GCNA_DROME
ID GCNA_DROME Reviewed; 1030 AA.
AC Q7KW09;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Germ cell nuclear acidic protein {ECO:0000305};
DE AltName: Full=Germ cell nuclear acidic peptidase {ECO:0000303|PubMed:31839537};
GN Name=Gcna {ECO:0000312|FlyBase:FBgn0023515};
GN ORFNames=CG14814 {ECO:0000312|FlyBase:FBgn0023515};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP DISRUPTION PHENOTYPE, FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=31839537; DOI=10.1016/j.devcel.2019.11.007;
RA Bhargava V., Goldstein C.D., Russell L., Xu L., Ahmed M., Li W., Casey A.,
RA Servage K., Kollipara R., Picciarelli Z., Kittler R., Yatsenko A.,
RA Carmell M., Orth K., Amatruda J.F., Yanowitz J.L., Buszczak M.;
RT "GCNA Preserves Genome Integrity and Fertility Across Species.";
RL Dev. Cell 52:38-52(2020).
CC -!- FUNCTION: May play a role in DNA-protein cross-links (DPCs) clearance,
CC ensuring the genomic stability by protecting germ cells and early
CC embryos from various sources of damage (PubMed:31839537). Limits
CC replication stress and DNA double-strand breaks (PubMed:31839537).
CC {ECO:0000269|PubMed:31839537}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:31839537}.
CC Chromosome {ECO:0000269|PubMed:31839537}. Note=During mitosis,
CC associated with dividing chromosomes. {ECO:0000269|PubMed:31839537}.
CC -!- DISRUPTION PHENOTYPE: Homozygous mutant females initially laid eggs but
CC stopped after approximately one week. Many of the embryos derived from
CC these eggs exhibited maternal-effect lethality (PubMed:31839537). The
CC few adults from F1 progeny of homozygous mutant females appear sickly
CC and sub-fertile. Of these, 4% display bilateral gynandromorphism a rare
CC phenotype caused by X chromosome loss during the early embryogenesis.
CC By contrast, embryos from homozygous mutant females displayed X
CC chromosome bridges and second chromosome missegregation and polyploidy
CC (PubMed:31839537). {ECO:0000269|PubMed:31839537}.
CC -!- SIMILARITY: Belongs to the serine-aspartate repeat-containing protein
CC (SDr) family. {ECO:0000305}.
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DR EMBL; AE014298; AAN09061.2; -; Genomic_DNA.
DR EMBL; AE014298; AHN59264.1; -; Genomic_DNA.
DR RefSeq; NP_001284793.1; NM_001297864.1.
DR RefSeq; NP_726770.2; NM_166909.3.
DR IntAct; Q7KW09; 1.
DR EnsemblMetazoa; FBtr0070370; FBpp0070354; FBgn0023515.
DR EnsemblMetazoa; FBtr0339494; FBpp0308578; FBgn0023515.
DR GeneID; 31139; -.
DR KEGG; dme:Dmel_CG14814; -.
DR UCSC; CG14814-RA; d. melanogaster.
DR FlyBase; FBgn0023515; Gcna.
DR VEuPathDB; VectorBase:FBgn0023515; -.
DR GeneTree; ENSGT00440000040163; -.
DR OrthoDB; 294087at2759; -.
DR BioGRID-ORCS; 31139; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 31139; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0023515; Expressed in egg chamber and 30 other tissues.
DR ExpressionAtlas; Q7KW09; baseline and differential.
DR GO; GO:0005694; C:chromosome; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0044547; F:DNA topoisomerase binding; IDA:UniProtKB.
DR GO; GO:1904931; F:MCM complex binding; IDA:UniProtKB.
DR GO; GO:0106300; P:protein-DNA covalent cross-linking repair; IMP:UniProtKB.
DR InterPro; IPR006640; SprT-like_domain.
DR InterPro; IPR035240; SprT_Zn_ribbon.
DR Pfam; PF10263; SprT-like; 1.
DR Pfam; PF17283; Zn_ribbon_SprT; 1.
DR SMART; SM00731; SprT; 1.
PE 3: Inferred from homology;
KW Chromosome; Cytoplasm; Reference proteome.
FT CHAIN 1..1030
FT /note="Germ cell nuclear acidic protein"
FT /id="PRO_0000454375"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 196..245
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 266..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 375..433
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 457..594
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 699..764
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 17..35
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 202..245
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 279..319
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 330..361
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 502..520
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 526..557
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 574..594
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 725..739
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1030 AA; 114277 MW; 59BF67517CC26B81 CRC64;
MADHKYRLFN AVDPSKVSHA PKDHPEKRND QKTRKLPAAP MRANVGAPAP SCGANTEDTE
LDNSDYMEAL QLSADGCSQD VANVGDVELL SAGISNLKVQ PINEEHSAKP EISICISSTT
EENVSGDDDA ASCITISDSS EDEQEPLPPD SMAVSEREIV ANPGQEDIPQ PMLTTEKVQR
IEAFLRDVSI ERREMEWNGP LTPPPLSSSQ RQSRLADADT ESMSPTDEDW QPPTTRPIYN
STQLANNDTQ VNTICSADAT RLDSSKRLAD DETEANTLGS EELEPTYSSK RLADNDTEPN
TLCSDSSETH NSTIHNNEPV PDESIEIPET SSEGELTPQK ASSGSSTSGG DEHGSIQVSS
INISAKINIK ISIPTMESSS AEDDDEHYSS PRATKSLPSS EEVQHQEHQQ QDPQSHQKSM
LSVDDASEDE QFLTHAEKLL NQLYGKSWQT PDVIRTLKRS SGSGGKQAPL RPRNIQATAV
TTAKKKKPKP DESVLGDFSI FTKPLRTNQT PLNSTRLPPM RAVQTERRPR TQKPLTRPRT
NHIDEDRWRK LIDSDSGTDA SDDEDADATF SESGSDSDSD KDKENKQKRG DLTYLDLSKD
EVEVISNPDQ DSPSPKCHRR LDDILRSCRA SVKAKLPATP VQQPNIRRQL FTPNAGFEND
NQAKEIVNRA LDLDMLDELE SDYLPGTPVH RRVQEVKKKL GIGQQGQTTP HESPILKLLT
PKTAPPKGTA PPKTSAPPKV STPPKSTKQI PKPRKQKDLS PVASGGKCGF LKSLEGQVSR
DRADNEAFFY RENFARNKDQ LAQHLYKMYN AQVFNNELDV PITWSKLLRN TAGRCMNKRK
LNQRSSVVEL SVKVLTTADR LRCTLIHELC HAAAWVFNGE GGHGRVWKMW AQRANDKFPD
LPQIKVCHNY SIEFKYTYKC LSCDKASHAH SRSRKVEDLR CRICRGPITL FLNKKDKQGN
TVSTPAGEAK GFAKFVKDNF QRHKRDNMTA AQVMRILSVE YAKQKGQPAE ETAASIASRV
ETLTLDESSN
