ID   GCST_PYRAB              Reviewed;         398 AA.
AC   Q9UZP8; G8ZJQ1;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 123.
DE   RecName: Full=Probable aminomethyltransferase {ECO:0000255|HAMAP-Rule:MF_00259};
DE            EC=2.1.2.10 {ECO:0000255|HAMAP-Rule:MF_00259};
DE   AltName: Full=Glycine cleavage system T protein {ECO:0000255|HAMAP-Rule:MF_00259};
GN   Name=gcvT {ECO:0000255|HAMAP-Rule:MF_00259}; OrderedLocusNames=PYRAB10970;
GN   ORFNames=PAB1638;
OS   Pyrococcus abyssi (strain GE5 / Orsay).
OC   Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC   Pyrococcus.
OX   NCBI_TaxID=272844;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=GE5 / Orsay;
RX   PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA   Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA   Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA   Weissenbach J., Zivanovic Y., Forterre P.;
RT   "An integrated analysis of the genome of the hyperthermophilic archaeon
RT   Pyrococcus abyssi.";
RL   Mol. Microbiol. 47:1495-1512(2003).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=GE5 / Orsay;
RX   PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA   Gao J., Wang J.;
RT   "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT   Pyrococcus furiosus DSM 3638.";
RL   Curr. Microbiol. 64:118-129(2012).
CC   -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC       glycine. {ECO:0000255|HAMAP-Rule:MF_00259}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-
CC         aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-
CC         5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +
CC         NH4(+); Xref=Rhea:RHEA:16945, Rhea:RHEA-COMP:10475, Rhea:RHEA-
CC         COMP:10492, ChEBI:CHEBI:15636, ChEBI:CHEBI:28938, ChEBI:CHEBI:57453,
CC         ChEBI:CHEBI:83100, ChEBI:CHEBI:83143; EC=2.1.2.10;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00259};
CC   -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC       T, L and H. {ECO:0000255|HAMAP-Rule:MF_00259}.
CC   -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000255|HAMAP-
CC       Rule:MF_00259}.
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DR   EMBL; AJ248286; CAB50008.1; -; Genomic_DNA.
DR   EMBL; HE613800; CCE70510.1; -; Genomic_DNA.
DR   PIR; C75088; C75088.
DR   RefSeq; WP_010868215.1; NC_000868.1.
DR   AlphaFoldDB; Q9UZP8; -.
DR   SMR; Q9UZP8; -.
DR   STRING; 272844.PAB1638; -.
DR   EnsemblBacteria; CAB50008; CAB50008; PAB1638.
DR   GeneID; 1496455; -.
DR   KEGG; pab:PAB1638; -.
DR   PATRIC; fig|272844.11.peg.1153; -.
DR   eggNOG; arCOG00756; Archaea.
DR   HOGENOM; CLU_007884_10_2_2; -.
DR   OMA; MPVQYPA; -.
DR   OrthoDB; 25548at2157; -.
DR   PhylomeDB; Q9UZP8; -.
DR   Proteomes; UP000000810; Chromosome.
DR   Proteomes; UP000009139; Chromosome.
DR   GO; GO:0004047; F:aminomethyltransferase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.1360.120; -; 1.
DR   HAMAP; MF_00259; GcvT; 1.
DR   InterPro; IPR006223; GCS_T.
DR   InterPro; IPR022903; GCS_T_bac.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   PANTHER; PTHR43757; PTHR43757; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   SUPFAM; SSF101790; SSF101790; 1.
DR   TIGRFAMs; TIGR00528; gcvT; 1.
PE   3: Inferred from homology;
KW   Aminotransferase; Transferase.
FT   CHAIN           1..398
FT                   /note="Probable aminomethyltransferase"
FT                   /id="PRO_0000122623"
SQ   SEQUENCE   398 AA;  45739 MW;  ADF7CED644F9A76F CRC64;
     MAKRVHLFDW HKEHAKKIEE FAGWEMPIWY SSIKEEHLAV RNAVGLFDVS HMGEIYFRGK
     DALKFLQYVT TNDISKPPAI SGIYTLVLNE RGAIKDETLI FNMGNNEYLM ICDSDAFEKL
     YAWFTYLKKT IEQFTKLDLE IELKTYDIAM FAVQGPKARD LARDLFGIDI NEMWWFQARW
     VELDGIKMLL SRSGYTGENG FEVYIEDANP YHPDESKRGE PEKALHVWER ILEEGKKYGI
     KPAGLGARDT LRLEAGYTLY GNETKELQLL STDIDEVTPL QANLEFAIYW DKDFIGKDAL
     LKQKERGLGR KLVHFKMVDK GIPREGYKVY ANGELIGEVT SGTLSPLLNV GIGIAFVKEE
     YAKPGIEIEV EIRGARKKAI TVTPPFYDPK KYGLFRET