GCY3E_DROME
ID GCY3E_DROME Reviewed; 1163 AA.
AC Q07553; Q9VKI9;
DT 15-NOV-2002, integrated into UniProtKB/Swiss-Prot.
DT 25-JUL-2003, sequence version 4.
DT 03-AUG-2022, entry version 181.
DE RecName: Full=Guanylate cyclase 32E;
DE EC=4.6.1.2;
DE Flags: Precursor;
GN Name=Gyc32E; Synonyms=GC; ORFNames=CG33114;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 59-1163, AND CHARACTERIZATION.
RC STRAIN=Oregon-R; TISSUE=Embryo, and Head;
RX PubMed=8104831; DOI=10.1006/dbio.1993.1255;
RA Gigliotti S., Cavaliere V., Manzi A., Tino A., Graziani F., Malva C.;
RT "A membrane guanylate cyclase Drosophila homolog gene exhibits maternal and
RT zygotic expression.";
RL Dev. Biol. 159:450-461(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=GTP = 3',5'-cyclic GMP + diphosphate; Xref=Rhea:RHEA:13665,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:37565, ChEBI:CHEBI:57746; EC=4.6.1.2;
CC Evidence={ECO:0000303|PubMed:8104831};
CC -!- SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:8104831}.
CC -!- SIMILARITY: Belongs to the adenylyl cyclase class-4/guanylyl cyclase
CC family. {ECO:0000255|PROSITE-ProRule:PRU00099}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAA51319.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AE014134; AAF53079.3; -; Genomic_DNA.
DR EMBL; X72800; CAA51318.1; ALT_FRAME; Genomic_DNA.
DR EMBL; X72801; CAA51319.1; ALT_FRAME; mRNA.
DR PIR; S33525; S33525.
DR RefSeq; NP_788027.1; NM_176013.2.
DR AlphaFoldDB; Q07553; -.
DR SMR; Q07553; -.
DR BioGRID; 60615; 1.
DR IntAct; Q07553; 2.
DR STRING; 7227.FBpp0111769; -.
DR GlyGen; Q07553; 4 sites.
DR PaxDb; Q07553; -.
DR PRIDE; Q07553; -.
DR EnsemblMetazoa; FBtr0343625; FBpp0310220; FBgn0010197.
DR GeneID; 34553; -.
DR KEGG; dme:Dmel_CG33114; -.
DR CTD; 34553; -.
DR FlyBase; FBgn0010197; Gyc32E.
DR VEuPathDB; VectorBase:FBgn0010197; -.
DR eggNOG; KOG1023; Eukaryota.
DR GeneTree; ENSGT00940000169004; -.
DR InParanoid; Q07553; -.
DR PhylomeDB; Q07553; -.
DR SignaLink; Q07553; -.
DR BioGRID-ORCS; 34553; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 34553; -.
DR PRO; PR:Q07553; -.
DR Proteomes; UP000000803; Chromosome 2L.
DR Bgee; FBgn0010197; Expressed in eye disc (Drosophila) and 4 other tissues.
DR ExpressionAtlas; Q07553; baseline and differential.
DR Genevisible; Q07553; DM.
DR GO; GO:0005887; C:integral component of plasma membrane; ISS:FlyBase.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:InterPro.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0004383; F:guanylate cyclase activity; ISS:FlyBase.
DR GO; GO:0001653; F:peptide receptor activity; IBA:GO_Central.
DR GO; GO:0038023; F:signaling receptor activity; ISS:FlyBase.
DR GO; GO:0006182; P:cGMP biosynthetic process; IBA:GO_Central.
DR GO; GO:0019934; P:cGMP-mediated signaling; ISS:FlyBase.
DR GO; GO:0006468; P:protein phosphorylation; IEA:InterPro.
DR GO; GO:0007168; P:receptor guanylyl cyclase signaling pathway; IBA:GO_Central.
DR GO; GO:0007165; P:signal transduction; IBA:GO_Central.
DR CDD; cd07302; CHD; 1.
DR Gene3D; 3.30.70.1230; -; 1.
DR InterPro; IPR001054; A/G_cyclase.
DR InterPro; IPR018297; A/G_cyclase_CS.
DR InterPro; IPR001828; ANF_lig-bd_rcpt.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR029787; Nucleotide_cyclase.
DR InterPro; IPR028082; Peripla_BP_I.
DR InterPro; IPR000719; Prot_kinase_dom.
DR InterPro; IPR001245; Ser-Thr/Tyr_kinase_cat_dom.
DR Pfam; PF01094; ANF_receptor; 1.
DR Pfam; PF00211; Guanylate_cyc; 1.
DR Pfam; PF07714; PK_Tyr_Ser-Thr; 1.
DR SMART; SM00044; CYCc; 1.
DR SUPFAM; SSF53822; SSF53822; 1.
DR SUPFAM; SSF55073; SSF55073; 1.
DR SUPFAM; SSF56112; SSF56112; 1.
DR PROSITE; PS00452; GUANYLATE_CYCLASE_1; 1.
DR PROSITE; PS50125; GUANYLATE_CYCLASE_2; 1.
DR PROSITE; PS50011; PROTEIN_KINASE_DOM; 1.
PE 1: Evidence at protein level;
KW cGMP biosynthesis; Disulfide bond; Glycoprotein; GTP-binding; Lyase;
KW Membrane; Nucleotide-binding; Receptor; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..25
FT /evidence="ECO:0000255"
FT CHAIN 26..1163
FT /note="Guanylate cyclase 32E"
FT /id="PRO_0000012390"
FT TOPO_DOM 29..469
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 470..490
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 491..1163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 507..800
FT /note="Protein kinase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00159"
FT DOMAIN 873..1003
FT /note="Guanylate cyclase"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00099"
FT CARBOHYD 147
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 206
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 368
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 390
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 454
FT /note="Interchain"
FT /evidence="ECO:0000305"
FT CONFLICT 59
FT /note="L -> D (in Ref. 3; CAA51318/CAA51319)"
FT /evidence="ECO:0000305"
FT CONFLICT 678
FT /note="A -> L (in Ref. 3; CAA51318/CAA51319)"
FT /evidence="ECO:0000305"
FT CONFLICT 831..841
FT /note="RTNLLYEEKKK -> ANQFVVRREEE (in Ref. 3; CAA51318)"
FT /evidence="ECO:0000305"
FT CONFLICT 893
FT /note="E -> M (in Ref. 3; CAA51318/CAA51319)"
FT /evidence="ECO:0000305"
FT CONFLICT 910
FT /note="S -> W (in Ref. 3; CAA51318/CAA51319)"
FT /evidence="ECO:0000305"
FT CONFLICT 962
FT /note="P -> R (in Ref. 3; CAA51318/CAA51319)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1163 AA; 130915 MW; 9C0E949DA9FB7E7E CRC64;
MPGPCASAAA FSCILVLLLL GCQRSNPLAA GATVSSMRRL TDTINIGFLA EYSQMRVTLG
GLPLAIEDVN KNPNLLPGKK LAFKPVDIGH KMSAYRVKPL RAMTQMREAG VTAFIGPDES
CTTEALLASA WNTPMLSFKC SDPIVSNKST FHTFARTLAP ASKVSKSVIS LLNAFHWNKF
SIVVSSKPIW GSDVARAIQE LAEARNFTIS HFKYISDYIP TTKTLSQIDK IIEETYATTR
IYVFIGEHIA MVDFVRGLQN RRLLESGDYI VVSVDDEIYD SNRRVNIMER NYLDPYIRKE
KSKSLDKISF RSVIKISMTY PQNPHIRVPI YGLHLYDSVM IYVRAITEVL RLGGDIYDGN
LVMSHIFNRS YHSIQGFDVY IDSNGDAEGN YTVITLQNDV GSGASIGSLA KMSMQPVGFF
AYDKNSVIPE FRYIKNDRPI QWLNGRPPLA EPLCGFHGEL CPRKKLDWRY LVSGPLCALV
VVVAIALLIK HYRYEQTLAG LLWKVDMKDV TVINLGEYNN PTNKNIFQIC RQSILVVGEP
NKRSFTNIAL FRGNIVAMKK IHKKSVDITR SIRKELKLMR EVRHENIINF IGASTDHGSV
IIFTTYCARG SLEDVLANED LHLDHMFISS LVSDILKGMI YLHDSEIISH GNLRSSNCLI
DSRWVCQISD FGLHELKAGQ EEPNKSELEL KRALCMAPEL LRDAYRPGRG SQKGDVYSFG
ILLYEMIGRK GPWGDTAYSK EEIIQFVKCP EMLQHGVFRP ALTHTHLDIP DYIRKCLCQC
WDEDPEVRPD IRLVRMHLKE LQAGLKPNIF DNMLSIMEKY AYNLEGLVQE RTNLLYEEKK
KTDMLLYQML PRPVAELLKR GDPVEAECFD CVTILFSDIV GFTELCTTST PFEVVEMLND
WYTCCDSIIS NYDVYKVETI GDAYMVVSGL PLQNGSRHAG EIASLALHLL ETVGNLKIRH
KPTETVQLRI GVHSGPCAAG VVGQKMPRYC LFGDTVNTAS RMESTGDSMR IHISEATYQL
LQVIGSYVCI ERGLTSIKGK GDMRTYWLTK RQQPELTPDL ISTVDTLDTY CSGPRESMEV
SVHQYCSPAS NNYRLGSCNC DTKCLYSRRS DDNVTNSHGT SEFPKVSEPA QVNCNQLCVC
RLNSSQMFNN RGPRSAPSIT FRL
