ID   GCST_PSEAE              Reviewed;         360 AA.
AC   Q9HTX5;
DT   01-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Aminomethyltransferase {ECO:0000255|HAMAP-Rule:MF_00259};
DE            EC=2.1.2.10 {ECO:0000255|HAMAP-Rule:MF_00259};
DE   AltName: Full=Glycine cleavage system T protein {ECO:0000255|HAMAP-Rule:MF_00259};
GN   Name=gcvT {ECO:0000255|HAMAP-Rule:MF_00259}; OrderedLocusNames=PA5215;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
CC   -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC       glycine. {ECO:0000255|HAMAP-Rule:MF_00259}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-
CC         aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-
CC         5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +
CC         NH4(+); Xref=Rhea:RHEA:16945, Rhea:RHEA-COMP:10475, Rhea:RHEA-
CC         COMP:10492, ChEBI:CHEBI:15636, ChEBI:CHEBI:28938, ChEBI:CHEBI:57453,
CC         ChEBI:CHEBI:83100, ChEBI:CHEBI:83143; EC=2.1.2.10;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00259};
CC   -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC       T, L and H. {ECO:0000255|HAMAP-Rule:MF_00259}.
CC   -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000255|HAMAP-
CC       Rule:MF_00259}.
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DR   EMBL; AE004091; AAG08600.1; -; Genomic_DNA.
DR   PIR; G82994; G82994.
DR   RefSeq; NP_253902.1; NC_002516.2.
DR   RefSeq; WP_003114049.1; NZ_QZGE01000002.1.
DR   AlphaFoldDB; Q9HTX5; -.
DR   SMR; Q9HTX5; -.
DR   STRING; 287.DR97_2584; -.
DR   PaxDb; Q9HTX5; -.
DR   PRIDE; Q9HTX5; -.
DR   DNASU; 880638; -.
DR   EnsemblBacteria; AAG08600; AAG08600; PA5215.
DR   GeneID; 880638; -.
DR   KEGG; pae:PA5215; -.
DR   PATRIC; fig|208964.12.peg.5465; -.
DR   PseudoCAP; PA5215; -.
DR   HOGENOM; CLU_007884_10_2_6; -.
DR   InParanoid; Q9HTX5; -.
DR   OMA; YPLHGQD; -.
DR   PhylomeDB; Q9HTX5; -.
DR   BioCyc; PAER208964:G1FZ6-5334-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0004047; F:aminomethyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.1360.120; -; 1.
DR   HAMAP; MF_00259; GcvT; 1.
DR   InterPro; IPR006223; GCS_T.
DR   InterPro; IPR022903; GCS_T_bac.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   PANTHER; PTHR43757; PTHR43757; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   SUPFAM; SSF101790; SSF101790; 1.
DR   TIGRFAMs; TIGR00528; gcvT; 1.
PE   3: Inferred from homology;
KW   Aminotransferase; Reference proteome; Transferase.
FT   CHAIN           1..360
FT                   /note="Aminomethyltransferase"
FT                   /id="PRO_0000122586"
SQ   SEQUENCE   360 AA;  38907 MW;  F713824A06002530 CRC64;
     MGLRTPLYEL HVALGAKMVD FGGWDMPLHY GSQVEEHHQV RRDCGVFDVS HMTVVDVAGE
     QATAYLQHLL ANDVARLGET GKALYSAMLN EEGGVVDDLI VYLTEHGYRV VVNASTRDKD
     IAWMQAQAAG FKVDLQERGD LAMLAIQGPN ARVHSSELVS PARAALIREL KPFQGRAEGD
     WFIARTGYTG EDGLEIMLPA AEAPGFLNEL VGAGISPAGL GARDTLRLEA GLNLYGQDMD
     ESVSPLAANM GWTVAWEPVA RDFVGRRALE AQKAAGDQPK LVGLVLEERG VLRAHQVVRV
     AGIGEGEITS GSFSPTLNKS IALARVPAAT GDRAEVEIRG KWYPVRVVQP SFVRHGKPLI