GCNT7_MOUSE
ID GCNT7_MOUSE Reviewed; 433 AA.
AC Q3V3K7; B2RSZ6; Q3V174;
DT 21-AUG-2007, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 1.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-1,6-N-acetylglucosaminyltransferase 7;
DE EC=2.4.1.-;
GN Name=Gcnt7;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND NUCLEOTIDE SEQUENCE
RP [LARGE SCALE MRNA] OF 55-433 (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Head, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Glycosyltransferase. {ECO:0000250}.
CC -!- PATHWAY: Protein modification; protein glycosylation.
CC -!- SUBCELLULAR LOCATION: Golgi apparatus membrane {ECO:0000250}; Single-
CC pass type II membrane protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q3V3K7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q3V3K7-2; Sequence=VSP_027537;
CC -!- SIMILARITY: Belongs to the glycosyltransferase 14 family.
CC {ECO:0000305}.
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DR EMBL; AK039414; BAE20545.1; -; mRNA.
DR EMBL; AK132647; BAE21278.1; -; mRNA.
DR EMBL; AL833787; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC139073; AAI39074.1; -; mRNA.
DR EMBL; BC139076; AAI39077.1; -; mRNA.
DR CCDS; CCDS17133.1; -. [Q3V3K7-1]
DR RefSeq; NP_001034649.1; NM_001039560.3. [Q3V3K7-1]
DR RefSeq; XP_006500059.1; XM_006499996.3. [Q3V3K7-1]
DR AlphaFoldDB; Q3V3K7; -.
DR SMR; Q3V3K7; -.
DR STRING; 10090.ENSMUSP00000096659; -.
DR CAZy; GT14; Glycosyltransferase Family 14.
DR GlyGen; Q3V3K7; 1 site.
DR PhosphoSitePlus; Q3V3K7; -.
DR MaxQB; Q3V3K7; -.
DR PaxDb; Q3V3K7; -.
DR PRIDE; Q3V3K7; -.
DR Antibodypedia; 59236; 157 antibodies from 23 providers.
DR Ensembl; ENSMUST00000099060; ENSMUSP00000096659; ENSMUSG00000074569. [Q3V3K7-1]
DR GeneID; 654821; -.
DR KEGG; mmu:654821; -.
DR UCSC; uc008ocx.1; mouse. [Q3V3K7-1]
DR UCSC; uc012cko.1; mouse. [Q3V3K7-2]
DR CTD; 140687; -.
DR MGI; MGI:3606143; Gcnt7.
DR VEuPathDB; HostDB:ENSMUSG00000074569; -.
DR eggNOG; KOG0799; Eukaryota.
DR GeneTree; ENSGT00940000160402; -.
DR InParanoid; Q3V3K7; -.
DR OMA; LHTPGNC; -.
DR PhylomeDB; Q3V3K7; -.
DR TreeFam; TF315534; -.
DR Reactome; R-MMU-913709; O-linked glycosylation of mucins.
DR UniPathway; UPA00378; -.
DR BioGRID-ORCS; 654821; 3 hits in 72 CRISPR screens.
DR PRO; PR:Q3V3K7; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q3V3K7; protein.
DR Bgee; ENSMUSG00000074569; Expressed in olfactory epithelium and 18 other tissues.
DR ExpressionAtlas; Q3V3K7; baseline and differential.
DR Genevisible; Q3V3K7; MM.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0008375; F:acetylglucosaminyltransferase activity; IBA:GO_Central.
DR GO; GO:0006486; P:protein glycosylation; IEA:UniProtKB-UniPathway.
DR InterPro; IPR003406; Glyco_trans_14.
DR Pfam; PF02485; Branch; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Disulfide bond; Glycoprotein; Glycosyltransferase;
KW Golgi apparatus; Membrane; Reference proteome; Signal-anchor; Transferase;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..433
FT /note="Beta-1,3-galactosyl-O-glycosyl-glycoprotein beta-
FT 1,6-N-acetylglucosaminyltransferase 7"
FT /id="PRO_0000299039"
FT TOPO_DOM 1..8
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 9..25
FT /note="Helical; Signal-anchor for type II membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 26..433
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT REGION 233..275
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 413..433
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 254..275
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 112
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 57..209
FT /evidence="ECO:0000250"
FT DISULFID 143..358
FT /evidence="ECO:0000250"
FT DISULFID 164..191
FT /evidence="ECO:0000250"
FT DISULFID 367..398
FT /evidence="ECO:0000250"
FT VAR_SEQ 328..359
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_027537"
SQ SEQUENCE 433 AA; 48580 MW; FC43FB55026CD23B CRC64;
MSQLRTTKAG LVACGMICAF IFLYLRNPGP EEAEAEAEAE PTNPAVVECG FYPDELCSAL
FDGKKAAPQI AQFCKPPQNS EVPARLRTPG NCSRLARGLH FITRPLSAEE GNFSLAYVIH
APRELVMFVR LLRAIYAPQN VYCIHSDENA PKKFKSAMQT FVDCFGNIFL SSKTQKVAHD
NLRRLQAEID CMRDLVHSPF QWHYVMNLCG QEFPIKTNKE IIYDIRTRWK GKNITPGVTP
PANSKPKTGQ GPPKPSPDEN SYTAPNTIFK QSPPHNLTIS SGSAHYALTR KFVEFVLTDP
RAKDMLQWSK DIQSPEKHYW VTLNRLKDAP GATPDAGWEG HIRATKWRTE AGDGRKGCTD
HDAQDTCVYG LGDLPGLIRL PAFFATLEPS SDPLVGVCLE RRHRLRALQQ AEVPPEPHWQ
FPQQSHFNSQ PHH
