GCP3_MOUSE
ID GCP3_MOUSE Reviewed; 905 AA.
AC P58854; Q6PDQ9;
DT 06-JUN-2002, integrated into UniProtKB/Swiss-Prot.
DT 11-OCT-2005, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Gamma-tubulin complex component 3;
DE Short=GCP-3;
GN Name=Tubgcp3; Synonyms=Gcp3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Bone marrow;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Eye, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP INTERACTION WITH NIN.
RX PubMed=15784680; DOI=10.1242/jcs.02302;
RA Delgehyr N., Sillibourne J., Bornens M.;
RT "Microtubule nucleation and anchoring at the centrosome are independent
RT processes linked by ninein function.";
RL J. Cell Sci. 118:1565-1575(2005).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Gamma-tubulin complex is necessary for microtubule nucleation
CC at the centrosome. {ECO:0000250}.
CC -!- SUBUNIT: Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2,
CC TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6 (By similarity). Interacts with
CC CDK5RAP2; the interaction is leading to centrosomal localization of
CC TUBTUBGCP3 and CDK5RAP2 (By similarity). Interacts with NIN (via N-
CC terminus); the interaction may promote recruitment of the gamma-tubulin
CC ring complex to the centrosome (PubMed:15784680). {ECO:0000250,
CC ECO:0000269|PubMed:15784680}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TUBGCP family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AK150149; BAE29343.1; -; mRNA.
DR EMBL; BC025647; AAH25647.1; -; mRNA.
DR EMBL; BC058566; AAH58566.1; -; mRNA.
DR CCDS; CCDS22103.1; -.
DR RefSeq; NP_932148.1; NM_198031.1.
DR AlphaFoldDB; P58854; -.
DR SMR; P58854; -.
DR BioGRID; 234417; 26.
DR CORUM; P58854; -.
DR IntAct; P58854; 13.
DR MINT; P58854; -.
DR STRING; 10090.ENSMUSP00000000776; -.
DR iPTMnet; P58854; -.
DR PhosphoSitePlus; P58854; -.
DR EPD; P58854; -.
DR MaxQB; P58854; -.
DR PaxDb; P58854; -.
DR PeptideAtlas; P58854; -.
DR PRIDE; P58854; -.
DR ProteomicsDB; 268860; -.
DR Antibodypedia; 25680; 218 antibodies from 29 providers.
DR DNASU; 259279; -.
DR Ensembl; ENSMUST00000000776; ENSMUSP00000000776; ENSMUSG00000000759.
DR GeneID; 259279; -.
DR KEGG; mmu:259279; -.
DR UCSC; uc009kwd.1; mouse.
DR CTD; 10426; -.
DR MGI; MGI:2183752; Tubgcp3.
DR VEuPathDB; HostDB:ENSMUSG00000000759; -.
DR eggNOG; KOG2000; Eukaryota.
DR GeneTree; ENSGT00940000157872; -.
DR HOGENOM; CLU_003736_5_0_1; -.
DR InParanoid; P58854; -.
DR OMA; MRMMSVC; -.
DR OrthoDB; 480444at2759; -.
DR PhylomeDB; P58854; -.
DR TreeFam; TF300705; -.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR BioGRID-ORCS; 259279; 19 hits in 74 CRISPR screens.
DR ChiTaRS; Tubgcp3; mouse.
DR PRO; PR:P58854; -.
DR Proteomes; UP000000589; Chromosome 8.
DR RNAct; P58854; protein.
DR Bgee; ENSMUSG00000000759; Expressed in ileal epithelium and 248 other tissues.
DR ExpressionAtlas; P58854; baseline and differential.
DR Genevisible; P58854; MM.
DR GO; GO:0005814; C:centriole; ISO:MGI.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005829; C:cytosol; IEA:UniProt.
DR GO; GO:0000923; C:equatorial microtubule organizing center; IBA:GO_Central.
DR GO; GO:0000930; C:gamma-tubulin complex; IBA:GO_Central.
DR GO; GO:0008275; C:gamma-tubulin small complex; IBA:GO_Central.
DR GO; GO:0005827; C:polar microtubule; ISO:MGI.
DR GO; GO:0043015; F:gamma-tubulin binding; ISO:MGI.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; IBA:GO_Central.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR Gene3D; 1.20.120.1900; -; 1.
DR InterPro; IPR007259; GCP.
DR InterPro; IPR040457; GCP_C.
DR InterPro; IPR042241; GCP_C_sf.
DR InterPro; IPR041470; GCP_N.
DR PANTHER; PTHR19302; PTHR19302; 1.
DR Pfam; PF04130; GCP_C_terminal; 1.
DR Pfam; PF17681; GCP_N_terminal; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cytoplasm; Cytoskeleton; Microtubule; Phosphoprotein;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:Q96CW5"
FT CHAIN 2..905
FT /note="Gamma-tubulin complex component 3"
FT /id="PRO_0000078119"
FT REGION 174..193
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 206..239
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 206..228
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 2
FT /note="N-acetylalanine"
FT /evidence="ECO:0000250|UniProtKB:Q96CW5"
FT MOD_RES 113
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q96CW5"
FT CONFLICT 229..232
FT /note="TRSR -> HASG (in Ref. 2; AAH25647)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 905 AA; 103469 MW; 7140808D349A471E CRC64;
MATPDQKSPN VLLQNLCCRI LGRSEADVAQ QFQFAVRVIG SNFAPTVERD EFLVAEKIKK
ELIRQRREAD AALFSELHRK LHSQGVLKNK WSILYLLLNL SEDPRKQASK VTSYASLFAQ
ALPRDAHSTP YYYARPQTLP LNYQDRSTQA QSSGSLGSSG ISSIGMCGLS GPTPVQPFLP
GQSHQAPGVG DGLRQQLGPR LAWTLTGNQP SSQTPTSKGF PNALSRNLTR SRREGDPGGT
LEVTEAALVR DILYVFQGID GKNIKMSSTE NCYKVEAKAN LNKSLRDTAV RLAELGWLHN
KIRKYADQRS LDRSFGLVGQ SFCAALHQEL KEYYRLLSVL HSQLQLEDDQ GVNLGLESSL
TLRRLLVWTY DPKIRLKTLA ALVDHCQGRK GGELASAVHA YTKTGDPYMK SLVQHILSLV
SHPVLSFLYR WIYDGELEDT YHEFFVASDP TVKTDRLWHD KYTLRKSMIP SFITMDQSRK
VLLIGKSINF LHQVCHDQTP TTKMIAVTKS AESPRDAADL FTDLENAFQG KIDAAYFETS
KYLLDVLNKK YSLLEHMQAM RRYLLLGQGD FIRHLMDLLK PELVRPATTL YQHNLTGILE
TAVRATNAQF DSPEILKRLD VRLLEVSPGD TGWDVFSLDY HVDGPIATVF TRECMSHYLR
VFNFLWRAKR MEYILTDIRK GHMCNAKLLR NMPEFSGVLH QCHILASEMV HFIHQMQYYI
TFEVLECSWD ELWNRVQQAQ DLDHIIAAHE AFLNTITSRC LLDSNSRVLL NQLRAVFDQI
IELQNAQDVM YRAALEELQR RLQFEEKKKQ REIEGQWGVT AAEEEEENKR IREFQDSIPK
MCSQLRILTH FYQGVVQQFL VLLTTSSDES LQFLSFRLDF NEHYKAREPR LRVSLGSRGR
RSSHT
