ID   GCVA_ECOLI              Reviewed;         305 AA.
AC   P0A9F6; P32064; Q2MA26;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2005, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Glycine cleavage system transcriptional activator;
DE   AltName: Full=Gcv operon activator;
GN   Name=gcvA; OrderedLocusNames=b2808, JW2779;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
RC   STRAIN=K12;
RX   PubMed=8188587; DOI=10.1128/jb.176.10.2862-2868.1994;
RA   Wilson R.L., Stauffer G.V.;
RT   "DNA sequence and characterization of GcvA, a LysR family regulatory
RT   protein for the Escherichia coli glycine cleavage enzyme system.";
RL   J. Bacteriol. 176:2862-2868(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=K12;
RX   PubMed=7704273; DOI=10.1099/13500872-141-2-419;
RA   Everett M.J., Walsh T., Guay G., Bennett P.M.;
RT   "GcvA, a LysR-type transcriptional regulator protein, activates expression
RT   of the cloned Citrobacter freundii ampC beta-lactamase gene in Escherichia
RT   coli: cross-talk between DNA-binding proteins.";
RL   Microbiology 141:419-430(1995).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
CC   -!- FUNCTION: Regulatory protein for the glycine cleavage system operon
CC       (gcv). Mediates activation of gcv by glycine and repression by purines.
CC       GcvA is negatively autoregulated. Binds to three sites upstream of the
CC       gcv promoter.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm.
CC   -!- SIMILARITY: Belongs to the LysR transcriptional regulatory family.
CC       {ECO:0000305}.
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DR   EMBL; U01030; AAC13742.1; -; Genomic_DNA.
DR   EMBL; X73413; CAA51813.1; -; Genomic_DNA.
DR   EMBL; U29581; AAB40458.1; -; Genomic_DNA.
DR   EMBL; U00096; AAC75850.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76880.1; -; Genomic_DNA.
DR   PIR; I41065; I41065.
DR   RefSeq; NP_417288.1; NC_000913.3.
DR   RefSeq; WP_000044401.1; NZ_STEB01000030.1.
DR   AlphaFoldDB; P0A9F6; -.
DR   SMR; P0A9F6; -.
DR   BioGRID; 4261124; 25.
DR   BioGRID; 851607; 1.
DR   DIP; DIP-9751N; -.
DR   IntAct; P0A9F6; 2.
DR   STRING; 511145.b2808; -.
DR   jPOST; P0A9F6; -.
DR   PaxDb; P0A9F6; -.
DR   PRIDE; P0A9F6; -.
DR   EnsemblBacteria; AAC75850; AAC75850; b2808.
DR   EnsemblBacteria; BAE76880; BAE76880; BAE76880.
DR   GeneID; 67413924; -.
DR   GeneID; 947278; -.
DR   KEGG; ecj:JW2779; -.
DR   KEGG; eco:b2808; -.
DR   PATRIC; fig|1411691.4.peg.3925; -.
DR   EchoBASE; EB1743; -.
DR   eggNOG; COG0583; Bacteria.
DR   HOGENOM; CLU_039613_37_1_6; -.
DR   InParanoid; P0A9F6; -.
DR   OMA; IAIYYGK; -.
DR   PhylomeDB; P0A9F6; -.
DR   BioCyc; EcoCyc:PD00339; -.
DR   PRO; PR:P0A9F6; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0006355; P:regulation of transcription, DNA-templated; IDA:EcoliWiki.
DR   GO; GO:0009411; P:response to UV; IMP:EcoCyc.
DR   GO; GO:0006351; P:transcription, DNA-templated; IDA:EcoCyc.
DR   Gene3D; 1.10.10.10; -; 1.
DR   InterPro; IPR005119; LysR_subst-bd.
DR   InterPro; IPR000847; Tscrpt_reg_HTH_LysR.
DR   InterPro; IPR036388; WH-like_DNA-bd_sf.
DR   InterPro; IPR036390; WH_DNA-bd_sf.
DR   Pfam; PF00126; HTH_1; 1.
DR   Pfam; PF03466; LysR_substrate; 1.
DR   PRINTS; PR00039; HTHLYSR.
DR   SUPFAM; SSF46785; SSF46785; 1.
DR   PROSITE; PS50931; HTH_LYSR; 1.
PE   1: Evidence at protein level;
KW   Activator; Cytoplasm; DNA-binding; Reference proteome; Repressor;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..305
FT                   /note="Glycine cleavage system transcriptional activator"
FT                   /id="PRO_0000105625"
FT   DOMAIN          6..63
FT                   /note="HTH lysR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
FT   DNA_BIND        23..42
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00253"
SQ   SEQUENCE   305 AA;  34402 MW;  42AE40C64DAB5F72 CRC64;
     MSKRLPPLNA LRVFDAAARH LSFTRAAEEL FVTQAAVSHQ IKSLEDFLGL KLFRRRNRSL
     LLTEEGQSYF LDIKEIFSQL TEATRKLQAR SAKGALTVSL LPSFAIHWLV PRLSSFNSAY
     PGIDVRIQAV DRQEDKLADD VDVAIFYGRG NWPGLRVEKL YAEYLLPVCS PLLLTGEKPL
     KTPEDLAKHT LLHDASRRDW QTYTRQLGLN HINVQQGPIF SHSAMVLQAA IHGQGVALAN
     NVMAQSEIEA GRLVCPFNDV LVSKNAFYLV CHDSQAELGK IAAFRQWILA KAAAEQEKFR
     FRYEQ