GCP3_DROME
ID GCP3_DROME Reviewed; 917 AA.
AC Q9XYP8; Q9VY94;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 06-JUN-2002, sequence version 2.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Gamma-tubulin complex component 3 {ECO:0000312|FlyBase:FBgn0001612};
DE AltName: Full=Gamma-ring complex protein 91 kDa {ECO:0000303|PubMed:10037793};
GN Name=Grip91 {ECO:0000303|PubMed:10037793, ECO:0000312|FlyBase:FBgn0001612};
GN Synonyms=l(1)dd4 {ECO:0000303|PubMed:10037793};
GN ORFNames=CG10988 {ECO:0000312|FlyBase:FBgn0001612};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PROTEIN SEQUENCE OF 24-36; 85-90; 202-227;
RP 250-257; 277-302; 541-548; 616-626; 664-671; 799-804; 810-817 AND 863-880.
RX PubMed=10037793; DOI=10.1083/jcb.144.4.721;
RA Oegema K., Wiese C., Martin O.C., Milligan R.A., Iwamatsu A.,
RA Mitchison T.J., Zheng Y.;
RT "Characterization of two related Drosophila gamma-tubulin complexes that
RT differ in their ability to nucleate microtubules.";
RL J. Cell Biol. 144:721-733(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Ovary;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- SUBUNIT: Gamma-tubulin small complex (Gamma TuSC) is a heterotetrameric
CC complex which contains two molecules of gamma-tubulin, and one molecule
CC each of Dgrip84 and Dgrip91. The gamma-tubulin in this complex binds
CC preferentially to GDP over GTP.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TUBGCP family. {ECO:0000305}.
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DR EMBL; AF118380; AAD27817.1; -; mRNA.
DR EMBL; AE014298; AAF48309.1; -; Genomic_DNA.
DR EMBL; AY069292; AAL39437.1; -; mRNA.
DR RefSeq; NP_524919.2; NM_080180.4.
DR AlphaFoldDB; Q9XYP8; -.
DR SMR; Q9XYP8; -.
DR BioGRID; 71426; 14.
DR DIP; DIP-19100N; -.
DR IntAct; Q9XYP8; 5.
DR MINT; Q9XYP8; -.
DR STRING; 7227.FBpp0073672; -.
DR PaxDb; Q9XYP8; -.
DR PRIDE; Q9XYP8; -.
DR EnsemblMetazoa; FBtr0073841; FBpp0073672; FBgn0001612.
DR GeneID; 48481; -.
DR KEGG; dme:Dmel_CG10988; -.
DR CTD; 48481; -.
DR FlyBase; FBgn0001612; Grip91.
DR VEuPathDB; VectorBase:FBgn0001612; -.
DR eggNOG; KOG2000; Eukaryota.
DR GeneTree; ENSGT00940000157872; -.
DR HOGENOM; CLU_003736_3_0_1; -.
DR InParanoid; Q9XYP8; -.
DR OMA; MRMMSVC; -.
DR OrthoDB; 480444at2759; -.
DR PhylomeDB; Q9XYP8; -.
DR SignaLink; Q9XYP8; -.
DR BioGRID-ORCS; 48481; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 48481; -.
DR PRO; PR:Q9XYP8; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0001612; Expressed in cleaving embryo and 23 other tissues.
DR ExpressionAtlas; Q9XYP8; baseline and differential.
DR Genevisible; Q9XYP8; DM.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000923; C:equatorial microtubule organizing center; IBA:GO_Central.
DR GO; GO:0000930; C:gamma-tubulin complex; IBA:GO_Central.
DR GO; GO:0008275; C:gamma-tubulin small complex; IDA:UniProtKB.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0000922; C:spindle pole; IEA:InterPro.
DR GO; GO:0043015; F:gamma-tubulin binding; IDA:FlyBase.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0007140; P:male meiotic nuclear division; IMP:FlyBase.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0000212; P:meiotic spindle organization; IMP:FlyBase.
DR GO; GO:0007020; P:microtubule nucleation; IDA:UniProtKB.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR Gene3D; 1.20.120.1900; -; 1.
DR InterPro; IPR007259; GCP.
DR InterPro; IPR040457; GCP_C.
DR InterPro; IPR042241; GCP_C_sf.
DR InterPro; IPR041470; GCP_N.
DR PANTHER; PTHR19302; PTHR19302; 1.
DR Pfam; PF04130; GCP_C_terminal; 1.
DR Pfam; PF17681; GCP_N_terminal; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Direct protein sequencing; Microtubule;
KW Reference proteome.
FT CHAIN 1..917
FT /note="Gamma-tubulin complex component 3"
FT /id="PRO_0000078121"
FT CONFLICT 79
FT /note="N -> Y (in Ref. 1; AAD27817)"
FT /evidence="ECO:0000305"
FT CONFLICT 118
FT /note="M -> I (in Ref. 1; AAD27817)"
FT /evidence="ECO:0000305"
FT CONFLICT 413
FT /note="L -> F (in Ref. 1; AAD27817)"
FT /evidence="ECO:0000305"
FT CONFLICT 479
FT /note="L -> F (in Ref. 1; AAD27817)"
FT /evidence="ECO:0000305"
FT CONFLICT 583
FT /note="G -> A (in Ref. 1; AAD27817)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 917 AA; 103707 MW; 6AEE88C211D256BB CRC64;
MSQDRIAGID VATNSTDISN IINEMIICIK GKQMPEVHEK AMDHLSKMIA ANSRVIRDSN
MLTERECVQK IMKLLSARNK KEEGKTVSDH FNELYRKLTL TKCDPHMRHS LMTHLLTMTD
NSDAEKAVAS EDPRTQCDNL TQILVSRLNS ISSSIASLNE MGVVNGNGVG AAAVTGAAAV
TGAAAVTGAA AVTGAAASHS YDATQSSIGL RKQSLPNYLD ATKMLPESRH DIVMSAIYSF
TGVQGKYLKK DVVTGRFKLD QQNIKFLTTG QAGMLLRLSE LGYYHDRVVK FSDVSTGFNA
IGSMGQALIS KLKEELANFH GQVAMLHDEM QRFRQASVNG IANKGKKDSG PDAGDEMTLF
KLLAWYIKPL HRMQWLTKIA DACQVKKGGD LASTVYDFLD NGNDMVNKLV EDLLTAICGP
LVRMISKWIL EGGISDMHRE FFVKSIKDVG VDRLWHDKFR LRLPMLPKFV PMDMANKILM
TGKSINFLRE ICEEQGMMKE RDELMKVMES SASQIFSYTP DTSWHAAVET CYQQTSKHVL
DIMVGPHKLL DHLHGMRRYL LLGQGDFISI LIENMKNELE RPGLDIYAND LTSMLDSALR
CTNAQYDDPD ILNHLDVIVQ RPFNGDIGWN IISLQYIVHG PLAAMLESTM PTYKVLFKPL
WRMKHMEFVL SMKIWKEQMG NAKALRTMKS EIGKASHRLN LFTSEIMHFI HQMQYYVLFE
VIECNWVELQ KKMQKATTLD EILEAHEKFL QTILVGCFVS NKASVEHSLE VVYENIIELE
KWQSSFYKDC FKELNARKEL SKIVEKSEKK GVYGLTNKMI LQRDQEAKIF AEKMDIACRG
LEVIATDYEK AVSTFLMSLN SSDDPNLQLF GTRLDFNEYY KKRDTNLSKP LTFEHMRMSN
VFAVNSRFVI CTPSTQE
