RTLKERSSEL KKMSCQIVGN LCSLTEPKDL VPYLNILMPV MKTVLLDPIP EVRAICARAL
GLLVRGMGEE NFSTLIPWLL ETVKSDQGAV ERSGAAQGLS EVLASLDISR FNSLINELLA
MTNSPRPHVR EGILSIFIFT PISLGDLFLP YLPKVLPQVL KGLADDSDPV REVCMRCGQS
IVLQFAVTGI EVIVPALEKV LFHENWRIRL SCVQLFGDLL FKLAGTTAQE VQSNNSSYNA
KDDDDDEPGS SGNDIQKILG KERLGRILSS LYMMRFDNNS SVRQKVLLIW KYIVSNTPKT
LREILPTLIE MIISSIGSNN VEKRQISAKT LGDIVSKLSD RILPEILPIL ERGLRSELEE
TRQGVCIGLS EVISSAKTQL LPYLSSVVTC ITKALCDPLI DVREAAAKAF DHLYHTFGSK
ASNEILPQLI QLLDNSNNKD LAGYALDGLR QVILVRSSIV LPVLIPKLLS RPISTSNVTA
LSSLAADAGE GLYVHLSTII PSLIESFTNP NTISNAKEIK EAAVSICKSI DEQGWDTLIG
LLIEQTEIRL PNIRLGACEL IGEFYNGNTM VTEYPEELLL SLLSLFNDPD ALVQQAANNA
LGFITKSLKK DNLTYLPVFQ KGIQLLVNET YEEVSTIPGF CLPKGLASVL PVLISGLMYG
TSDQREQATN TLRTVINHTS ADALKPFVMQ ITGPLILVIG DKFPWQVKSA ILQTLSLLIS
KSPASMKIFL HQLQPTFIKC LSDSHKNVRT NAASALGLLM TLSSSVDQLV NSLITGISTA
DSISQESKLR ALQSIFEKKP KVEQATLDKA IATIVDFLYQ PSDDLRSMVA QTIGASSKCF
TSLTELNQFI KTNLISPSQS VLSRYGKSLA LGEIFKASGK NLIDSQSPNM PTIIKIIQTD
CRDEKGPIRE SSAYLAEAIL VASPLTYAKD LVPSICHLIG DQSSSVSISA LNVIKRFCKS
NQQLSRQYLR DIVVPTMNRL KERTNLPLKL AAERTLVHSL QIFKESIVMD DLIKQLELSG
DSSMANSLID YHKRVLMKLS PDSDIEK
