ID   GCVA_ECO57              Reviewed;         305 AA.
AC   P0A9F8; P32064;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Glycine cleavage system transcriptional activator;
DE   AltName: Full=Gcv operon activator;
GN   Name=gcvA; OrderedLocusNames=Z4125, ECs3668;
OS   Escherichia coli O157:H7.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83334;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX   PubMed=11206551; DOI=10.1038/35054089;
RA   Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA   Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA   Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA   Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA   Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA   Blattner F.R.;
RT   "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL   Nature 409:529-533(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX   PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA   Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA   Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA   Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA   Shiba T., Hattori M., Shinagawa H.;
RT   "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT   genomic comparison with a laboratory strain K-12.";
RL   DNA Res. 8:11-22(2001).
CC   -!- FUNCTION: Regulatory protein for the glycine cleavage system operon
CC       (gcv). Mediates activation of gcv by glycine and repression by purines.
CC       GcvA is negatively autoregulated. Binds to three sites upstream of the
CC       gcv promoter (By similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; AE005174; AAG57922.1; -; Genomic_DNA.
DR   EMBL; BA000007; BAB37091.1; -; Genomic_DNA.
DR   PIR; D91087; D91087.
DR   PIR; F85932; F85932.
DR   RefSeq; NP_311695.1; NC_002695.1.
DR   RefSeq; WP_000044401.1; NZ_SWKA01000005.1.
DR   AlphaFoldDB; P0A9F8; -.
DR   SMR; P0A9F8; -.
DR   STRING; 155864.EDL933_3989; -.
DR   EnsemblBacteria; AAG57922; AAG57922; Z4125.
DR   EnsemblBacteria; BAB37091; BAB37091; ECs_3668.
DR   GeneID; 67413924; -.
DR   GeneID; 916524; -.
DR   KEGG; ece:Z4125; -.
DR   KEGG; ecs:ECs_3668; -.
DR   PATRIC; fig|386585.9.peg.3834; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_37_1_6; -.
DR   OMA; IAIYYGK; -.
DR   Proteomes; UP000000558; Chromosome.
DR   Proteomes; UP000002519; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IEA:InterPro.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   PRINTS; PR00039; HTHLYSR.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   3: Inferred from homology;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..305
FT                   /note="Glycine cleavage system transcriptional activator"
FT                   /id="PRO_0000105626"
FT   DOMAIN          6..63
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        23..42
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   305 AA;  34402 MW;  42AE40C64DAB5F72 CRC64;
     MSKRLPPLNA LRVFDAAARH LSFTRAAEEL FVTQAAVSHQ IKSLEDFLGL KLFRRRNRSL
     LLTEEGQSYF LDIKEIFSQL TEATRKLQAR SAKGALTVSL LPSFAIHWLV PRLSSFNSAY
     PGIDVRIQAV DRQEDKLADD VDVAIFYGRG NWPGLRVEKL YAEYLLPVCS PLLLTGEKPL
     KTPEDLAKHT LLHDASRRDW QTYTRQLGLN HINVQQGPIF SHSAMVLQAA IHGQGVALAN
     NVMAQSEIEA GRLVCPFNDV LVSKNAFYLV CHDSQAELGK IAAFRQWILA KAAAEQEKFR
     FRYEQ