GCP4_MOUSE
ID GCP4_MOUSE Reviewed; 667 AA.
AC Q9D4F8;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 02-AUG-2002, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Gamma-tubulin complex component 4;
DE Short=GCP-4;
GN Name=Tubgcp4; Synonyms=D2Ertd435e, Gcp4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 26-338.
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Gamma-tubulin complex is necessary for microtubule nucleation
CC at the centrosome. {ECO:0000250|UniProtKB:Q9UGJ1}.
CC -!- SUBUNIT: Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2,
CC TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. Interacts with NINL. Interacts
CC with ATF5; the ATF5:PCNT:polyglutamylated tubulin (PGT) tripartite
CC unites the mother centriole and the pericentriolar material (PCM) in
CC the centrosome. {ECO:0000250|UniProtKB:Q9UGJ1}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250|UniProtKB:Q9UGJ1}.
CC -!- SIMILARITY: Belongs to the TUBGCP family. {ECO:0000305}.
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DR EMBL; BC029106; AAH29106.1; -; mRNA.
DR EMBL; AK016563; -; NOT_ANNOTATED_CDS; mRNA.
DR CCDS; CCDS16637.1; -.
DR RefSeq; NP_700436.1; NM_153387.3.
DR AlphaFoldDB; Q9D4F8; -.
DR SMR; Q9D4F8; -.
DR BioGRID; 206244; 29.
DR IntAct; Q9D4F8; 9.
DR MINT; Q9D4F8; -.
DR STRING; 10090.ENSMUSP00000044049; -.
DR PhosphoSitePlus; Q9D4F8; -.
DR EPD; Q9D4F8; -.
DR MaxQB; Q9D4F8; -.
DR PaxDb; Q9D4F8; -.
DR PRIDE; Q9D4F8; -.
DR ProteomicsDB; 271196; -.
DR Antibodypedia; 23879; 226 antibodies from 28 providers.
DR DNASU; 51885; -.
DR Ensembl; ENSMUST00000039541; ENSMUSP00000044049; ENSMUSG00000027263.
DR GeneID; 51885; -.
DR KEGG; mmu:51885; -.
DR UCSC; uc008lxv.2; mouse.
DR CTD; 27229; -.
DR MGI; MGI:1196293; Tubgcp4.
DR VEuPathDB; HostDB:ENSMUSG00000027263; -.
DR eggNOG; KOG2065; Eukaryota.
DR GeneTree; ENSGT00940000156677; -.
DR InParanoid; Q9D4F8; -.
DR OMA; WAAVMHQ; -.
DR OrthoDB; 1137416at2759; -.
DR PhylomeDB; Q9D4F8; -.
DR TreeFam; TF324188; -.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR BioGRID-ORCS; 51885; 20 hits in 73 CRISPR screens.
DR ChiTaRS; Tubgcp4; mouse.
DR PRO; PR:Q9D4F8; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9D4F8; protein.
DR Bgee; ENSMUSG00000027263; Expressed in floor plate of midbrain and 263 other tissues.
DR ExpressionAtlas; Q9D4F8; baseline and differential.
DR Genevisible; Q9D4F8; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; IEA:UniProt.
DR GO; GO:0000923; C:equatorial microtubule organizing center; IBA:GO_Central.
DR GO; GO:0000930; C:gamma-tubulin complex; IBA:GO_Central.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0055037; C:recycling endosome; ISO:MGI.
DR GO; GO:0000922; C:spindle pole; IEA:InterPro.
DR GO; GO:0043015; F:gamma-tubulin binding; IBA:GO_Central.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; IBA:GO_Central.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR Gene3D; 1.20.120.1900; -; 1.
DR InterPro; IPR007259; GCP.
DR InterPro; IPR040457; GCP_C.
DR InterPro; IPR042241; GCP_C_sf.
DR InterPro; IPR041470; GCP_N.
DR PANTHER; PTHR19302; PTHR19302; 1.
DR Pfam; PF04130; GCP_C_terminal; 1.
DR Pfam; PF17681; GCP_N_terminal; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Cytoskeleton; Microtubule; Reference proteome.
FT CHAIN 1..667
FT /note="Gamma-tubulin complex component 4"
FT /id="PRO_0000078125"
FT REGION 424..446
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 424..440
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 667 AA; 76126 MW; 32CC26F5D69D2CF6 CRC64;
MIHELLLALS GYPGSIFTWN KRSGLQVSQD FPFLHPSETS VLNRLCRLGT DYIRFTEFIE
QYTGHVQQQD HHPPQQGQGG LHGIYLRAFC TGLDSVLQPY RQALLDLEQE FLADPHLSIS
HVNYSLDQFQ LLFPSVMVVV EQIKSQKIHG CQILETVYKH SCGGLPPVRS ALEKILAVCH
GVMYKQLSAW MLHGLLLDQH EEFFIKQGPS SGTLSAQLEE DEEDLGIGGL TGKQLRELQD
LRLIEEENML APSLKQFSLR VEILPSYIPV RVAEKILFVG ESVQMFENQN VNLTRKGSIL
KNQEDTFAAE LHRLKQQPLF SLVDFEQVVD RIRSTVAEHL WKLMVEESDL LGQLKIIKDF
YLLGRGELFQ AFIDTAQHML KTPPTAVTEH DVNVAFQQSA HKVLLDDDNL LPLLHLTIEY
HGKDHKADAT QPREVPSRET SPREAPSSGW AALGLSYKVQ WPLHILFTPA VLEKYNVVFK
YLLSVRRVQA ELQHCWALQM QRKHLKSNQT DAVKWRLRNH MAFLVDNLQY YLQVDVLESQ
FSQLLHQINS TRDFESIRLA HDHFLSNLLA QSFILLKPVF HCLNEILDLC HSFCSLVSQN
LGPLDERGAA QLSILVKGFS RQSSLLFKIL SSVRNHQINS DLAQLLLRLD YNKYYTQAGG
TLGSFGM
