ID   GCSH_STAAM              Reviewed;         126 AA.
AC   P64213; Q99VH3;
DT   11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT   11-OCT-2004, sequence version 1.
DT   03-AUG-2022, entry version 105.
DE   RecName: Full=Glycine cleavage system H protein {ECO:0000255|HAMAP-Rule:MF_00272, ECO:0000303|PubMed:26166706};
DE   AltName: Full=Octanoyl/lipoyl carrier protein {ECO:0000255|HAMAP-Rule:MF_00272};
GN   Name=gcvH {ECO:0000255|HAMAP-Rule:MF_00272}; OrderedLocusNames=SAV0833;
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   LIPOYLATION.
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=26166706; DOI=10.1016/j.molcel.2015.06.013;
RA   Rack J.G., Morra R., Barkauskaite E., Kraehenbuehl R., Ariza A., Qu Y.,
RA   Ortmayer M., Leidecker O., Cameron D.R., Matic I., Peleg A.Y., Leys D.,
RA   Traven A., Ahel I.;
RT   "Identification of a class of protein ADP-ribosylating sirtuins in
RT   microbial pathogens.";
RL   Mol. Cell 59:309-320(2015).
CC   -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC       glycine. The H protein shuttles the methylamine group of glycine from
CC       the P protein to the T protein. {ECO:0000255|HAMAP-Rule:MF_00272}.
CC   -!- FUNCTION: Is also involved in protein lipoylation via its role as an
CC       octanoyl/lipoyl carrier protein intermediate. {ECO:0000255|HAMAP-
CC       Rule:MF_00272}.
CC   -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC       T, L and H. {ECO:0000255|HAMAP-Rule:MF_00272}.
CC   -!- PTM: Is lipoylated by LplA1 (SAV1028). {ECO:0000269|PubMed:26166706}.
CC   -!- SIMILARITY: Belongs to the GcvH family. {ECO:0000255|HAMAP-
CC       Rule:MF_00272}.
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DR   EMBL; BA000017; BAB56995.1; -; Genomic_DNA.
DR   RefSeq; WP_000290491.1; NC_002758.2.
DR   AlphaFoldDB; P64213; -.
DR   SMR; P64213; -.
DR   PaxDb; P64213; -.
DR   EnsemblBacteria; BAB56995; BAB56995; SAV0833.
DR   KEGG; sav:SAV0833; -.
DR   HOGENOM; CLU_097408_2_0_9; -.
DR   OMA; YRDSHEW; -.
DR   PhylomeDB; P64213; -.
DR   BioCyc; SAUR158878:SAV_RS04545-MON; -.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0005960; C:glycine cleavage complex; IEA:InterPro.
DR   GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IEA:UniProtKB-UniRule.
DR   GO; GO:0009249; P:protein lipoylation; IEA:UniProtKB-UniRule.
DR   CDD; cd06848; GCS_H; 1.
DR   HAMAP; MF_00272; GcvH; 1.
DR   InterPro; IPR003016; 2-oxoA_DH_lipoyl-BS.
DR   InterPro; IPR000089; Biotin_lipoyl.
DR   InterPro; IPR002930; GCV_H.
DR   InterPro; IPR033753; GCV_H/Fam206.
DR   InterPro; IPR017453; GCV_H_sub.
DR   InterPro; IPR011053; Single_hybrid_motif.
DR   PANTHER; PTHR11715; PTHR11715; 1.
DR   Pfam; PF01597; GCV_H; 1.
DR   SUPFAM; SSF51230; SSF51230; 1.
DR   TIGRFAMs; TIGR00527; gcvH; 1.
DR   PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
DR   PROSITE; PS00189; LIPOYL; 1.
PE   3: Inferred from homology;
KW   Lipoyl.
FT   CHAIN           1..126
FT                   /note="Glycine cleavage system H protein"
FT                   /id="PRO_0000166247"
FT   DOMAIN          22..104
FT                   /note="Lipoyl-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01066"
FT   MOD_RES         63
FT                   /note="N6-lipoyllysine"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00272"
SQ   SEQUENCE   126 AA;  14081 MW;  BC20394A8529E4CF CRC64;
     MAVPNELKYS KEHEWVKVEG NVATIGITEY AQSELGDIVF VELPETDDEI NEGDTFGSVE
     SVKTVSELYA PISGKVVEVN EELEDSPEFV NESPYEKAWM VKVEISDESQ LEALLTAEKY
     SEMIGE