ID   GCS2_ARATH              Reviewed;         789 AA.
AC   Q84M89; O48699;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Alpha-glucosidase 2;
DE            Short=Glucosidase 2;
DE            EC=3.2.1.-;
GN   Name=GCS2; OrderedLocusNames=At1g24320; ORFNames=F3I6.26;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RA   Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA   Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA   Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA   Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA   Shinozaki K.;
RT   "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL   Submitted (JUL-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 63 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAC00593.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AC002396; AAC00593.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE30516.1; -; Genomic_DNA.
DR   EMBL; BT006494; AAP21302.1; -; mRNA.
DR   EMBL; AK227426; BAE99430.1; -; mRNA.
DR   PIR; T00663; T00663.
DR   RefSeq; NP_173842.2; NM_102278.6.
DR   AlphaFoldDB; Q84M89; -.
DR   SMR; Q84M89; -.
DR   STRING; 3702.AT1G24320.1; -.
DR   CAZy; GH63; Glycoside Hydrolase Family 63.
DR   PaxDb; Q84M89; -.
DR   PRIDE; Q84M89; -.
DR   ProteomicsDB; 222024; -.
DR   EnsemblPlants; AT1G24320.1; AT1G24320.1; AT1G24320.
DR   GeneID; 839048; -.
DR   Gramene; AT1G24320.1; AT1G24320.1; AT1G24320.
DR   KEGG; ath:AT1G24320; -.
DR   Araport; AT1G24320; -.
DR   TAIR; locus:2032462; AT1G24320.
DR   eggNOG; KOG2161; Eukaryota.
DR   HOGENOM; CLU_007380_1_0_1; -.
DR   InParanoid; Q84M89; -.
DR   OMA; IAPDEFH; -.
DR   OrthoDB; 278028at2759; -.
DR   PhylomeDB; Q84M89; -.
DR   BioCyc; ARA:AT1G24320-MON; -.
DR   UniPathway; UPA00280; -.
DR   PRO; PR:Q84M89; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; Q84M89; baseline and differential.
DR   Genevisible; Q84M89; AT.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR   GO; GO:0004573; F:Glc3Man9GlcNAc2 oligosaccharide glucosidase activity; IBA:GO_Central.
DR   GO; GO:0009311; P:oligosaccharide metabolic process; IEA:InterPro.
DR   GO; GO:0006487; P:protein N-linked glycosylation; IBA:GO_Central.
DR   Gene3D; 1.50.10.10; -; 1.
DR   Gene3D; 2.70.98.110; -; 1.
DR   InterPro; IPR008928; 6-hairpin_glycosidase_sf.
DR   InterPro; IPR012341; 6hp_glycosidase-like_sf.
DR   InterPro; IPR031335; Glyco_hydro_63_C.
DR   InterPro; IPR031631; Glyco_hydro_63N.
DR   InterPro; IPR038518; Glyco_hydro_63N_sf.
DR   InterPro; IPR004888; Glycoside_hydrolase_63.
DR   PANTHER; PTHR10412; PTHR10412; 1.
DR   Pfam; PF03200; Glyco_hydro_63; 1.
DR   Pfam; PF16923; Glyco_hydro_63N; 1.
DR   SUPFAM; SSF48208; SSF48208; 1.
PE   2: Evidence at transcript level;
KW   Glycosidase; Hydrolase; Reference proteome.
FT   CHAIN           1..789
FT                   /note="Alpha-glucosidase 2"
FT                   /id="PRO_0000420921"
FT   REGION          1..24
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          512..531
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   789 AA;  90609 MW;  F01D5E750921D159 CRC64;
     MTGLSPSGDI KPLLDDESQR PRVITPFPSP KLTDLSMFQG EHKESLYWGT YRPQMYFGVR
     ARTPKSLVAG LMWLVMKDGK PVMRHFCENS NDLKSFGWKE HNGRDFGRQE LFEQNMVLET
     SFVKSEEGSL GYGGDWSIRI NVQNMLNDDE VKRSTHLFFY LADEGCNGVN LGKDVLRLKE
     SSVLASGSRQ DVGNWQMHLK SQNHLETHYC GFKTPDIVNL SGLVQQNLTA QEEKSGLLQL
     SDSSEDSSNI YVFQISTTNQ STIDIAFVSG IKEETSNMEN RIMSLTGLPL SSLLEEKHIA
     FNAKFNECFN LSDKLDPETL VVGKAAIGNM LGGIGYFYGQ SKIHFPKSPL AKSEDDFLLY
     WPAELYTAVP SRPRFPRGFL WDEGFHQLLI WRWDVHITLE IVGNWLDLMN IDGWIPREQV
     LGAEALSKIP KQYVVQFPSN GNPPTLLLVI RDLINGIRTE KFNEADRDKI LSFLDRAFVR
     LDAWFKWFNT SQIGKEKGSY YWHGRDNITN RELNPQSLSS GLDDYPRASH PNEDERHVDL
     RCWMYLAADS MNSIQEFMGK NDILVTEDYS SIAKLLSDFT LLNQMHYDKD HGAYLDFGNH
     TEEVRLVWKE VIHEDGHLSR ELVRETYGKP ELRLVPHIGY VSFFPFMFRI IPADSSILDK
     QLDLISNGNI VWSDYGLLSL AKTSSLYMKY NSEHQAPYWR GAIWMNMNYM ILSSLHHYST
     VDGPYNSKAR TIYEELRSNL IRNVVRNYDQ TGIIWEHYDQ TKGTGEGARV FTGWSALILL
     IMSEEYPLF