GCS2_MYCUA
ID GCS2_MYCUA Reviewed; 392 AA.
AC A0PNK6;
DT 12-JUN-2007, integrated into UniProtKB/Swiss-Prot.
DT 09-JAN-2007, sequence version 1.
DT 25-MAY-2022, entry version 78.
DE RecName: Full=Putative glutamate--cysteine ligase 2 {ECO:0000255|HAMAP-Rule:MF_01609};
DE EC=6.3.2.2 {ECO:0000255|HAMAP-Rule:MF_01609};
DE AltName: Full=Gamma-glutamylcysteine synthetase 2 {ECO:0000255|HAMAP-Rule:MF_01609};
DE Short=GCS 2 {ECO:0000255|HAMAP-Rule:MF_01609};
DE Short=Gamma-GCS 2 {ECO:0000255|HAMAP-Rule:MF_01609};
GN OrderedLocusNames=MUL_1380;
OS Mycobacterium ulcerans (strain Agy99).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium.
OX NCBI_TaxID=362242;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Agy99;
RX PubMed=17210928; DOI=10.1101/gr.5942807;
RA Stinear T.P., Seemann T., Pidot S., Frigui W., Reysset G., Garnier T.,
RA Meurice G., Simon D., Bouchier C., Ma L., Tichit M., Porter J.L., Ryan J.,
RA Johnson P.D.R., Davies J.K., Jenkin G.A., Small P.L.C., Jones L.M.,
RA Tekaia F., Laval F., Daffe M., Parkhill J., Cole S.T.;
RT "Reductive evolution and niche adaptation inferred from the genome of
RT Mycobacterium ulcerans, the causative agent of Buruli ulcer.";
RL Genome Res. 17:192-200(2007).
CC -!- FUNCTION: ATP-dependent carboxylate-amine ligase which exhibits weak
CC glutamate--cysteine ligase activity. {ECO:0000255|HAMAP-Rule:MF_01609}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-cysteine + L-glutamate = ADP + gamma-L-glutamyl-L-
CC cysteine + H(+) + phosphate; Xref=Rhea:RHEA:13285, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:29985, ChEBI:CHEBI:30616, ChEBI:CHEBI:35235,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:58173, ChEBI:CHEBI:456216; EC=6.3.2.2;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01609};
CC -!- SIMILARITY: Belongs to the glutamate--cysteine ligase type 2 family.
CC YbdK subfamily. {ECO:0000255|HAMAP-Rule:MF_01609}.
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DR EMBL; CP000325; ABL03925.1; -; Genomic_DNA.
DR AlphaFoldDB; A0PNK6; -.
DR SMR; A0PNK6; -.
DR STRING; 362242.MUL_1380; -.
DR EnsemblBacteria; ABL03925; ABL03925; MUL_1380.
DR KEGG; mul:MUL_1380; -.
DR eggNOG; COG2170; Bacteria.
DR HOGENOM; CLU_044848_1_0_11; -.
DR OMA; LIFGLHV; -.
DR Proteomes; UP000000765; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004357; F:glutamate-cysteine ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0042398; P:cellular modified amino acid biosynthetic process; IEA:InterPro.
DR HAMAP; MF_01609; Glu_cys_ligase_2; 1.
DR InterPro; IPR006336; GCS2.
DR InterPro; IPR014746; Gln_synth/guanido_kin_cat_dom.
DR InterPro; IPR011793; YbdK.
DR Pfam; PF04107; GCS2; 1.
DR SUPFAM; SSF55931; SSF55931; 1.
DR TIGRFAMs; TIGR02050; gshA_cyan_rel; 1.
PE 3: Inferred from homology;
KW ATP-binding; Ligase; Nucleotide-binding.
FT CHAIN 1..392
FT /note="Putative glutamate--cysteine ligase 2"
FT /id="PRO_0000291500"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 392 AA; 43577 MW; FB6162DCDE5144B6 CRC64;
MMPVSGWRAV SSAPASSSAG RTANRIDFAR SPRPTVGVEW EFALVDAETR DLSNEATAVI
AEIGENPRVH KELLRNTVEV VSGVCGSAGE AIEDLRTTLG PARRIVHARG MELFCEGTHP
FAQWSTQKLT DAPRYAELIK RTQWWGRQML IWGVHVHVGV SSANKVMPII SALLNYYPHL
LALSASSPWW GGEDTGYASN RAMMFQQLPT AGLPFQFQTW SEFEGFVYDQ KKTGIIDHIN
EIRWDIRPSP HLGTIELRIC DGVSNLRELG ALVALMHCLV VDLDRRLDAG ETLPTMPPWH
VQENKWRAAR YGLDAVIILD AESNERLVTD DLDDVLTRLE PVARSLSCAD ELAAVAEIPR
VGASYQRQRR VAEEHDGDLR AVVDALIAEL DI