GCP2_MOUSE
ID GCP2_MOUSE Reviewed; 905 AA.
AC Q921G8; G5E859;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2012, sequence version 2.
DT 03-AUG-2022, entry version 132.
DE RecName: Full=Gamma-tubulin complex component 2;
DE Short=GCP-2;
GN Name=Tubgcp2; Synonyms=Gcp2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Kidney, Lung, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Gamma-tubulin complex is necessary for microtubule nucleation
CC at the centrosome. Plays a role in neuronal migration.
CC {ECO:0000250|UniProtKB:Q9BSJ2}.
CC -!- SUBUNIT: Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2,
CC TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. Interacts with ATF5; the
CC ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite unites the mother
CC centriole and the pericentriolar material (PCM) in the centrosome.
CC {ECO:0000250|UniProtKB:Q9BSJ2}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250|UniProtKB:Q9BSJ2}.
CC -!- SIMILARITY: Belongs to the TUBGCP family. {ECO:0000305}.
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DR EMBL; AC107822; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC109205; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH466531; EDL17885.1; -; Genomic_DNA.
DR EMBL; BC012519; AAH12519.1; -; mRNA.
DR EMBL; BC025582; AAH25582.1; -; mRNA.
DR CCDS; CCDS21960.1; -.
DR RefSeq; NP_001272936.1; NM_001286007.1.
DR RefSeq; NP_598516.2; NM_133755.2.
DR AlphaFoldDB; Q921G8; -.
DR SMR; Q921G8; -.
DR BioGRID; 216597; 29.
DR CORUM; Q921G8; -.
DR IntAct; Q921G8; 6.
DR MINT; Q921G8; -.
DR STRING; 10090.ENSMUSP00000026547; -.
DR iPTMnet; Q921G8; -.
DR PhosphoSitePlus; Q921G8; -.
DR EPD; Q921G8; -.
DR MaxQB; Q921G8; -.
DR PaxDb; Q921G8; -.
DR PRIDE; Q921G8; -.
DR ProteomicsDB; 271195; -.
DR Antibodypedia; 46457; 176 antibodies from 29 providers.
DR DNASU; 74237; -.
DR Ensembl; ENSMUST00000026547; ENSMUSP00000026547; ENSMUSG00000025474.
DR GeneID; 74237; -.
DR KEGG; mmu:74237; -.
DR UCSC; uc009kgl.2; mouse.
DR CTD; 10844; -.
DR MGI; MGI:1921487; Tubgcp2.
DR VEuPathDB; HostDB:ENSMUSG00000025474; -.
DR eggNOG; KOG2001; Eukaryota.
DR GeneTree; ENSGT00940000156697; -.
DR HOGENOM; CLU_007738_1_0_1; -.
DR InParanoid; Q921G8; -.
DR OMA; SKLMTCC; -.
DR OrthoDB; 679187at2759; -.
DR PhylomeDB; Q921G8; -.
DR TreeFam; TF324047; -.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR BioGRID-ORCS; 74237; 20 hits in 72 CRISPR screens.
DR ChiTaRS; Tubgcp2; mouse.
DR PRO; PR:Q921G8; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q921G8; protein.
DR Bgee; ENSMUSG00000025474; Expressed in spermatocyte and 251 other tissues.
DR ExpressionAtlas; Q921G8; baseline and differential.
DR Genevisible; Q921G8; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; IEA:UniProt.
DR GO; GO:0000923; C:equatorial microtubule organizing center; IBA:GO_Central.
DR GO; GO:0000930; C:gamma-tubulin complex; IBA:GO_Central.
DR GO; GO:0008275; C:gamma-tubulin small complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005815; C:microtubule organizing center; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0000922; C:spindle pole; IEA:InterPro.
DR GO; GO:0043015; F:gamma-tubulin binding; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; ISS:UniProtKB.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; IBA:GO_Central.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0001764; P:neuron migration; ISS:UniProtKB.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR Gene3D; 1.20.120.1900; -; 1.
DR InterPro; IPR007259; GCP.
DR InterPro; IPR040457; GCP_C.
DR InterPro; IPR042241; GCP_C_sf.
DR InterPro; IPR041470; GCP_N.
DR PANTHER; PTHR19302; PTHR19302; 1.
DR Pfam; PF04130; GCP_C_terminal; 1.
DR Pfam; PF17681; GCP_N_terminal; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Microtubule; Phosphoprotein; Reference proteome.
FT CHAIN 1..905
FT /note="Gamma-tubulin complex component 2"
FT /id="PRO_0000078114"
FT REGION 877..905
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 83
FT /note="Phosphotyrosine"
FT /evidence="ECO:0000250|UniProtKB:Q9BSJ2"
FT CONFLICT 727
FT /note="T -> I (in Ref. 3; AAH12519/AAH25582)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 905 AA; 103223 MW; D5FEFF23AC4AB52E CRC64;
MSEFRIHHDV NELLSLLRIH GGDGAEVYID LLQKNRTPYV TTTVSAHSAK VKIAEFSRTP
EDFLKKYDEL KSKNTRNLDP LVYLLSKLTE DKETLQFLQQ NAKERAELAA SAATSNTTSF
SIPVAASKMS TQELEELRKQ LGSVSTGSTL QQSLELTRKM LRDKQNKKNS GQPLPVFPAW
VYERPTLAGD FLIGSGLSSD TVLPIGTLPL ASQESAVVED LLYVLVGVDG RYITAQPLAG
RQNRTFLVDP NLDLSIRELV NRILPVAASY STVTRFIEEK SSFEYGQVNH ALAAAMRTLV
KEYLILVTQL EQLHRQGLLS LQKLWFYIQP AMRTIDILAS LATSVDKGEC VGGSTLSLLH
DRSFNYTGDS QAQELCLYLT KAASAPYFEI LEKWIYRGII HDPYSEFMVE EHELRKEKIQ
EDYNDKYWDQ RYTVLPQQIP SFLQKVAGKI LSTGKYLNVV RECGHDVTCP VAKEIIYTLK
ERAYVEQIEK AFNYASKVLL DFLMEEKELV AHLRSIKRYF LMDQGDFFVH FMDLTEEELR
KPVEDIILTR LEALLELALR MSTANTDPFK DDLKIELMPH DLITQLLRVL AIETKQEKAM
THADPTELTL SGLEAFSFDY MVKWPLSLII NRKALTRYQM LFRHMFYCKH VERQLCSVWI
SNKTAKQHAL HSAKWFAGAF TLRQRMLNFV QNIQYYMMFE VMEPTWHILE KNLKSASNID
DVLGHHTSFL DNCLKDCMLT NPELLKVFSK LMSVCVMFTN CMQKFTQSMK LDSELGRLTL
DQGSVQGPPT ETERIEDRPR KELTRKHLSE HVDAPQLASG FEATINNFDK NFSAHLLDLL
ARLSVYSTSD CEHGMASVIS RLDFNGFYAE RLERLSAERS QKAAPQVPVP RGPSAPAPRV
AIPAQ
