ID   GCST_FLAAN              Reviewed;         407 AA.
AC   O49849;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Aminomethyltransferase, mitochondrial;
DE            EC=2.1.2.10;
DE   AltName: Full=Glycine cleavage system T protein;
DE            Short=GCVT;
DE   Flags: Precursor;
GN   Name=GDCST;
OS   Flaveria anomala (Yellowtops).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; campanulids; Asterales; Asteraceae; Asteroideae;
OC   Heliantheae alliance; Tageteae; Flaveria.
OX   NCBI_TaxID=35877;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   TISSUE=Leaf;
RA   Nan Q., Chu C.-C., Bauwe H.;
RT   "The GDCST gene encoding T-protein of the glycine cleavage system in the
RT   C3-C4 intermediate plant Flaveria anomala.";
RL   (er) Plant Gene Register PGR98-006(1998).
CC   -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC       glycine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-
CC         aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-
CC         5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +
CC         NH4(+); Xref=Rhea:RHEA:16945, Rhea:RHEA-COMP:10475, Rhea:RHEA-
CC         COMP:10492, ChEBI:CHEBI:15636, ChEBI:CHEBI:28938, ChEBI:CHEBI:57453,
CC         ChEBI:CHEBI:83100, ChEBI:CHEBI:83143; EC=2.1.2.10;
CC   -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC       T, L and H.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000305}.
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DR   EMBL; Z71184; CAA94902.1; -; Genomic_DNA.
DR   AlphaFoldDB; O49849; -.
DR   SMR; O49849; -.
DR   PRIDE; O49849; -.
DR   GO; GO:0005739; C:mitochondrion; IEA:UniProtKB-SubCell.
DR   GO; GO:0004047; F:aminomethyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006546; P:glycine catabolic process; IEA:InterPro.
DR   Gene3D; 3.30.1360.120; -; 1.
DR   InterPro; IPR006223; GCS_T.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   PANTHER; PTHR43757; PTHR43757; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   SUPFAM; SSF101790; SSF101790; 1.
DR   TIGRFAMs; TIGR00528; gcvT; 1.
PE   3: Inferred from homology;
KW   Aminotransferase; Mitochondrion; Transferase; Transit peptide.
FT   TRANSIT         1..29
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..407
FT                   /note="Aminomethyltransferase, mitochondrial"
FT                   /id="PRO_0000010759"
FT   BINDING         234
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         265
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         403
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   407 AA;  44279 MW;  7F1BE2896CDD1A59 CRC64;
     MRGGLWQLGQ SITRRLGQSD KKTIVRRCYA SEADLKKTVL YDFHVAHGGK MVPFAGWSMP
     IQYKDSIMDS TINCRENGSL FDVSHMCGLS LKGKDCVAFL EKLVVADVAG LAPGTGSLTV
     FTNEKGGAID DSVITKVTDD HIYLVVNAGC RDKDLAHIEE HMKAFKAKGG DVSWHIYDER
     SLLALQGPLA GSTLQHLTKE DLSKMYFGDF RIIDINGSKC FLTRTGYTGE DGFEISVPSE
     NAVDLAKAIL EKSEGKVRLT GLGARDSLRL EAGLCLYGND MEQHITPVEA GLTWAIGKRR
     RAEGGFLGAD VILKQIADGP AIRRVGLFST GPPARSHSEI QNEKGENIGE VTSGGFSPCL
     KKNIGMGYVK SGLHKPGTKL KIVIRGKTYE GSVTKMPFVP TKYYKPA