GCST_MOUSE
ID GCST_MOUSE Reviewed; 403 AA.
AC Q8CFA2;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Aminomethyltransferase, mitochondrial {ECO:0000305};
DE EC=2.1.2.10 {ECO:0000250|UniProtKB:P28337, ECO:0000250|UniProtKB:P48728};
DE AltName: Full=Glycine cleavage system T protein {ECO:0000250|UniProtKB:P48728};
DE Short=GCVT;
DE Flags: Precursor;
GN Name=Amt {ECO:0000312|MGI:MGI:3646700};
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=129S6/SvEvTac;
RX PubMed=12487019; DOI=10.1080/1042517021000021572;
RA Backofen B., Leeb T.;
RT "Genomic organization of the murine aminomethyltransferase gene (Amt).";
RL DNA Seq. 13:179-183(2002).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Kidney, Liver, and Pancreas;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [3]
RP SUCCINYLATION [LARGE SCALE ANALYSIS] AT LYS-368, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
CC -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC glycine. {ECO:0000250|UniProtKB:P48728}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-
CC aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-
CC 5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +
CC NH4(+); Xref=Rhea:RHEA:16945, Rhea:RHEA-COMP:10475, Rhea:RHEA-
CC COMP:10492, ChEBI:CHEBI:15636, ChEBI:CHEBI:28938, ChEBI:CHEBI:57453,
CC ChEBI:CHEBI:83100, ChEBI:CHEBI:83143; EC=2.1.2.10;
CC Evidence={ECO:0000250|UniProtKB:P48728};
CC -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC T, L and H. {ECO:0000250|UniProtKB:P48728}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:P48728}.
CC -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000305}.
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DR EMBL; AJ437692; CAD26917.1; -; Genomic_DNA.
DR CCDS; CCDS23519.1; -.
DR RefSeq; NP_001013836.1; NM_001013814.1.
DR AlphaFoldDB; Q8CFA2; -.
DR SMR; Q8CFA2; -.
DR BioGRID; 241804; 4.
DR STRING; 10090.ENSMUSP00000035230; -.
DR iPTMnet; Q8CFA2; -.
DR PhosphoSitePlus; Q8CFA2; -.
DR SwissPalm; Q8CFA2; -.
DR jPOST; Q8CFA2; -.
DR MaxQB; Q8CFA2; -.
DR PaxDb; Q8CFA2; -.
DR PRIDE; Q8CFA2; -.
DR ProteomicsDB; 267782; -.
DR DNASU; 434437; -.
DR Ensembl; ENSMUST00000035230; ENSMUSP00000035230; ENSMUSG00000032607.
DR GeneID; 434437; -.
DR KEGG; mmu:434437; -.
DR UCSC; uc009rpc.1; mouse.
DR CTD; 275; -.
DR MGI; MGI:3646700; Amt.
DR VEuPathDB; HostDB:ENSMUSG00000032607; -.
DR eggNOG; KOG2770; Eukaryota.
DR GeneTree; ENSGT00940000157524; -.
DR HOGENOM; CLU_007884_10_0_1; -.
DR InParanoid; Q8CFA2; -.
DR OMA; MPVQYPA; -.
DR OrthoDB; 673132at2759; -.
DR PhylomeDB; Q8CFA2; -.
DR TreeFam; TF313026; -.
DR Reactome; R-MMU-6783984; Glycine degradation.
DR BioGRID-ORCS; 434437; 1 hit in 72 CRISPR screens.
DR ChiTaRS; Amt; mouse.
DR PRO; PR:Q8CFA2; -.
DR Proteomes; UP000000589; Chromosome 9.
DR RNAct; Q8CFA2; protein.
DR Bgee; ENSMUSG00000032607; Expressed in yolk sac and 87 other tissues.
DR ExpressionAtlas; Q8CFA2; baseline and differential.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0004047; F:aminomethyltransferase activity; ISS:UniProtKB.
DR GO; GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
DR GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; ISS:UniProtKB.
DR Gene3D; 3.30.1360.120; -; 1.
DR InterPro; IPR006223; GCS_T.
DR InterPro; IPR028896; GCST/YgfZ/DmdA.
DR InterPro; IPR013977; GCV_T_C.
DR InterPro; IPR006222; GCV_T_N.
DR InterPro; IPR029043; GcvT/YgfZ_C.
DR InterPro; IPR027266; TrmE/GcvT_dom1.
DR PANTHER; PTHR43757; PTHR43757; 1.
DR Pfam; PF01571; GCV_T; 1.
DR Pfam; PF08669; GCV_T_C; 1.
DR SUPFAM; SSF101790; SSF101790; 1.
DR TIGRFAMs; TIGR00528; gcvT; 1.
PE 1: Evidence at protein level;
KW Aminotransferase; Mitochondrion; Reference proteome; Transferase;
KW Transit peptide.
FT TRANSIT 1..28
FT /note="Mitochondrion"
FT /evidence="ECO:0000250|UniProtKB:P25285"
FT CHAIN 29..403
FT /note="Aminomethyltransferase, mitochondrial"
FT /id="PRO_0000010756"
FT BINDING 232
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P48728"
FT BINDING 261
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P48728"
FT BINDING 399
FT /ligand="substrate"
FT /evidence="ECO:0000250|UniProtKB:P48728"
FT MOD_RES 368
FT /note="N6-succinyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
SQ SEQUENCE 403 AA; 44009 MW; 4FDEC0DC81492167 CRC64;
MHRIVSVVAP LGFRLQAQPL VQSRPLSSVQ DVLRRTPLYD FHLAHGGKMV AFAGWSLPVQ
YRDSHVDSHL HTRRHCSLFD VSHMLQTKIF GCDRVKLLES VVVGDIAELR PNQGTLSLFT
NEAGGILDDL IVSNTSEGHL YVVSNAGCRD KDLALMQDKV KEFQNRGLDV GLEVVENALL
ALQGPTATQV LQAGVTDDMK KLPFMTSAVM EVFGVSGCRV TRCGYTGEDG VEISVPATGA
VHLATTLLKN PEVKLAGLAA RDSLRLEAGL CLYGNDIDEH TTPVEGSLSW TLGKRRRIAM
DFPGAKIIVP QLKGEVQRRR VGLICEGAPV RAHSPILNTE GTVIGTVTSG CPSPSLKKNV
AMGYVPFKYS RPGTQLLVEV RRKQQMTVVS KMPFVPTNYY TLK
