GCP5_MOUSE
ID GCP5_MOUSE Reviewed; 1024 AA.
AC Q8BKN5; Q3U8X5; Q69Z75; Q8BLJ9; Q8C083;
DT 18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT 18-MAR-2008, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=Gamma-tubulin complex component 5;
DE Short=GCP-5;
GN Name=Tubgcp5; Synonyms=Kiaa1899;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1; 2 AND 3).
RC STRAIN=C57BL/6J;
RC TISSUE=Bone marrow, Medulla oblongata, and Spinal ganglion;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 2-1024 (ISOFORM 1).
RC TISSUE=Embryonic tail;
RX PubMed=15368895; DOI=10.1093/dnares/11.3.205;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Seino S., Nishimura M., Kaisho T., Hoshino K., Kitamura H.,
RA Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: IV.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 11:205-218(2004).
CC -!- FUNCTION: Gamma-tubulin complex is necessary for microtubule nucleation
CC at the centrosome. {ECO:0000250}.
CC -!- SUBUNIT: Gamma-tubulin complex is composed of gamma-tubulin, TUBGCP2,
CC TUBGCP3, TUBGCP4, TUBGCP5 and TUBGCP6. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q8BKN5-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BKN5-2; Sequence=VSP_032040;
CC Name=3;
CC IsoId=Q8BKN5-3; Sequence=VSP_032041;
CC -!- SIMILARITY: Belongs to the TUBGCP family. {ECO:0000305}.
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DR EMBL; AK032031; BAC27661.1; -; mRNA.
DR EMBL; AK044887; BAC32128.1; -; mRNA.
DR EMBL; AK051326; BAC34603.1; -; mRNA.
DR EMBL; AK151454; BAE30414.1; -; mRNA.
DR EMBL; AK152033; BAE30892.1; -; mRNA.
DR EMBL; AK153331; BAE31910.1; -; mRNA.
DR EMBL; AK153357; BAE31930.1; -; mRNA.
DR EMBL; AK173291; BAD32569.1; -; Transcribed_RNA.
DR CCDS; CCDS21314.1; -. [Q8BKN5-1]
DR CCDS; CCDS90246.1; -. [Q8BKN5-2]
DR RefSeq; NP_666302.2; NM_146190.2. [Q8BKN5-1]
DR AlphaFoldDB; Q8BKN5; -.
DR SMR; Q8BKN5; -.
DR BioGRID; 231399; 21.
DR IntAct; Q8BKN5; 17.
DR MINT; Q8BKN5; -.
DR STRING; 10090.ENSMUSP00000032627; -.
DR iPTMnet; Q8BKN5; -.
DR PhosphoSitePlus; Q8BKN5; -.
DR EPD; Q8BKN5; -.
DR MaxQB; Q8BKN5; -.
DR PaxDb; Q8BKN5; -.
DR PeptideAtlas; Q8BKN5; -.
DR PRIDE; Q8BKN5; -.
DR ProteomicsDB; 272944; -. [Q8BKN5-1]
DR ProteomicsDB; 272945; -. [Q8BKN5-2]
DR ProteomicsDB; 272946; -. [Q8BKN5-3]
DR Antibodypedia; 72633; 148 antibodies from 27 providers.
DR DNASU; 233276; -.
DR Ensembl; ENSMUST00000032627; ENSMUSP00000032627; ENSMUSG00000033790. [Q8BKN5-1]
DR Ensembl; ENSMUST00000205796; ENSMUSP00000146111; ENSMUSG00000033790. [Q8BKN5-2]
DR GeneID; 233276; -.
DR KEGG; mmu:233276; -.
DR UCSC; uc009hdd.1; mouse. [Q8BKN5-1]
DR UCSC; uc009hdf.1; mouse. [Q8BKN5-2]
DR CTD; 114791; -.
DR MGI; MGI:2178836; Tubgcp5.
DR VEuPathDB; HostDB:ENSMUSG00000033790; -.
DR eggNOG; KOG4344; Eukaryota.
DR GeneTree; ENSGT00940000155962; -.
DR HOGENOM; CLU_011574_0_0_1; -.
DR InParanoid; Q8BKN5; -.
DR OMA; RTNQFEV; -.
DR OrthoDB; 110079at2759; -.
DR PhylomeDB; Q8BKN5; -.
DR TreeFam; TF329759; -.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR BioGRID-ORCS; 233276; 8 hits in 73 CRISPR screens.
DR PRO; PR:Q8BKN5; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q8BKN5; protein.
DR Bgee; ENSMUSG00000033790; Expressed in spermatocyte and 236 other tissues.
DR ExpressionAtlas; Q8BKN5; baseline and differential.
DR Genevisible; Q8BKN5; MM.
DR GO; GO:0005813; C:centrosome; ISO:MGI.
DR GO; GO:0005829; C:cytosol; ISO:MGI.
DR GO; GO:0000923; C:equatorial microtubule organizing center; IBA:GO_Central.
DR GO; GO:0000930; C:gamma-tubulin complex; IBA:GO_Central.
DR GO; GO:0000931; C:gamma-tubulin large complex; ISO:MGI.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0000922; C:spindle pole; IEA:InterPro.
DR GO; GO:0043015; F:gamma-tubulin binding; IBA:GO_Central.
DR GO; GO:0008017; F:microtubule binding; ISO:MGI.
DR GO; GO:0031122; P:cytoplasmic microtubule organization; IBA:GO_Central.
DR GO; GO:0051321; P:meiotic cell cycle; IBA:GO_Central.
DR GO; GO:0007020; P:microtubule nucleation; ISO:MGI.
DR GO; GO:0000278; P:mitotic cell cycle; IBA:GO_Central.
DR GO; GO:0051225; P:spindle assembly; IBA:GO_Central.
DR Gene3D; 1.20.120.1900; -; 1.
DR InterPro; IPR007259; GCP.
DR InterPro; IPR040457; GCP_C.
DR InterPro; IPR042241; GCP_C_sf.
DR InterPro; IPR041470; GCP_N.
DR PANTHER; PTHR19302; PTHR19302; 1.
DR Pfam; PF04130; GCP_C_terminal; 1.
DR Pfam; PF17681; GCP_N_terminal; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Microtubule;
KW Reference proteome.
FT CHAIN 1..1024
FT /note="Gamma-tubulin complex component 5"
FT /id="PRO_0000323605"
FT REGION 153..203
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 523..545
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 853..873
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 185..203
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 528..545
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 654..716
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032040"
FT VAR_SEQ 977..1024
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_032041"
FT CONFLICT 9
FT /note="S -> L (in Ref. 2; BAD32569)"
FT /evidence="ECO:0000305"
FT CONFLICT 82
FT /note="A -> G (in Ref. 1; BAC27661)"
FT /evidence="ECO:0000305"
FT CONFLICT 172..366
FT /note="Missing (in Ref. 2; BAD32569)"
FT /evidence="ECO:0000305"
FT CONFLICT 225
FT /note="W -> L (in Ref. 1; BAC27661)"
FT /evidence="ECO:0000305"
FT CONFLICT 332
FT /note="D -> Y (in Ref. 1; BAE31930/BAE31910/BAE30892/
FT BAE30414)"
FT /evidence="ECO:0000305"
FT CONFLICT 587
FT /note="A -> E (in Ref. 1; BAC34603)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1024 AA; 117977 MW; F975E340C0A7CE77 CRC64;
MARPKSSGSR MDRQLEHDVC ELVRQVTGLQ DEADPNFQLA LDFVWSNFRF HRFLDVNSHK
VEKTIEGIYE KFTIHSDLNK AASWKRLTKE FLNASLPSTE KIKTDAHYSI LSLLLCLSDS
PSNSNYVETP REKEVEKKDD FDWGKYLMEG EEIGLGPNID TPNWSEDSDD EDAQQPLSRE
DSGIQVDRTP LEEQDHNRKG GPQVCWEDEP DSRSWLEQHV VHQYWTTRRF RIPHSAHLHS
NLAAVWDQHL YSSDPLYVPD DRVVVTETQV IRETLWLLSG VKKMFIFQLI DGKVTVRNNI
IVTHLTHSCL RSVLEQIAAY GQVVFRLQEF IDEVMGHSSE SLPPGNGPIP KKQPDAPFRT
YQAFMWALYK YFINFKEELT DIEKCVISSD TTITLAIVVN KLAPRLAQLK VLDKVFSTGV
AEVPPDTRNV VRASHLLNTL YKAILEYDNV GEASEQTVSL LFSLWVETVR PYLQTVDEWI
VHGHLWDGAR EFIIQRNKNV PVNHRDFWYA TYTLYSVSEK TENEDKVSDS ASASSGSDQG
PSSRQHTMVS FLKPVLKQII MAGKSMQLLK NLNCAEGPAC QAAARDAERK SLYTLFLESI
QLRLQHGEDS APHIVNEDQT TKENLIKMQS IAERHLELDD IHDPLLAINF ARLYLEQSDF
HEKFAGGDIC VDRSSESVTC QTFELTLRSC LYPHIDKQYL HCCGNLMQTL KRDFRLVEYL
QAMRNFFLME GGDTMYDFYT SIFDKIREKE TWQNVSFLNV QLQEAVGQRY PEDSLRLSIS
FENVDTTKKK LPVHILDGLT LSYKVPWPVD IVISVECQKI YNQVFLLLLQ IKWAKYSLDV
LLFGELGNAA ERSQAKEDIP RDQDTPSQFG PPKESLRQQI HRMFLLRVKL MHFVNSLHNY
IMTRILHSTG LEFQHQVEEA KDLDQLIKIH YRYLSTIHDR CLLREKVSFV KEAIMKVLNL
ALMFAEGWQA GLGAWQMESI EKMESDFKNC HMFLVTILNK AVCRGSFPHL ESLALSLMAG
MEQS
