GCP5_HUMAN
ID GCP5_HUMAN Reviewed; 1024 AA.
AC Q96RT8; E9PB12; Q6IQ52; Q96PY8;
DT 02-MAY-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 172.
DE RecName: Full=Gamma-tubulin complex component 5;
DE Short=GCP-5;
GN Name=TUBGCP5; Synonyms=GCP5, KIAA1899;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), AND CHARACTERIZATION.
RX PubMed=11694571; DOI=10.1091/mbc.12.11.3340;
RA Murphy S.M., Preble A.M., Patel U.K., O'Connell K.L., Dias D.P., Moritz M.,
RA Agard D., Stults J.T., Stearns T.;
RT "GCP5 and GCP6: two new members of the human gamma-tubulin complex.";
RL Mol. Biol. Cell 12:3340-3352(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain;
RX PubMed=11572484; DOI=10.1093/dnares/8.4.179;
RA Nagase T., Kikuno R., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XXI. The
RT complete sequences of 60 new cDNA clones from brain which code for large
RT proteins.";
RL DNA Res. 8:179-187(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [5]
RP SEQUENCE REVISION.
RA Ohara O., Nagase T., Kikuno R.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Brain, and Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=14508708; DOI=10.1086/378816;
RA Chai J.-H., Locke D.P., Greally J.M., Knoll J.H.M., Ohta T., Dunai J.,
RA Yavor A., Eichler E.E., Nicholls R.D.;
RT "Identification of four highly conserved genes between breakpoint hotspots
RT BP1 and BP2 of the Prader-Willi/Angelman syndromes deletion region that
RT have undergone evolutionary transposition mediated by flanking duplicons.";
RL Am. J. Hum. Genet. 73:898-925(2003).
RN [8]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RC TISSUE=Lymphoblast;
RX PubMed=14654843; DOI=10.1038/nature02166;
RA Andersen J.S., Wilkinson C.J., Mayor T., Mortensen P., Nigg E.A., Mann M.;
RT "Proteomic characterization of the human centrosome by protein correlation
RT profiling.";
RL Nature 426:570-574(2003).
RN [9]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Gamma-tubulin complex is necessary for microtubule nucleation
CC at the centrosome.
CC -!- SUBUNIT: Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2,
CC TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6.
CC -!- INTERACTION:
CC Q96RT8; Q9Y5R4: HEMK1; NbExp=3; IntAct=EBI-2555061, EBI-10329202;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000269|PubMed:14654843}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96RT8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96RT8-2; Sequence=VSP_047496;
CC -!- TISSUE SPECIFICITY: Widely expressed, with highest levels in heart and
CC skeletal muscle and moderate levels in brain.
CC {ECO:0000269|PubMed:14508708}.
CC -!- SIMILARITY: Belongs to the TUBGCP family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH46182.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AK056416; Type=Erroneous termination; Note=Truncated C-terminus.; Evidence={ECO:0000305};
CC Sequence=AK056416; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=BAB67792.2; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF272884; AAK77662.1; -; mRNA.
DR EMBL; AB067486; BAB67792.2; ALT_INIT; mRNA.
DR EMBL; AK056416; -; NOT_ANNOTATED_CDS; mRNA.
DR EMBL; AC116166; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC046182; AAH46182.1; ALT_INIT; mRNA.
DR EMBL; BC071560; AAH71560.1; -; mRNA.
DR CCDS; CCDS73697.1; -. [Q96RT8-2]
DR CCDS; CCDS73698.1; -. [Q96RT8-1]
DR RefSeq; NP_001096080.1; NM_001102610.1. [Q96RT8-2]
DR RefSeq; NP_443135.3; NM_052903.4. [Q96RT8-1]
DR PDB; 6L81; X-ray; 2.20 A; A/C=1-119.
DR PDB; 6V69; EM; 4.20 A; J=1-1024.
DR PDB; 6V6S; EM; 4.30 A; J=1-1024.
DR PDB; 7AS4; EM; 4.13 A; J=1-1024.
DR PDB; 7QJ0; EM; 5.32 A; J/l=1-1024.
DR PDB; 7QJ1; EM; 7.00 A; J/l=1-1024.
DR PDB; 7QJ2; EM; 8.60 A; J/l=1-1024.
DR PDB; 7QJ3; EM; 7.60 A; J/l=1-1024.
DR PDB; 7QJ4; EM; 9.00 A; J/l=1-1024.
DR PDB; 7QJ5; EM; 8.70 A; J/l=1-1024.
DR PDB; 7QJ6; EM; 7.80 A; J/l=1-1024.
DR PDB; 7QJ7; EM; 8.70 A; J/l=1-1024.
DR PDB; 7QJ8; EM; 8.70 A; J/l=1-1024.
DR PDB; 7QJ9; EM; 8.10 A; J/l=1-1024.
DR PDB; 7QJA; EM; 9.20 A; J/l=1-1024.
DR PDB; 7QJB; EM; 9.20 A; J/l=1-1024.
DR PDB; 7QJC; EM; 16.10 A; J/l=1-1024.
DR PDB; 7QJD; EM; 7.10 A; J/l=1-1024.
DR PDB; 7QJE; EM; 7.80 A; J=1-1024.
DR PDBsum; 6L81; -.
DR PDBsum; 6V69; -.
DR PDBsum; 6V6S; -.
DR PDBsum; 7AS4; -.
DR PDBsum; 7QJ0; -.
DR PDBsum; 7QJ1; -.
DR PDBsum; 7QJ2; -.
DR PDBsum; 7QJ3; -.
DR PDBsum; 7QJ4; -.
DR PDBsum; 7QJ5; -.
DR PDBsum; 7QJ6; -.
DR PDBsum; 7QJ7; -.
DR PDBsum; 7QJ8; -.
DR PDBsum; 7QJ9; -.
DR PDBsum; 7QJA; -.
DR PDBsum; 7QJB; -.
DR PDBsum; 7QJC; -.
DR PDBsum; 7QJD; -.
DR PDBsum; 7QJE; -.
DR AlphaFoldDB; Q96RT8; -.
DR SMR; Q96RT8; -.
DR BioGRID; 125353; 57.
DR CORUM; Q96RT8; -.
DR IntAct; Q96RT8; 39.
DR MINT; Q96RT8; -.
DR STRING; 9606.ENSP00000480316; -.
DR iPTMnet; Q96RT8; -.
DR PhosphoSitePlus; Q96RT8; -.
DR BioMuta; TUBGCP5; -.
DR DMDM; 20454926; -.
DR EPD; Q96RT8; -.
DR jPOST; Q96RT8; -.
DR MassIVE; Q96RT8; -.
DR MaxQB; Q96RT8; -.
DR PaxDb; Q96RT8; -.
DR PeptideAtlas; Q96RT8; -.
DR PRIDE; Q96RT8; -.
DR ProteomicsDB; 19119; -.
DR ProteomicsDB; 78032; -. [Q96RT8-1]
DR Antibodypedia; 72633; 148 antibodies from 27 providers.
DR DNASU; 114791; -.
DR Ensembl; ENST00000615383.5; ENSP00000480316.1; ENSG00000275835.5. [Q96RT8-1]
DR Ensembl; ENST00000620435.4; ENSP00000481853.1; ENSG00000275835.5. [Q96RT8-2]
DR GeneID; 114791; -.
DR KEGG; hsa:114791; -.
DR MANE-Select; ENST00000615383.5; ENSP00000480316.1; NM_052903.6; NP_443135.3.
DR UCSC; uc001yuq.3; human. [Q96RT8-1]
DR CTD; 114791; -.
DR DisGeNET; 114791; -.
DR GeneCards; TUBGCP5; -.
DR HGNC; HGNC:18600; TUBGCP5.
DR HPA; ENSG00000275835; Low tissue specificity.
DR MIM; 608147; gene.
DR neXtProt; NX_Q96RT8; -.
DR OpenTargets; ENSG00000275835; -.
DR PharmGKB; PA38599; -.
DR VEuPathDB; HostDB:ENSG00000275835; -.
DR eggNOG; KOG4344; Eukaryota.
DR GeneTree; ENSGT00940000155962; -.
DR HOGENOM; CLU_011574_0_0_1; -.
DR InParanoid; Q96RT8; -.
DR OMA; RTNQFEV; -.
DR OrthoDB; 110079at2759; -.
DR PhylomeDB; Q96RT8; -.
DR TreeFam; TF329759; -.
DR PathwayCommons; Q96RT8; -.
DR Reactome; R-HSA-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-HSA-380320; Recruitment of NuMA to mitotic centrosomes.
DR SignaLink; Q96RT8; -.
DR SIGNOR; Q96RT8; -.
DR BioGRID-ORCS; 114791; 694 hits in 1080 CRISPR screens.
DR ChiTaRS; TUBGCP5; human.
DR GeneWiki; TUBGCP5; -.
DR GenomeRNAi; 114791; -.
DR Pharos; Q96RT8; Tbio.
DR PRO; PR:Q96RT8; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; Q96RT8; protein.
DR Bgee; ENSG00000275835; Expressed in sural nerve and 99 other tissues.
DR ExpressionAtlas; Q96RT8; baseline and differential.
DR Genevisible; Q96RT8; HS.
DR GO; GO:0005813; C:centrosome; IDA:UniProtKB.
DR GO; GO:0005829; C:cytosol; IDA:HPA.
DR GO; GO:0000923; C:equatorial microtubule organizing center; IBA:GO_Central.
DR GO; GO:0000930; C:gamma-tubulin complex; IBA:GO_Central.
DR GO; GO:0000931; C:gamma-tubulin large complex; IDA:UniProtKB.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0000922; C:spindle pole; IEA:InterPro.
DR GO; GO:0043015; F:gamma-tubulin binding; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; IDA:UniProtKB.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; IDA:UniProtKB.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR Gene3D; 1.20.120.1900; -; 1.
DR InterPro; IPR007259; GCP.
DR InterPro; IPR040457; GCP_C.
DR InterPro; IPR042241; GCP_C_sf.
DR InterPro; IPR041470; GCP_N.
DR PANTHER; PTHR19302; PTHR19302; 1.
DR Pfam; PF04130; GCP_C_terminal; 1.
DR Pfam; PF17681; GCP_N_terminal; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Alternative splicing; Cytoplasm; Cytoskeleton; Microtubule;
KW Reference proteome.
FT CHAIN 1..1024
FT /note="Gamma-tubulin complex component 5"
FT /id="PRO_0000078129"
FT REGION 155..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 521..545
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..203
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 528..545
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1010..1024
FT /note="LESLALSLMAGMEQS -> CEYIMLKYFYLCISL (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_047496"
FT VARIANT 662
FT /note="E -> D (in dbSNP:rs35612840)"
FT /id="VAR_049252"
FT CONFLICT 198
FT /note="R -> G (in Ref. 6; AAH71560)"
FT /evidence="ECO:0000305"
FT CONFLICT 994
FT /note="L -> F (in Ref. 3; AK056416)"
FT /evidence="ECO:0000305"
FT HELIX 14..27
FT /evidence="ECO:0007829|PDB:6L81"
FT HELIX 35..49
FT /evidence="ECO:0007829|PDB:6L81"
FT HELIX 58..74
FT /evidence="ECO:0007829|PDB:6L81"
FT HELIX 78..91
FT /evidence="ECO:0007829|PDB:6L81"
FT HELIX 106..117
FT /evidence="ECO:0007829|PDB:6L81"
SQ SEQUENCE 1024 AA; 118321 MW; 21350677DF126CD1 CRC64;
MARHGPPWSR LDAQQERDVR ELVRGVAGLQ DEADPNFQLA LNFAWSNFRF HRFLDVNSHK
IEKTIEGIYE KFVIHSDLSK AASWKRLTEE FLNAPLPSIK EIKTDAHYSI LSLLLCLSDS
PSNSSYVETP RNKEVEKKDD FDWGKYLMED EEMDIGPYMD TPNWSEESEE ENDQQPLSRE
DSGIQVDRTP LEEQDQNRKL DPCISWKDEP DDRSWLEHHV VHQYWTARPS QFPHSLHLHS
NLAAVWDQHL YSSDPLYVPD DRVLVTETQV IRETLWLLSG VKKLFIFQLI DGKVTVRNNI
IVTHLTHSCL RSVLEQIAAY GQVVFRLQEF IDEVMGHSSE SMLPGSGSVP KKSTEAPFRT
YQAFMWALYK YFISFKEELA EIEKCIINND TTITLAIVVD KLAPRLSQLK VLHKVFSTGV
AEVPPDTRNV VRASHLLNTL YKAILEYDNV GEASEQTVSL LFSLWVETVR PYLQTVDEWI
VHGHLWDGAR EFIIQRNKNV PVNHRDFWYA TYTLYSVSEK TENEEKMSDN ASASSGSDQG
PSSRQHTMVS FLKPVLKQII MAGKSMQLLK NLQCAESTTC QAGARDAERK SLYTLFLESV
QSRLRHGEDS TPQVLTEQQA TKENLMKMQS IAESHLELDD VHDPLLAINF ARMYLEQSDF
HEKFAGGDVC VDRSSESVTC QTFELTLRSC LYPHIDKQYL DCCGNLMQTL KKDYRLVEYL
QAMRNFFLME GGDTMYDFYT SIFDKIREKE TWQNVSFLNV QLQEAVGQRY PEDSSRLSIS
FENVDTAKKK LPVHILDGLT LSYKVPWPVD IVISLECQKI YNQVFLLLLQ IKWAKYSLDV
LLFGELVSTA EKPRLKEGLI HEQDTVAQFG PQKEPVRQQI HRMFLLRVKL MHFVNSLHNY
IMTRILHSTG LEFQHQVEEA KDLDQLIKIH YRYLSTIHDR CLLREKVSFV KEAIMKVLNL
ALMFADGWQA GLGTWRMESI EKMESDFKNC HMFLVTILNK AVCRGSFPHL ESLALSLMAG
MEQS
