GCNA_MOUSE
ID GCNA_MOUSE Reviewed; 507 AA.
AC A0A1D9BZF0;
DT 23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT 18-JAN-2017, sequence version 1.
DT 03-AUG-2022, entry version 10.
DE RecName: Full=Germ cell nuclear acidic protein {ECO:0000305};
DE AltName: Full=Acidic repeat-containing protein {ECO:0000250|UniProtKB:Q96QF7};
DE AltName: Full=Germ cell nuclear antigen {ECO:0000303|PubMed:27718356};
GN Name=Gcna {ECO:0000312|MGI:MGI:105113};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=C57BL/6J;
RX PubMed=27718356; DOI=10.7554/elife.19993;
RA Carmell M.A., Dokshin G.A., Skaletsky H., Hu Y.C., van Wolfswinkel J.C.,
RA Igarashi K.J., Bellott D.W., Nefedov M., Reddien P.W., Enders G.C.,
RA Uversky V.N., Mello C.C., Page D.C.;
RT "A widely employed germ cell marker is an ancient disordered protein with
RT reproductive functions in diverse eukaryotes.";
RL Elife 5:0-0(2016).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP SUBCELLULAR LOCATION, INTERACTION WITH TOP2A, DISRUPTION PHENOTYPE, AND
RP FUNCTION.
RX PubMed=31839538; DOI=10.1016/j.devcel.2019.11.006;
RA Dokshin G.A., Davis G.M., Sawle A.D., Eldridge M.D., Nicholls P.K.,
RA Gourley T.E., Romer K.A., Molesworth L.W., Tatnell H.R., Ozturk A.R.,
RA de Rooij D.G., Hannon G.J., Page D.C., Mello C.C., Carmell M.A.;
RT "GCNA Interacts with Spartan and Topoisomerase II to Regulate Genome
RT Stability.";
RL Dev. Cell 52:53-68(2020).
CC -!- FUNCTION: May play a role in DNA-protein cross-links (DPCs) clearance
CC through a SUMO-dependent recruitment to sites of DPCs, ensuring the
CC genomic stability by protecting germ cells and early embryos from
CC various sources of damage (PubMed:31839538). Can resolve the
CC topoisomerase II (TOP2A) DPCs (PubMed:31839538).
CC {ECO:0000269|PubMed:31839538}.
CC -!- SUBUNIT: Interacts (via SIM domains) with SUMO2; this interaction
CC allows the GCNA recruitment to DPCs sites (By similarity). Interacts
CC with TOP2A; this interaction allows the resolution of topoisomerase II
CC (TOP2A) DNA-protein cross-links (PubMed:31839538).
CC {ECO:0000250|UniProtKB:Q96QF7, ECO:0000269|PubMed:31839538}.
CC -!- SUBCELLULAR LOCATION: Chromosome {ECO:0000269|PubMed:31839538}.
CC Nucleus, PML body {ECO:0000269|PubMed:31839538}. Nucleus
CC {ECO:0000269|PubMed:27718356}. Note=Co-localizes with SUMO2 at PML
CC bodies in all interphase cells (By similarity). Localizes on condensed
CC chromosomes in spermatocytes in G2 and M during meiotic prophase
CC (PubMed:31839538). {ECO:0000250|UniProtKB:Q96QF7,
CC ECO:0000269|PubMed:31839538}.
CC -!- TISSUE SPECIFICITY: Germ-cells specific. {ECO:0000269|PubMed:27718356}.
CC -!- DOMAIN: SUMO interaction motif 1 (SIM) mediates the binding to
CC polysumoylated substrates. {ECO:0000250|UniProtKB:Q96QF7}.
CC -!- DISRUPTION PHENOTYPE: Mutant male mice are sterile and spermatocytes
CC exhibit DNA damage, crossover defects, and chromatin condensation
CC abnormalities. {ECO:0000269|PubMed:27718356,
CC ECO:0000269|PubMed:31839538}.
CC -!- SIMILARITY: Belongs to the serine-aspartate repeat-containing protein
CC (SDr) family. {ECO:0000305}.
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DR EMBL; KX981576; AOY07794.1; -; mRNA.
DR EMBL; AL805980; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR MGI; MGI:105113; Gcna.
DR Proteomes; UP000000589; Unplaced.
DR GO; GO:0000793; C:condensed chromosome; IDA:UniProtKB.
DR GO; GO:0016605; C:PML body; IDA:UniProtKB.
DR GO; GO:0106300; P:protein-DNA covalent cross-linking repair; IMP:UniProtKB.
PE 1: Evidence at protein level;
KW Chromosome; Nucleus; Reference proteome.
FT CHAIN 1..507
FT /note="Germ cell nuclear acidic protein"
FT /id="PRO_0000454372"
FT REGION 1..507
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 12..15
FT /note="SUMO interaction motif 1 (SIM)"
FT /evidence="ECO:0000250|UniProtKB:Q96QF7"
FT MOTIF 66..69
FT /note="SUMO interaction motif 1 (SIM)"
FT /evidence="ECO:0000250|UniProtKB:Q96QF7"
FT MOTIF 86..89
FT /note="SUMO interaction motif 1 (SIM)"
FT /evidence="ECO:0000250|UniProtKB:Q96QF7"
FT MOTIF 108..111
FT /note="SUMO interaction motif 1 (SIM)"
FT /evidence="ECO:0000250|UniProtKB:Q96QF7"
FT COMPBIAS 1..55
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 109..136
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 175..358
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 388..413
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 414..446
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 478..507
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 507 AA; 53394 MW; 02F8C91C30C38E6C CRC64;
MDSGSSSSSS SSGSSSGSCS TSGSGSTSGS STTSSSSSSS SSSSSSSSSS SKEYMPELPK
QRKASCVVID SESDSDNTSD EKNTTVCEIS SGDETSDIDR PGGQKLPLIV IDDDDDGSPD
LKNTKQKSDE PQMSVLEKEG VECIGSDSTS PHDVCEIWDV CGSSNQTSSE LEPEGEPESE
AKGEPESEAK GEPESEAKGE PESEAKGESE SEAKGEMETE AKGESESEAK GEMETEAKGE
SESEAKGEME TEAKGEPESE AKGEMETEAK GEPESEAKGE METEAKGESE SEAKGEMETE
AKGESESEAK GEMETEAKGE PESEAKGEME TEAKGEPESE AKGEPEPEAA KGEMETEAAM
GEVETEAAMG EPEQEITAEE AKKKRAAYLL AQQRKRKRKN RFICMSSSKP RRKRRRADPQ
DGADPQDGAD PQDRADPQDL ADPQDRGDSQ DMPSLPGTSD EPIPSGQPVC PRKGMASSRG
RGRGRGRGRG RGRGRGRGRG RGAKAGK
