ID   GCS23_FRAAA             Reviewed;         858 AA.
AC   Q0RHA0;
DT   18-MAR-2008, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2006, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Putative glutamate--cysteine ligase 2-3;
DE            EC=6.3.2.2;
DE   AltName: Full=Gamma-glutamylcysteine synthetase 2-3;
DE            Short=GCS 2-3;
DE            Short=Gamma-GCS 2-3;
GN   OrderedLocusNames=FRAAL4489;
OS   Frankia alni (strain ACN14a).
OC   Bacteria; Actinobacteria; Frankiales; Frankiaceae; Frankia.
OX   NCBI_TaxID=326424;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ACN14a;
RX   PubMed=17151343; DOI=10.1101/gr.5798407;
RA   Normand P., Lapierre P., Tisa L.S., Gogarten J.P., Alloisio N.,
RA   Bagnarol E., Bassi C.A., Berry A.M., Bickhart D.M., Choisne N., Couloux A.,
RA   Cournoyer B., Cruveiller S., Daubin V., Demange N., Francino M.P.,
RA   Goltsman E., Huang Y., Kopp O.R., Labarre L., Lapidus A., Lavire C.,
RA   Marechal J., Martinez M., Mastronunzio J.E., Mullin B.C., Niemann J.,
RA   Pujic P., Rawnsley T., Rouy Z., Schenowitz C., Sellstedt A., Tavares F.,
RA   Tomkins J.P., Vallenet D., Valverde C., Wall L.G., Wang Y., Medigue C.,
RA   Benson D.R.;
RT   "Genome characteristics of facultatively symbiotic Frankia sp. strains
RT   reflect host range and host plant biogeography.";
RL   Genome Res. 17:7-15(2007).
CC   -!- FUNCTION: ATP-dependent carboxylate-amine ligase which exhibits weak
CC       glutamate--cysteine ligase activity. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC         cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC   -!- SIMILARITY: In the N-terminal section; belongs to the glutamate--
CC       cysteine ligase type 2 family. YbdK subfamily. {ECO:0000305}.
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DR   EMBL; CT573213; CAJ63131.1; -; Genomic_DNA.
DR   RefSeq; WP_011605610.1; NC_008278.1.
DR   AlphaFoldDB; Q0RHA0; -.
DR   SMR; Q0RHA0; -.
DR   STRING; 326424.FRAAL4489; -.
DR   KEGG; fal:FRAAL4489; -.
DR   eggNOG; COG2170; Bacteria.
DR   eggNOG; COG2308; Bacteria.
DR   HOGENOM; CLU_017048_0_0_11; -.
DR   OMA; DSAWFEH; -.
DR   OrthoDB; 517836at2; -.
DR   Proteomes; UP000000657; Chromosome.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0042398; P:cellular modified amino acid biosynthetic process; IEA:InterPro.
DR   HAMAP; MF_01609; Glu_cys_ligase_2; 1.
DR   InterPro; IPR025841; CP_ATPgrasp_2.
DR   InterPro; IPR006336; GCS2.
DR   InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR   InterPro; IPR011793; YbdK.
DR   Pfam; PF14403; CP_ATPgrasp_2; 1.
DR   Pfam; PF04107; GCS2; 1.
DR   SUPFAM; SSF55931; SSF55931; 1.
DR   TIGRFAMs; TIGR02050; gshA_cyan_rel; 1.
PE   3: Inferred from homology;
KW   ATP-binding; Ligase; Nucleotide-binding; Reference proteome.
FT   CHAIN           1..858
FT                   /note="Putative glutamate--cysteine ligase 2-3"
FT                   /id="PRO_0000323509"
FT   REGION          1..372
FT                   /note="Carboxylate-amine ligase"
FT   REGION          373..858
FT                   /note="Unknown"
SQ   SEQUENCE   858 AA;  92396 MW;  E9CD6E07935765D5 CRC64;
     MSDARNVAVG VEEEFHILDL TTRQLVPRAE EILQRLDKDS FSPELLKSVV ETNSQPTVDL
     LALRSNLLDL RRRLAEAAGE LGLGPAASGT VPILDMDLLD VSRDARYEQM TEDYQIVARE
     QLICGAQVHV DVADRDLAMA VVAWTAPWLP MLLALSASSP YWLGADSGYA SMRTLVWQRW
     PTAGVAGSFQ TAAEYDQLIA DLIKSGVISD PGMVYFDVRP SAHLPTVELR ICDACPDVDN
     VILIAGLFRA LVGRAIEEIE AGGQAPPPRA ELLRAATWRA ARSGLEGDLV DIFGAGPVPA
     RAMLRRMLEE VRPQLERYDD WELIDNLAEQ AAGRGSSALR QRRAFARRGL LTDVADLILA
     ETRDVPPAGA SLGVAPAVSA PDQIAPILLE RYQPAGYDEV VDEHGAVRPQ YRAVMRTLER
     LGPDTLDERV GAREAEQTDR GITFRVNGDS ASRPFPFDIV PRIVAADDWA VLGRGLGQRV
     RALEAFLHDV YGERAAVADG IVPPWVVNDA PGLRHGGRAP GRDAIRITTA GIDLVRGGDG
     GWLVLEDNLR VPSGIAYAVE GRRLAESVLP ELGPPPGILR LGTVPALLHD ALVAAAPPAV
     TGEPAVAVLT SGPADSAYFE HAMLAEEMGV PLVEPGALLV DDDVAYRVDD GRRRRVDVLY
     RRIDEDELFA APGADGAPLG PALLRAVRAG QVSVANAPGN GIGDDKVVYA YVPRMVTYYL
     GEQPVLDDVP TYVCGDPEQC EHVLANLDQL VVKPVDGFGG SGVVIGPHAE PYQLTAVREQ
     ILADPRRWIG QEVVALSTHP TWHDSHLEPC AVDLRVFVYA GVEPVVVPAA LSRVAPPGSL
     IVNSSQGGGS KDTWIPRR