GCVR_ECOLI
ID GCVR_ECOLI Reviewed; 190 AA.
AC P0A9I3; O30979; P23483; P77652;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Glycine cleavage system transcriptional repressor;
DE AltName: Full=gcv operon repressor;
GN Name=gcvR; Synonyms=yffD; OrderedLocusNames=b2479, JW2464;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 2-15, AND
RP CHARACTERIZATION.
RC STRAIN=K12;
RX PubMed=9537378; DOI=10.1128/jb.180.7.1803-1807.1998;
RA Ghrist A.C., Stauffer G.V.;
RT "Promoter characterization and constitutive expression of the Escherichia
RT coli gcvR gene.";
RL J. Bacteriol. 180:1803-1807(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9205837; DOI=10.1093/dnares/4.2.91;
RA Yamamoto Y., Aiba H., Baba T., Hayashi K., Inada T., Isono K., Itoh T.,
RA Kimura S., Kitagawa M., Makino K., Miki T., Mitsuhashi N., Mizobuchi K.,
RA Mori H., Nakade S., Nakamura Y., Nashimoto H., Oshima T., Oyama S.,
RA Saito N., Sampei G., Satoh Y., Sivasundaram S., Tagami H., Takahashi H.,
RA Takeda J., Takemoto K., Uehara K., Wada C., Yamagata S., Horiuchi T.;
RT "Construction of a contiguous 874-kb sequence of the Escherichia coli-K12
RT genome corresponding to 50.0-68.8 min on the linkage map and analysis of
RT its sequence features.";
RL DNA Res. 4:91-113(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 11-190.
RC STRAIN=K12;
RX PubMed=2016588; DOI=10.1099/00221287-137-2-361;
RA Andrews S.C., Harrison P.M., Guest J.R.;
RT "A molecular analysis of the 53.3 minute region of the Escherichia coli
RT linkage map.";
RL J. Gen. Microbiol. 137:361-367(1991).
RN [6]
RP SEQUENCE REVISION TO C-TERMINUS.
RX PubMed=9387241; DOI=10.1099/00221287-143-11-3633;
RA Andrews S.C., Berks B.C., McClay J., Ambler A., Quail M.A., Golby P.,
RA Guest J.R.;
RT "A 12-cistron Escherichia coli operon (hyf) encoding a putative proton-
RT translocating formate hydrogenlyase system.";
RL Microbiology 143:3633-3647(1997).
CC -!- FUNCTION: Negative transcriptional regulator of the glycine cleavage
CC system operon (GCV). Does not autoregulate its own expression. It is
CC not yet known how GcvR acts as a repressor. It does not seem to bind
CC DNA. It could interact with GcvA and suppress its activatory activity.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAB88561.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF023337; AAC46232.1; -; Genomic_DNA.
DR EMBL; U00096; AAC75532.2; -; Genomic_DNA.
DR EMBL; AP009048; BAA16356.2; -; Genomic_DNA.
DR EMBL; M63654; AAB88561.1; ALT_INIT; Genomic_DNA.
DR RefSeq; NP_416974.4; NC_000913.3.
DR RefSeq; WP_000176187.1; NZ_STEB01000011.1.
DR AlphaFoldDB; P0A9I3; -.
DR SMR; P0A9I3; -.
DR BioGRID; 4260580; 8.
DR BioGRID; 851289; 2.
DR DIP; DIP-9754N; -.
DR IntAct; P0A9I3; 3.
DR STRING; 511145.b2479; -.
DR jPOST; P0A9I3; -.
DR PaxDb; P0A9I3; -.
DR PRIDE; P0A9I3; -.
DR EnsemblBacteria; AAC75532; AAC75532; b2479.
DR EnsemblBacteria; BAA16356; BAA16356; BAA16356.
DR GeneID; 66673656; -.
DR GeneID; 946950; -.
DR KEGG; ecj:JW2464; -.
DR KEGG; eco:b2479; -.
DR PATRIC; fig|1411691.4.peg.4260; -.
DR EchoBASE; EB1138; -.
DR eggNOG; COG2716; Bacteria.
DR HOGENOM; CLU_095322_0_0_6; -.
DR InParanoid; P0A9I3; -.
DR OMA; TGGTMLN; -.
DR PhylomeDB; P0A9I3; -.
DR BioCyc; EcoCyc:EG11149-MON; -.
DR PRO; PR:P0A9I3; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IEA:InterPro.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:EcoCyc.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR InterPro; IPR002912; ACT_dom.
DR InterPro; IPR016867; GcvR.
DR Pfam; PF01842; ACT; 1.
DR PIRSF; PIRSF028103; GcvR; 1.
DR SUPFAM; SSF55021; SSF55021; 2.
DR PROSITE; PS51671; ACT; 2.
PE 1: Evidence at protein level;
KW Cytoplasm; Direct protein sequencing; Reference proteome; Repeat;
KW Repressor; Transcription; Transcription regulation.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000269|PubMed:9537378"
FT CHAIN 2..190
FT /note="Glycine cleavage system transcriptional repressor"
FT /id="PRO_0000087448"
FT DOMAIN 10..91
FT /note="ACT 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
FT DOMAIN 97..176
FT /note="ACT 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
SQ SEQUENCE 190 AA; 20769 MW; 66A1998E767B556B CRC64;
MTLSSQHYLV ITALGADRPG IVNTITRHVS SCGCNIEDSR LAMLGEEFTF IMLLSGSWNA
ITLIESTLPL KGAELDLLIV MKRTTARPRP PMPASVWVQV DVADSPHLIE RFTALFDAHH
MNIAELVSRT QPAENERAAQ LHIQITAHSP ASADAANIEQ AFKALCTELN AQGSINVVNY
SQHDEQDGVK
