ID   ALMA4_EMIHU             Reviewed;         532 AA.
AC   R1ERP2;
DT   16-SEP-2015, integrated into UniProtKB/Swiss-Prot.
DT   26-JUN-2013, sequence version 1.
DT   03-AUG-2022, entry version 27.
DE   RecName: Full=Dimethylsulfonioproprionate lyase 4 {ECO:0000305};
DE            Short=DMSP lyase 4 {ECO:0000305};
DE            EC=4.4.1.3 {ECO:0000250|UniProtKB:P0DN21};
DE   AltName: Full=Dimethylpropiothetin dethiomethylase 4 {ECO:0000305};
GN   Name=ALMA4 {ECO:0000303|PubMed:26113722};
GN   ORFNames=EMIHUDRAFT_115376 {ECO:0000312|EMBL:EOD25646.1};
OS   Emiliania huxleyi (Coccolithophore) (Pontosphaera huxleyi).
OC   Eukaryota; Haptista; Haptophyta; Prymnesiophyceae; Isochrysidales;
OC   Noelaerhabdaceae; Emiliania.
OX   NCBI_TaxID=2903;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CCMP1516;
RX   PubMed=23760476; DOI=10.1038/nature12221;
RA   Read B.A., Kegel J., Klute M.J., Kuo A., Lefebvre S.C., Maumus F.,
RA   Mayer C., Miller J., Monier A., Salamov A., Young J., Aguilar M.,
RA   Claverie J.M., Frickenhaus S., Gonzalez K., Herman E.K., Lin Y.C.,
RA   Napier J., Ogata H., Sarno A.F., Shmutz J., Schroeder D., de Vargas C.,
RA   Verret F., von Dassow P., Valentin K., Van de Peer Y., Wheeler G.,
RA   Dacks J.B., Delwiche C.F., Dyhrman S.T., Glockner G., John U., Richards T.,
RA   Worden A.Z., Zhang X., Grigoriev I.V., Allen A.E., Bidle K., Borodovsky M.,
RA   Bowler C., Brownlee C., Cock J.M., Elias M., Gladyshev V.N., Groth M.,
RA   Guda C., Hadaegh A., Iglesias-Rodriguez M.D., Jenkins J., Jones B.M.,
RA   Lawson T., Leese F., Lindquist E., Lobanov A., Lomsadze A., Malik S.B.,
RA   Marsh M.E., Mackinder L., Mock T., Mueller-Roeber B., Pagarete A.,
RA   Parker M., Probert I., Quesneville H., Raines C., Rensing S.A.,
RA   Riano-Pachon D.M., Richier S., Rokitta S., Shiraiwa Y., Soanes D.M.,
RA   van der Giezen M., Wahlund T.M., Williams B., Wilson W., Wolfe G.,
RA   Wurch L.L.;
RT   "Pan genome of the phytoplankton Emiliania underpins its global
RT   distribution.";
RL   Nature 499:209-213(2013).
RN   [2]
RP   IDENTIFICATION.
RX   PubMed=26113722; DOI=10.1126/science.aab1586;
RA   Alcolombri U., Ben-Dor S., Feldmesser E., Levin Y., Tawfik D.S., Vardi A.;
RT   "Identification of the algal dimethyl sulfide-releasing enzyme: A missing
RT   link in the marine sulfur cycle.";
RL   Science 348:1466-1469(2015).
CC   -!- FUNCTION: Mediates cleavage of dimethylsulfonioproprionate (DMSP) into
CC       dimethyl sulfide (DMS) and acrylate. DMS is the principal form by which
CC       sulfur is transported from oceans to the atmosphere and is a key
CC       component of the ocean sulfur cycle. {ECO:0000250|UniProtKB:P0DN21}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=S,S-dimethyl-beta-propiothetin = acrylate + dimethyl sulfide +
CC         H(+); Xref=Rhea:RHEA:19965, ChEBI:CHEBI:15378, ChEBI:CHEBI:16457,
CC         ChEBI:CHEBI:17437, ChEBI:CHEBI:37080; EC=4.4.1.3;
CC         Evidence={ECO:0000250|UniProtKB:P0DN21};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000250|UniProtKB:P0DN21}.
CC   -!- SIMILARITY: Belongs to the aspartate/glutamate racemases family. ALMA1
CC       subfamily. {ECO:0000305}.
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DR   EMBL; KB865269; EOD25646.1; -; Genomic_DNA.
DR   RefSeq; XP_005778075.1; XM_005778018.1.
DR   AlphaFoldDB; R1ERP2; -.
DR   SMR; R1ERP2; -.
DR   GeneID; 17271191; -.
DR   KEGG; ehx:EMIHUDRAFT_115376; -.
DR   eggNOG; ENOG502RZWQ; Eukaryota.
DR   HOGENOM; CLU_567962_0_0_1; -.
DR   Proteomes; UP000013827; Unassembled WGS sequence.
DR   GO; GO:0047869; F:dimethylpropiothetin dethiomethylase activity; IEA:UniProtKB-EC.
PE   3: Inferred from homology;
KW   Lyase; Reference proteome.
FT   CHAIN           1..532
FT                   /note="Dimethylsulfonioproprionate lyase 4"
FT                   /id="PRO_0000433891"
SQ   SEQUENCE   532 AA;  57839 MW;  C65E7F71BEC8BEAE CRC64;
     MGCTHSKEHS TANKPPAVGI LRCHGARWMP AQAVVGTNSY LFRTVHAEVK GLRYLDVRAG
     KELSVSQKKA LKEAVKELDA EGVVAITGDC GSFVHYQTAV RRMTKTPAVL SPLLQAPLLA
     TMYMKEETIL VLTNDSSDYD QAALESNLVE IGLSQEDAER FVIQGLQHIE GFSTSEVADM
     SDERTWAMVD TSERLRLEIM STIEAAKKAN PSLRAILLES TLLPSFSDSM RQTRGVPVFD
     AITLADYLAA ASTDNPRFGS NIDASVWSSA MERANVMDEL SQRATPAIGI LRIDYSYPPA
     PGDVDYPGSY YYRTVQEVAA GLTFEAAQEG RPLTAQQREA MEGAIRRLEA AKGVVGITGD
     CGFLMNYQVD ARRMSHLPCF ISAMMQCHML AASFAADEEF LVLTANGKSL APKFGEMLSL
     AHVTRPEDQA RFHILGCEDV DGFDAVAKGE AVDVARVTPG IVALAKAAAA RRPKVRAVLL
     ECTELPPYAD ALRHALRIPV LDAITLVDFV HSASTDNPAF GVDFQKSSKV FV