GCST_PYRHO
ID GCST_PYRHO Reviewed; 398 AA.
AC O58888;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 15-FEB-2005, sequence version 2.
DT 03-AUG-2022, entry version 133.
DE RecName: Full=Probable aminomethyltransferase {ECO:0000255|HAMAP-Rule:MF_00259};
DE EC=2.1.2.10 {ECO:0000255|HAMAP-Rule:MF_00259};
DE AltName: Full=Glycine cleavage system T protein {ECO:0000255|HAMAP-Rule:MF_00259};
GN Name=gcvT {ECO:0000255|HAMAP-Rule:MF_00259}; OrderedLocusNames=PH1146;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC glycine. {ECO:0000255|HAMAP-Rule:MF_00259}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-
CC aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-
CC 5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +
CC NH4(+); Xref=Rhea:RHEA:16945, Rhea:RHEA-COMP:10475, Rhea:RHEA-
CC COMP:10492, ChEBI:CHEBI:15636, ChEBI:CHEBI:28938, ChEBI:CHEBI:57453,
CC ChEBI:CHEBI:83100, ChEBI:CHEBI:83143; EC=2.1.2.10;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00259};
CC -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC T, L and H. {ECO:0000255|HAMAP-Rule:MF_00259}.
CC -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000255|HAMAP-
CC Rule:MF_00259}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA30246.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; BA000001; BAA30246.1; ALT_INIT; Genomic_DNA.
DR PIR; D71056; D71056.
DR RefSeq; WP_048053320.1; NC_000961.1.
DR PDB; 1V5V; X-ray; 1.50 A; A/B=1-398.
DR PDBsum; 1V5V; -.
DR AlphaFoldDB; O58888; -.
DR SMR; O58888; -.
DR STRING; 70601.3257563; -.
DR EnsemblBacteria; BAA30246; BAA30246; BAA30246.
DR GeneID; 1443465; -.
DR KEGG; pho:PH1146; -.
DR eggNOG; arCOG00756; Archaea.
DR OMA; MPVQYPA; -.
DR OrthoDB; 25548at2157; -.
DR BRENDA; 1.4.1.27; 5244.
DR EvolutionaryTrace; O58888; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0004047; F:aminomethyltransferase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
DR GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IEA:UniProtKB-UniRule.
DR Gene3D; 3.30.1360.120; -; 1.
DR HAMAP; MF_00259; GcvT; 1.
DR InterPro; IPR006223; GCS_T.
DR InterPro; IPR022903; GCS_T_bac.
DR InterPro; IPR028896; GCST/YgfZ/DmdA.
DR InterPro; IPR013977; GCV_T_C.
DR InterPro; IPR006222; GCV_T_N.
DR InterPro; IPR029043; GcvT/YgfZ_C.
DR InterPro; IPR027266; TrmE/GcvT_dom1.
DR PANTHER; PTHR43757; PTHR43757; 1.
DR Pfam; PF01571; GCV_T; 1.
DR Pfam; PF08669; GCV_T_C; 1.
DR SUPFAM; SSF101790; SSF101790; 1.
DR TIGRFAMs; TIGR00528; gcvT; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminotransferase; Transferase.
FT CHAIN 1..398
FT /note="Probable aminomethyltransferase"
FT /id="PRO_0000122625"
FT HELIX 8..14
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 16..21
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 24..31
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 33..42
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 45..48
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 52..59
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 62..69
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 70..72
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 79..88
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 94..104
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 107..112
FT /evidence="ECO:0007829|PDB:1V5V"
FT TURN 114..116
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 117..132
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 140..143
FT /evidence="ECO:0007829|PDB:1V5V"
FT TURN 145..147
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 148..155
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 158..166
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 170..172
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 177..183
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 186..191
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 195..198
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 200..206
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 215..217
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 222..235
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 236..238
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 241..243
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 245..255
FT /evidence="ECO:0007829|PDB:1V5V"
FT TURN 260..262
FT /evidence="ECO:0007829|PDB:1V5V"
FT TURN 279..283
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 285..287
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 297..306
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 310..320
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 328..331
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 334..344
FT /evidence="ECO:0007829|PDB:1V5V"
FT TURN 346..348
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 350..358
FT /evidence="ECO:0007829|PDB:1V5V"
FT HELIX 359..361
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 367..372
FT /evidence="ECO:0007829|PDB:1V5V"
FT STRAND 375..382
FT /evidence="ECO:0007829|PDB:1V5V"
FT TURN 389..391
FT /evidence="ECO:0007829|PDB:1V5V"
SQ SEQUENCE 398 AA; 45799 MW; C05FE8A757346DFE CRC64;
MVKRVHIFDW HKEHARKIEE FAGWEMPIWY SSIKEEHLAV RNAVGIFDVS HMGEIVFRGK
DALKFLQYVT TNDISKPPAI SGTYTLVLNE RGAIKDETLV FNMGNNEYLM ICDSDAFEKL
YAWFTYLKRT IEQFTKLDLE IELKTYDIAM FAVQGPKARD LAKDLFGIDI NEMWWFQARW
VELDGIKMLL SRSGYTGENG FEVYIEDANP YHPDESKRGE PEKALHVWER ILEEGKKYGI
KPCGLGARDT LRLEAGYTLY GNETKELQLL STDIDEVTPL QANLEFAIYW DKDFIGKDAL
LKQKERGVGR KLVHFKMIDK GIPREGYKVY ANGEMIGEVT SGTLSPLLNV GIGIAFVKEE
YAKPGIEIEV EIRGQRKKAV TVTPPFYDPK KYGLFRET
