ID   GCST_SOLTU              Reviewed;         406 AA.
AC   P54260;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   03-AUG-2022, entry version 109.
DE   RecName: Full=Aminomethyltransferase, mitochondrial;
DE            EC=2.1.2.10;
DE   AltName: Full=Glycine cleavage system T protein;
DE            Short=GCVT;
DE   Flags: Precursor;
GN   Name=GDCST;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Leaf;
RX   PubMed=8165246; DOI=10.1104/pp.104.3.1079;
RA   Kopriva S., Bauwe H.;
RT   "T-protein of glycine decarboxylase from Solanum tuberosum.";
RL   Plant Physiol. 104:1079-1080(1994).
CC   -!- FUNCTION: The glycine cleavage system catalyzes the degradation of
CC       glycine.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-5,6,7,8-tetrahydrofolate + (R)-N(6)-(S(8)-
CC         aminomethyldihydrolipoyl)-L-lysyl-[protein] = (6R)-5,10-methylene-
CC         5,6,7,8-tetrahydrofolate + (R)-N(6)-dihydrolipoyl-L-lysyl-[protein] +
CC         NH4(+); Xref=Rhea:RHEA:16945, Rhea:RHEA-COMP:10475, Rhea:RHEA-
CC         COMP:10492, ChEBI:CHEBI:15636, ChEBI:CHEBI:28938, ChEBI:CHEBI:57453,
CC         ChEBI:CHEBI:83100, ChEBI:CHEBI:83143; EC=2.1.2.10;
CC   -!- SUBUNIT: The glycine cleavage system is composed of four proteins: P,
CC       T, L and H.
CC   -!- SUBCELLULAR LOCATION: Mitochondrion.
CC   -!- SIMILARITY: Belongs to the GcvT family. {ECO:0000305}.
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DR   EMBL; Z25862; CAA81081.1; -; mRNA.
DR   PIR; S59948; S59948.
DR   RefSeq; NP_001275291.1; NM_001288362.1.
DR   AlphaFoldDB; P54260; -.
DR   SMR; P54260; -.
DR   IntAct; P54260; 1.
DR   STRING; 4113.PGSC0003DMT400042406; -.
DR   PRIDE; P54260; -.
DR   ProMEX; P54260; -.
DR   GeneID; 102602939; -.
DR   KEGG; sot:102602939; -.
DR   eggNOG; KOG2770; Eukaryota.
DR   InParanoid; P54260; -.
DR   OrthoDB; 673132at2759; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; P54260; baseline and differential.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0004047; F:aminomethyltransferase activity; IBA:GO_Central.
DR   GO; GO:0008483; F:transaminase activity; IEA:UniProtKB-KW.
DR   GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IBA:GO_Central.
DR   Gene3D; 3.30.1360.120; -; 1.
DR   InterPro; IPR006223; GCS_T.
DR   InterPro; IPR028896; GCST/YgfZ/DmdA.
DR   InterPro; IPR013977; GCV_T_C.
DR   InterPro; IPR006222; GCV_T_N.
DR   InterPro; IPR029043; GcvT/YgfZ_C.
DR   InterPro; IPR027266; TrmE/GcvT_dom1.
DR   PANTHER; PTHR43757; PTHR43757; 1.
DR   Pfam; PF01571; GCV_T; 1.
DR   Pfam; PF08669; GCV_T_C; 1.
DR   SUPFAM; SSF101790; SSF101790; 1.
DR   TIGRFAMs; TIGR00528; gcvT; 1.
PE   2: Evidence at transcript level;
KW   Aminotransferase; Mitochondrion; Reference proteome; Transferase;
KW   Transit peptide.
FT   TRANSIT         1..29
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000250"
FT   CHAIN           30..406
FT                   /note="Aminomethyltransferase, mitochondrial"
FT                   /id="PRO_0000010764"
FT   BINDING         234
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         265
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         403
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   406 AA;  44277 MW;  F52F4A13D70E33AD CRC64;
     MRGGLWQLGQ SITRRLAQAD KKTIGRRCFA SDADLKKTVL YDFHVVNGGK MVPFAGWSMP
     IQYKDSIMDS TVNCRENGSL FDVSHMCGLS LKGKDTIPFL EKLVIADVAG LAPGTGSLTV
     FTNEKGGAID DSVVTKVTND HIYLVVNAGC RDKDLAHIEE HMKSFKSKGG DVSWHIHDER
     SLLALQGPLA APVLQYLTKD DLSKMYFGEF RVLDINGAPC FLTRTGYTGE DGFEISVPSE
     NALDLAKALL EKSEGKIRLT GLGARDSLRL EAGLCLYGND MEQHTTPVEA GLTWAIGKRR
     RAEGGFLGAE VILKQIEEGP KIRRVGFFSS GPPPRSHSEI QDSNGQNIGE ITSGGFSPCL
     KKNIAMGYVK TGNHKAGTNV KIVIRGKSYD GVVTKMPFVP TKYYKP