GCP4_DROME
ID GCP4_DROME Reviewed; 650 AA.
AC Q9VKU7; Q9U1J7;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2002, sequence version 2.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Gamma-tubulin complex component 4 homolog;
DE AltName: Full=Gamma-ring complex protein 75 kDa;
DE Short=d75p;
DE Short=dGrip75;
GN Name=Grip75; Synonyms=75p; ORFNames=CG6176;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10562286; DOI=10.1083/jcb.147.4.857;
RA Fava F., Raynaud-Messina B., Leung-Tack J., Mazzolini L., Li M.,
RA Guillemot J.-C., Cachot D., Tollon Y., Ferrara P., Wright M.;
RT "Human 76p: a new protein member of the gamma-tubulin associated protein
RT family.";
RL J. Cell Biol. 147:857-868(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-214; THR-216 AND SER-218, AND
RP IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Gamma-tubulin complex is necessary for microtubule nucleation
CC at the centrosome. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the TUBGCP family. {ECO:0000305}.
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DR EMBL; AE014134; AAF52962.2; -; Genomic_DNA.
DR EMBL; AJ249678; CAB62508.1; -; mRNA.
DR RefSeq; NP_609412.1; NM_135568.4.
DR AlphaFoldDB; Q9VKU7; -.
DR SMR; Q9VKU7; -.
DR BioGRID; 60521; 8.
DR IntAct; Q9VKU7; 4.
DR MINT; Q9VKU7; -.
DR STRING; 7227.FBpp0079623; -.
DR iPTMnet; Q9VKU7; -.
DR PaxDb; Q9VKU7; -.
DR PRIDE; Q9VKU7; -.
DR DNASU; 34441; -.
DR EnsemblMetazoa; FBtr0080033; FBpp0079623; FBgn0026431.
DR GeneID; 34441; -.
DR KEGG; dme:Dmel_CG6176; -.
DR UCSC; CG6176-RA; d. melanogaster.
DR CTD; 34441; -.
DR FlyBase; FBgn0026431; Grip75.
DR VEuPathDB; VectorBase:FBgn0026431; -.
DR eggNOG; KOG2065; Eukaryota.
DR HOGENOM; CLU_012029_0_0_1; -.
DR InParanoid; Q9VKU7; -.
DR OMA; WAAVMHQ; -.
DR OrthoDB; 1137416at2759; -.
DR PhylomeDB; Q9VKU7; -.
DR BioGRID-ORCS; 34441; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 34441; -.
DR PRO; PR:Q9VKU7; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0026431; Expressed in eye disc (Drosophila) and 28 other tissues.
DR ExpressionAtlas; Q9VKU7; baseline and differential.
DR Genevisible; Q9VKU7; DM.
DR GO; GO:0005813; C:centrosome; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0000923; C:equatorial microtubule organizing center; IBA:GO_Central.
DR GO; GO:0000930; C:gamma-tubulin complex; IBA:GO_Central.
DR GO; GO:0000931; C:gamma-tubulin large complex; NAS:FlyBase.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0000922; C:spindle pole; IEA:InterPro.
DR GO; GO:0043015; F:gamma-tubulin binding; IBA:GO_Central.
DR GO; GO:0005200; F:structural constituent of cytoskeleton; NAS:FlyBase.
DR GO; GO:0045450; P:bicoid mRNA localization; IMP:FlyBase.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0007112; P:male meiosis cytokinesis; IMP:FlyBase.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; IBA:GO_Central.
DR GO; GO:0000278; P:mitotic cell cycle; IMP:FlyBase.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR GO; GO:0007058; P:spindle assembly involved in female meiosis II; IMP:FlyBase.
DR Gene3D; 1.20.120.1900; -; 1.
DR InterPro; IPR007259; GCP.
DR InterPro; IPR040457; GCP_C.
DR InterPro; IPR042241; GCP_C_sf.
DR InterPro; IPR041470; GCP_N.
DR PANTHER; PTHR19302; PTHR19302; 1.
DR Pfam; PF04130; GCP_C_terminal; 1.
DR Pfam; PF17681; GCP_N_terminal; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Microtubule; Phosphoprotein; Reference proteome.
FT CHAIN 1..650
FT /note="Gamma-tubulin complex component 4 homolog"
FT /id="PRO_0000078126"
FT MOD_RES 214
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 216
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT MOD_RES 218
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:18327897"
SQ SEQUENCE 650 AA; 74980 MW; 515D8B36A80D08BE CRC64;
MIHDLLLACR SHNPEQLGIK AFNETTVIDQ FIHPCEREIF MDIIKIIKVY QEVEQFTHSS
GRKSDTHGEL PDSLHGYYLL NLAKGIEMAL EEYYAEIGRL EKYCLGNERN SLSYVYNALY
AKFPLLVFMR NLITEIHVLN LRGCVLLHNL HQQCEHGDIQ LEKAIKIIMK PVKNAFFSSL
AHWLLFGVID DVHSEFFIKF TPTDAVDGSS FSKSATCSLL SAEKNPEDYI WQYEVNMSQL
PGFFSIVLAE KVLFVGQTVL VFKMGRNVKV KNKTDPLAAK LAELDSDDIY QLWSGRESEF
FKMVVDLSNE DTINVFRLEK VIIDIKNYVS ARLSEIAVNE VDLERQMGLI KDFFLLGRGE
FYLEFCSQMV GTMETYREER FKNVTRSFEL AATVTGITDD LDKFSLICQR STSEPDDTSD
FNFLQGLSLK YEYEWPLNLL FSPTTIERYN NIFRFLLIIR TYQYEIQRVW AKQTWRAKSA
KDVPPNNKII TLRNYLMFFL NNMQYYIQVD VLESQFGILM NVIKSRSDFE VIQRAHTVFL
ANVLSHCFLL NESETQLNVT GSQNRNPIYG TLLKLFGICE KFAHMTQTKD PSDDLEDEVD
QLNESFGVQI ASLIQLLVDV KSASCLGPLS QLLLRLDFNC WFSASHNTSA
