ID   GCSH5_DICDI             Reviewed;         163 AA.
AC   Q54JU0;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 76.
DE   RecName: Full=Glycine cleavage system H-like protein gcvH5, mitochondrial;
DE   Flags: Precursor;
GN   Name=gcvH5; Synonyms=gcvH; ORFNames=DDB_G0287861;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GcvH family. {ECO:0000305}.
CC   -!- CAUTION: Ser-94 is present instead of the conserved Lys which is
CC       expected to bind the lipoyl cofactor. {ECO:0000305}.
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DR   EMBL; AAFI02000104; EAL63577.1; -; Genomic_DNA.
DR   RefSeq; XP_637062.1; XM_631970.1.
DR   AlphaFoldDB; Q54JU0; -.
DR   SMR; Q54JU0; -.
DR   STRING; 44689.DDB0231215; -.
DR   PaxDb; Q54JU0; -.
DR   EnsemblProtists; EAL63577; EAL63577; DDB_G0287861.
DR   GeneID; 8626316; -.
DR   KEGG; ddi:DDB_G0287861; -.
DR   dictyBase; DDB_G0287861; gcvH5.
DR   eggNOG; KOG3373; Eukaryota.
DR   HOGENOM; CLU_1630090_0_0_1; -.
DR   InParanoid; Q54JU0; -.
DR   PhylomeDB; Q54JU0; -.
DR   PRO; PR:Q54JU0; -.
DR   Proteomes; UP000002195; Chromosome 5.
DR   GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR   GO; GO:0005960; C:glycine cleavage complex; IEA:InterPro.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IBA:GO_Central.
DR   GO; GO:0009249; P:protein lipoylation; IBA:GO_Central.
DR   CDD; cd06848; GCS_H; 1.
DR   InterPro; IPR000089; Biotin_lipoyl.
DR   InterPro; IPR002930; GCV_H.
DR   InterPro; IPR033753; GCV_H/Fam206.
DR   InterPro; IPR011053; Single_hybrid_motif.
DR   PANTHER; PTHR11715; PTHR11715; 1.
DR   Pfam; PF01597; GCV_H; 1.
DR   SUPFAM; SSF51230; SSF51230; 1.
DR   PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
PE   3: Inferred from homology;
KW   Mitochondrion; Reference proteome; Transit peptide.
FT   TRANSIT         1..23
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           24..163
FT                   /note="Glycine cleavage system H-like protein gcvH5,
FT                   mitochondrial"
FT                   /id="PRO_0000328551"
FT   DOMAIN          50..136
FT                   /note="Lipoyl-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01066"
SQ   SEQUENCE   163 AA;  18922 MW;  61C5F5215C431B92 CRC64;
     MFLFKTTNNL RKSLSNKFFC TRYSKNHLWI SINNNNNSNN NNNNNNNIII GTLGLTENGP
     INKFDDVLYI KFPIIKDENQ VDDEPLTISL DTISDQILLN SPIRNCKLIS INQDVLDYPN
     YINSSPMSKG WLCKIKFSNI NDFNSLMNKN EYDNYCKNKI IKI