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GCSH_FLATR
ID   GCSH_FLATR              Reviewed;         165 AA.
AC   P46485; Q39733;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 92.
DE   RecName: Full=Glycine cleavage system H protein, mitochondrial;
DE   AltName: Full=Lipoyl-bearing H protein;
DE   Flags: Precursor;
GN   Name=GDCSH; Synonyms=GCDH, GCSH;
OS   Flaveria trinervia (Clustered yellowtops) (Oedera trinervia).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC   Heliantheae alliance; Tageteae; Flaveria.
OX   NCBI_TaxID=4227;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2), ALTERNATIVE
RP   SPLICING, AND TISSUE SPECIFICITY.
RC   TISSUE=Leaf;
RX   PubMed=7550380; DOI=10.1046/j.1365-313x.1995.08030435.x;
RA   Kopriva S., Cossu R., Bauwe H.;
RT   "Alternative splicing results in two different transcripts for H-protein of
RT   the glycine cleavage system in the C4 species Flaveria trinervia.";
RL   Plant J. 8:435-441(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Leaf;
RX   PubMed=8552027; DOI=10.1007/bf00290242;
RA   Kopriva S., Bauwe H.;
RT   "H-protein of glycine decarboxylase is encoded by multigene families in
RT   Flaveria pringlei and F. cronquistii (Asteraceae).";
RL   Mol. Gen. Genet. 249:111-116(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [MRNA] OF 1-143 (ISOFORM 1), AND ALTERNATIVE SPLICING.
RA   Kopriva S., Chu C.C., Bauwe H.;
RT   "Molecular phylogeny of Flaveria as deduced from the analysis of H-protein
RT   nucleotide sequences.";
RL   Plant Cell Environ. 19:1028-1036(1996).
RN   [4]
RP   ALTERNATIVE SPLICING.
RC   TISSUE=Leaf;
RX   PubMed=8771790; DOI=10.1046/j.1365-313x.1996.10020369.x;
RA   Kopriva S., Chu C.-C., Bauwe H.;
RT   "H-protein of the glycine cleavage system in Flaveria: alternative splicing
RT   of the pre-mRNA occurs exclusively in advanced C4 species of the genus.";
RL   Plant J. 10:369-373(1996).
CC   -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC       glycine. The H protein shuttles the methylamine group of glycine from
CC       the P protein to the T protein.
CC   -!- COFACTOR:
CC       Name=(R)-lipoate; Xref=ChEBI:CHEBI:83088; Evidence={ECO:0000250};
CC       Note=Binds 1 lipoyl cofactor covalently. {ECO:0000250};
CC   -!- SUBUNIT: The glycine cleavage system is composed of four components
CC       that only loosely associate: the P protein (EC 1.4.4.2), the T protein
CC       (EC 2.1.2.10), the L protein (EC 1.8.1.4) and the lipoyl-bearing H
CC       protein.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC         Comment=This alternative splicing is restricted to C4 species of
CC         Flaveria. {ECO:0000269|PubMed:7550380, ECO:0000269|PubMed:8771790,
CC         ECO:0000269|Ref.3};
CC       Name=1;
CC         IsoId=P46485-1; Sequence=Displayed;
CC       Name=2;
CC         IsoId=P46485-2; Sequence=VSP_040391;
CC   -!- TISSUE SPECIFICITY: Expressed in roots, stems and leaves.
CC       {ECO:0000269|PubMed:7550380}.
CC   -!- MISCELLANEOUS: [Isoform 1]: Major isoform in stems and leaves.
CC   -!- MISCELLANEOUS: [Isoform 2]: Major isoform in roots. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the GcvH family. {ECO:0000305}.
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DR   EMBL; Z37523; CAA85760.1; -; mRNA.
DR   EMBL; Z48797; CAA88734.1; -; Genomic_DNA.
DR   EMBL; Z70303; CAA94316.1; -; mRNA.
DR   PIR; S49232; S49232.
DR   PIR; S57665; S57665.
DR   AlphaFoldDB; P46485; -.
DR   SMR; P46485; -.
DR   GO; GO:0005960; C:glycine cleavage complex; IEA:InterPro.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IEA:InterPro.
DR   CDD; cd06848; GCS_H; 1.
DR   HAMAP; MF_00272; GcvH; 1.
DR   InterPro; IPR003016; 2-oxoA_DH_lipoyl-BS.
DR   InterPro; IPR000089; Biotin_lipoyl.
DR   InterPro; IPR002930; GCV_H.
DR   InterPro; IPR033753; GCV_H/Fam206.
DR   InterPro; IPR017453; GCV_H_sub.
DR   InterPro; IPR011053; Single_hybrid_motif.
DR   PANTHER; PTHR11715; PTHR11715; 1.
DR   Pfam; PF01597; GCV_H; 1.
DR   SUPFAM; SSF51230; SSF51230; 1.
DR   TIGRFAMs; TIGR00527; gcvH; 1.
DR   PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR   PROSITE; PS00189; LIPOYL; 1.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Lipoyl; Mitochondrion; Transit peptide.
FT   TRANSIT         1..31
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250"
FT   CHAIN           32..165
FT                   /note="Glycine cleavage system H protein, mitochondrial"
FT                   /id="PRO_0000010735"
FT   DOMAIN          55..137
FT                   /note="Lipoyl-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01066"
FT   MOD_RES         96
FT                   /note="N6-lipoyllysine"
FT                   /evidence="ECO:0000250, ECO:0000255|PROSITE-
FT                   ProRule:PRU01066"
FT   VAR_SEQ         34..35
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:7550380"
FT                   /id="VSP_040391"
SQ   SEQUENCE   165 AA;  17674 MW;  0BB544B371856BAB CRC64;
     MALRIWASST AKALRLSSAS RPHFSPLFRC FSSAAVLDGL KYANSHEWVK HEGSVATIGI
     TDHAQDHLGE VVFVELPEVG GSVTKATGFG AVESVKATSD VNSPISGEIV EVNSKLTKTP
     GLINKSPYED GWMIKVKPSN PSELDSLMGP KEYTKFCEEE GAAAH
 
 
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