ID   GCST_NOCFA              Reviewed;         366 AA.
AC   Q5YZ49;
DT   15-FEB-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-NOV-2004, sequence version 1.
DT   03-AUG-2022, entry version 101.
DE   RecName: Full=Aminomethyltransferase {ECO:0000255|HAMAP-Rule:MF_00259};
DE            EC=2.1.2.10 {ECO:0000255|HAMAP-Rule:MF_00259};
DE   AltName: Full=Glycine cleavage system T protein {ECO:0000255|HAMAP-Rule:MF_00259};
GN   Name=gcvT {ECO:0000255|HAMAP-Rule:MF_00259}; OrderedLocusNames=NFA_16960;
OS   Nocardia farcinica (strain IFM 10152).
OC   Bacteria; Actinobacteria; Corynebacteriales; Nocardiaceae; Nocardia.
OX   NCBI_TaxID=247156;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IFM 10152;
RX   PubMed=15466710; DOI=10.1073/pnas.0406410101;
RA   Ishikawa J., Yamashita A., Mikami Y., Hoshino Y., Kurita H., Hotta K.,
RA   Shiba T., Hattori M.;
RT   "The complete genomic sequence of Nocardia farcinica IFM 10152.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:14925-14930(2004).
CC   -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC       glycine. {ECO:0000255|HAMAP-Rule:MF_00259}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-
CC         aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-
CC         5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +
CC         NH4(+); Xref=Rhea:RHEA:16945, Rhea:RHEA-COMP:10475, Rhea:RHEA-
CC         COMP:10492, ChEBI:CHEBI:15636, ChEBI:CHEBI:28938, ChEBI:CHEBI:57453,
CC         ChEBI:CHEBI:83100, ChEBI:CHEBI:83143; EC=2.1.2.10;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00259};
CC   -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC       T, L and H. {ECO:0000255|HAMAP-Rule:MF_00259}.
CC   -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000255|HAMAP-
CC       Rule:MF_00259}.
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DR   EMBL; AP006618; BAD56542.1; -; Genomic_DNA.
DR   RefSeq; WP_011208227.1; NC_006361.1.
DR   AlphaFoldDB; Q5YZ49; -.
DR   SMR; Q5YZ49; -.
DR   STRING; 247156.NFA_16960; -.
DR   EnsemblBacteria; BAD56542; BAD56542; NFA_16960.
DR   GeneID; 61132480; -.
DR   KEGG; nfa:NFA_16960; -.
DR   eggNOG; COG0404; Bacteria.
DR   HOGENOM; CLU_007884_10_2_11; -.
DR   OMA; YPLHGQD; -.
DR   BioCyc; NFAR247156:NFA_RS08550-MON; -.
DR   Proteomes; UP000006820; Chromosome.
DR   GO; GO:0004047; F:aminomethyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.1360.120; -; 1.
DR   HAMAP; MF_00259; GcvT; 1.
DR   InterPro; IPR006223; GCS_T.
DR   InterPro; IPR022903; GCS_T_bac.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   PANTHER; PTHR43757; PTHR43757; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   SUPFAM; SSF101790; SSF101790; 1.
DR   TIGRFAMs; TIGR00528; gcvT; 1.
PE   3: Inferred from homology;
KW   Aminotransferase; Reference proteome; Transferase.
FT   CHAIN           1..366
FT                   /note="Aminomethyltransferase"
FT                   /id="PRO_0000122578"
SQ   SEQUENCE   366 AA;  38896 MW;  2B0AA8C08B216FEF CRC64;
     MTETDLLRGP VHDLHGELGA TFAEFGGWQM PVAYAGTVGE HQAVRTTVGL FDVSHLGKAT
     VAGPGAAQFV NATLTNDLGR IHPGKAQYTL CCSADGGVID DLIAYYVADD EIFLVPNAAN
     TAAVVAELAA AAPAGVTVTD QHRDYAVFAV QGPRSAQVLA ALGLPTEMEY MAFADAAWDG
     RPIRVCRTGY TGEHGYEVLP RWADAEPVFR ALLEQVRAAD GQPAGLGARD TLRTEMGYPL
     HGHELSREIS PVQARTGWAV GWRKPQFWGK AALEQEKAAG PRRILMGLKA IDRGVLRQGQ
     PVLRDGEPVG ETTSGTFSPT LKVGIALALL DTGAGLEPGA EVAVDVRGRR LRCEVVKPPF
     VPSRTS