ID   GCVHL_STAAM             Reviewed;         110 AA.
AC   A0A0H3JT43;
DT   20-JAN-2016, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   03-AUG-2022, entry version 35.
DE   RecName: Full=Glycine cleavage system H-like protein {ECO:0000303|PubMed:26166706};
DE            Short=GcvH-L {ECO:0000303|PubMed:26166706};
GN   OrderedLocusNames=SAV0324 {ECO:0000312|EMBL:BAB56486.1};
OS   Staphylococcus aureus (strain Mu50 / ATCC 700699).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=158878;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=11418146; DOI=10.1016/s0140-6736(00)04403-2;
RA   Kuroda M., Ohta T., Uchiyama I., Baba T., Yuzawa H., Kobayashi I., Cui L.,
RA   Oguchi A., Aoki K., Nagai Y., Lian J.-Q., Ito T., Kanamori M.,
RA   Matsumaru H., Maruyama A., Murakami H., Hosoyama A., Mizutani-Ui Y.,
RA   Takahashi N.K., Sawano T., Inoue R., Kaito C., Sekimizu K., Hirakawa H.,
RA   Kuhara S., Goto S., Yabuzaki J., Kanehisa M., Yamashita A., Oshima K.,
RA   Furuya K., Yoshino C., Shiba T., Hattori M., Ogasawara N., Hayashi H.,
RA   Hiramatsu K.;
RT   "Whole genome sequencing of meticillin-resistant Staphylococcus aureus.";
RL   Lancet 357:1225-1240(2001).
RN   [2]
RP   LIPOYLATION AT LYS-56, ADP-RIBOSYLATION, MUTAGENESIS OF GLU-53 AND LYS-56,
RP   AND FUNCTION.
RC   STRAIN=Mu50 / ATCC 700699;
RX   PubMed=26166706; DOI=10.1016/j.molcel.2015.06.013;
RA   Rack J.G., Morra R., Barkauskaite E., Kraehenbuehl R., Ariza A., Qu Y.,
RA   Ortmayer M., Leidecker O., Cameron D.R., Matic I., Peleg A.Y., Leys D.,
RA   Traven A., Ahel I.;
RT   "Identification of a class of protein ADP-ribosylating sirtuins in
RT   microbial pathogens.";
RL   Mol. Cell 59:309-320(2015).
CC   -!- FUNCTION: May act as a carrier protein for the ROS scavenging lipoyl
CC       moiety and/or as a substrate for oxidoreductases such as SAV0322 and
CC       SAV0323. {ECO:0000305|PubMed:26166706}.
CC   -!- SUBUNIT: Lipoylated GcvH-L directly interacts with SAV0325, which
CC       reverses the SirTM-mediated mono-ADP-ribosylation of GcvH-L, and with
CC       the oxidoreductase SAV0322. {ECO:0000250|UniProtKB:P0DN69,
CC       ECO:0000305|PubMed:26166706}.
CC   -!- PTM: Is lipoylated on K-56 by LplA2 (SAV0327) and then mono-ADP-
CC       ribosylated, probably on D-27, by SirTM (SAV0326). The mono-ADP-
CC       ribosylation state of GcvH-L might regulate the availability of the
CC       lipoyl moiety for redox reactions; ADP-ribosylation would inhibit the
CC       interaction of the oxidoreductase with GcvH-L when it is not required,
CC       thus ADP-ribosylation of GcvH-L might be acting to keep the response
CC       'off' under non-stress conditions. {ECO:0000269|PubMed:26166706}.
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DR   EMBL; BA000017; BAB56486.1; -; Genomic_DNA.
DR   RefSeq; WP_000731878.1; NC_002758.2.
DR   AlphaFoldDB; A0A0H3JT43; -.
DR   SMR; A0A0H3JT43; -.
DR   PaxDb; A0A0H3JT43; -.
DR   EnsemblBacteria; BAB56486; BAB56486; SAV0324.
DR   KEGG; sav:SAV0324; -.
DR   HOGENOM; CLU_097408_3_0_9; -.
DR   OMA; ENWVMIL; -.
DR   PhylomeDB; A0A0H3JT43; -.
DR   BioCyc; SAUR158878:SAV_RS01805-MON; -.
DR   Proteomes; UP000002481; Chromosome.
DR   GO; GO:0005960; C:glycine cleavage complex; IEA:InterPro.
DR   GO; GO:0019464; P:glycine decarboxylation via glycine cleavage system; IEA:InterPro.
DR   CDD; cd06848; GCS_H; 1.
DR   InterPro; IPR000089; Biotin_lipoyl.
DR   InterPro; IPR002930; GCV_H.
DR   InterPro; IPR033753; GCV_H/Fam206.
DR   InterPro; IPR011053; Single_hybrid_motif.
DR   PANTHER; PTHR11715; PTHR11715; 1.
DR   Pfam; PF01597; GCV_H; 1.
DR   SUPFAM; SSF51230; SSF51230; 1.
DR   PROSITE; PS50968; BIOTINYL_LIPOYL; 1.
PE   1: Evidence at protein level;
KW   ADP-ribosylation; Lipoyl.
FT   CHAIN           1..110
FT                   /note="Glycine cleavage system H-like protein"
FT                   /id="PRO_0000435342"
FT   DOMAIN          10..97
FT                   /note="Lipoyl-binding"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01066"
FT   MOD_RES         27
FT                   /note="ADP-ribosyl aspartic acid"
FT                   /evidence="ECO:0000250|UniProtKB:P0DN69"
FT   MOD_RES         56
FT                   /note="N6-lipoyllysine"
FT                   /evidence="ECO:0000269|PubMed:26166706"
FT   MUTAGEN         53
FT                   /note="E->Q: Decrease in lipoylation."
FT                   /evidence="ECO:0000269|PubMed:26166706"
FT   MUTAGEN         56
FT                   /note="K->R: Loss of lipoylation."
FT                   /evidence="ECO:0000269|PubMed:26166706"
SQ   SEQUENCE   110 AA;  12422 MW;  EB9DCF9FC204CC2A CRC64;
     MKKLANYLWV EKVGDLYVFS MTPELQDDIG TVGYVEFVSP DEVKVDDEIV SIEASKTVID
     VQTPLSGTII ERNTKAEEEP TILNSEKPEE NWLFKLDDVD KEAFLALPEA